Abstract
Two commercial fungal lipase preparations from Aspergillus niger and Penicillium cyclopium, two fungal phospholipases from A. niger (A1 and A2) and one phospholipase from porcine pancreas (A2, Lecitase) were compared in the hydrolysis of soybean phospholipids. The isoelectric points, pH and temperature optima and temperature stabilities of the phospholipases in these enzyme preparations were also determined.
Lipase preparations which also contained phospholipase and lysophospholipase activities were more efficient than phospholipase preparations in the hydrolysis of soybean phospholipids. Phospholipase A1 and lipase preparations released fatty acids from both positions of the substrate molecule and as a result glyceryl-phosphoryl-choline was formed.
Both the A2 phospholipase preparations tested produced lysophospholipids through partial selective hydrolysis of phospholipids.
Lipase preparations which also contained phospholipase and lysophospholipase activities were more efficient than phospholipase preparations in the hydrolysis of soybean phospholipids. Phospholipase A1 and lipase preparations released fatty acids from both positions of the substrate molecule and as a result glyceryl-phosphoryl-choline was formed.
Both the A2 phospholipase preparations tested produced lysophospholipids through partial selective hydrolysis of phospholipids.
| Original language | English |
|---|---|
| Pages (from-to) | 393-401 |
| Journal | Process Biochemistry |
| Volume | 30 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1995 |
| MoE publication type | A1 Journal article-refereed |