Comparison of lipases and phospholipases in the hydrolysis of phospholipids

Annikka Mustranta (Corresponding Author), Pirkko Forssell, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

40 Citations (Scopus)

Abstract

Two commercial fungal lipase preparations from Aspergillus niger and Penicillium cyclopium, two fungal phospholipases from A. niger (A1 and A2) and one phospholipase from porcine pancreas (A2, Lecitase) were compared in the hydrolysis of soybean phospholipids. The isoelectric points, pH and temperature optima and temperature stabilities of the phospholipases in these enzyme preparations were also determined.
Lipase preparations which also contained phospholipase and lysophospholipase activities were more efficient than phospholipase preparations in the hydrolysis of soybean phospholipids. Phospholipase A1 and lipase preparations released fatty acids from both positions of the substrate molecule and as a result glyceryl-phosphoryl-choline was formed.
Both the A2 phospholipase preparations tested produced lysophospholipids through partial selective hydrolysis of phospholipids.
Original languageEnglish
Pages (from-to)393-401
JournalProcess Biochemistry
Volume30
Issue number5
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

Fingerprint

Phospholipases
Phospholipids
Lipases
Lipase
Hydrolysis
Phospholipases A2
Soybeans
Phospholipases A1
Lysophospholipase
Lysophospholipids
Temperature
Aspergillus niger
Phospholipases A
Aspergillus
Penicillium
Isoelectric Point
Choline
Fatty acids
varespladib methyl
Pancreas

Cite this

Mustranta, Annikka ; Forssell, Pirkko ; Poutanen, Kaisa. / Comparison of lipases and phospholipases in the hydrolysis of phospholipids. In: Process Biochemistry. 1995 ; Vol. 30, No. 5. pp. 393-401.
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Comparison of lipases and phospholipases in the hydrolysis of phospholipids. / Mustranta, Annikka (Corresponding Author); Forssell, Pirkko; Poutanen, Kaisa.

In: Process Biochemistry, Vol. 30, No. 5, 1995, p. 393-401.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Comparison of lipases and phospholipases in the hydrolysis of phospholipids

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AU - Poutanen, Kaisa

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