Comparison of light microscopy and spatially resolved fourier transform infrared (FT-IR) microscopy in the examination of cell wall components of strawberries

Jaana Suutarinen, Liisa Änäkäinen, Karin Autio

Research output: Contribution to journalArticleScientificpeer-review

37 Citations (Scopus)

Abstract

Different chemical components and their location in strawberry achene, vascular bundles and cortical cell walls were studied by means of bright-field microscopy using different staining systems and by Fourier transform infrared (FT-IR) microscopy and the two methods were compared.
For bright-field microscopy, pectin, protein, lignin and cellulose were stained with specific staining systems. For FT-IR microscopy, the spectra of commercial pectin, protein, lignin and cellulose were measured in the 4000–700 cm−1region and compared to spectra obtained from the different strawberry tissues.
Both methods showed that lignin was an important component of achene and vascular bundles, whereas the cortical cell walls contained mainly pectin and cellulose in the middle lamella, and protein as deposits in the outer layer.
The structure of all the cell walls was complicated and consisted of several components. The two methods used gave comparable results.
Original languageEnglish
Pages (from-to)595-601
JournalLebensmittel-Wissenschaft & Technologie
Volume31
Issue number7-8
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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Fragaria
cell wall components
Fourier Analysis
Cellular Structures
Cell Wall
strawberries
light microscopy
Lignin
Microscopy
microscopy
Cellulose
pectins
Light
lignin
cellulose
cell walls
vascular bundles
Blood Vessels
Staining and Labeling
Proteins

Cite this

@article{c665f5259e7d432788e3d137aa44eeb3,
title = "Comparison of light microscopy and spatially resolved fourier transform infrared (FT-IR) microscopy in the examination of cell wall components of strawberries",
abstract = "Different chemical components and their location in strawberry achene, vascular bundles and cortical cell walls were studied by means of bright-field microscopy using different staining systems and by Fourier transform infrared (FT-IR) microscopy and the two methods were compared. For bright-field microscopy, pectin, protein, lignin and cellulose were stained with specific staining systems. For FT-IR microscopy, the spectra of commercial pectin, protein, lignin and cellulose were measured in the 4000–700 cm−1region and compared to spectra obtained from the different strawberry tissues. Both methods showed that lignin was an important component of achene and vascular bundles, whereas the cortical cell walls contained mainly pectin and cellulose in the middle lamella, and protein as deposits in the outer layer. The structure of all the cell walls was complicated and consisted of several components. The two methods used gave comparable results.",
author = "Jaana Suutarinen and Liisa {\"A}n{\"a}k{\"a}inen and Karin Autio",
year = "1998",
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language = "English",
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Comparison of light microscopy and spatially resolved fourier transform infrared (FT-IR) microscopy in the examination of cell wall components of strawberries. / Suutarinen, Jaana; Änäkäinen, Liisa; Autio, Karin.

In: Lebensmittel-Wissenschaft & Technologie, Vol. 31, No. 7-8, 1998, p. 595-601.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Comparison of light microscopy and spatially resolved fourier transform infrared (FT-IR) microscopy in the examination of cell wall components of strawberries

AU - Suutarinen, Jaana

AU - Änäkäinen, Liisa

AU - Autio, Karin

PY - 1998

Y1 - 1998

N2 - Different chemical components and their location in strawberry achene, vascular bundles and cortical cell walls were studied by means of bright-field microscopy using different staining systems and by Fourier transform infrared (FT-IR) microscopy and the two methods were compared. For bright-field microscopy, pectin, protein, lignin and cellulose were stained with specific staining systems. For FT-IR microscopy, the spectra of commercial pectin, protein, lignin and cellulose were measured in the 4000–700 cm−1region and compared to spectra obtained from the different strawberry tissues. Both methods showed that lignin was an important component of achene and vascular bundles, whereas the cortical cell walls contained mainly pectin and cellulose in the middle lamella, and protein as deposits in the outer layer. The structure of all the cell walls was complicated and consisted of several components. The two methods used gave comparable results.

AB - Different chemical components and their location in strawberry achene, vascular bundles and cortical cell walls were studied by means of bright-field microscopy using different staining systems and by Fourier transform infrared (FT-IR) microscopy and the two methods were compared. For bright-field microscopy, pectin, protein, lignin and cellulose were stained with specific staining systems. For FT-IR microscopy, the spectra of commercial pectin, protein, lignin and cellulose were measured in the 4000–700 cm−1region and compared to spectra obtained from the different strawberry tissues. Both methods showed that lignin was an important component of achene and vascular bundles, whereas the cortical cell walls contained mainly pectin and cellulose in the middle lamella, and protein as deposits in the outer layer. The structure of all the cell walls was complicated and consisted of several components. The two methods used gave comparable results.

U2 - 10.1006/fstl.1997.0331

DO - 10.1006/fstl.1997.0331

M3 - Article

VL - 31

SP - 595

EP - 601

JO - LWT - Food Science and Technology

JF - LWT - Food Science and Technology

SN - 0023-6438

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