Comparison of light microscopy and spatially resolved fourier transform infrared (FT-IR) microscopy in the examination of cell wall components of strawberries

Jaana Suutarinen, Liisa Änäkäinen, Karin Autio

Research output: Contribution to journalArticleScientificpeer-review

39 Citations (Scopus)

Abstract

Different chemical components and their location in strawberry achene, vascular bundles and cortical cell walls were studied by means of bright-field microscopy using different staining systems and by Fourier transform infrared (FT-IR) microscopy and the two methods were compared.
For bright-field microscopy, pectin, protein, lignin and cellulose were stained with specific staining systems. For FT-IR microscopy, the spectra of commercial pectin, protein, lignin and cellulose were measured in the 4000–700 cm−1region and compared to spectra obtained from the different strawberry tissues.
Both methods showed that lignin was an important component of achene and vascular bundles, whereas the cortical cell walls contained mainly pectin and cellulose in the middle lamella, and protein as deposits in the outer layer.
The structure of all the cell walls was complicated and consisted of several components. The two methods used gave comparable results.
Original languageEnglish
Pages (from-to)595-601
JournalLebensmittel-Wissenschaft & Technologie
Volume31
Issue number7-8
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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