Comparison of lipases and phospholipases in the hydrolysis of phospholipids

Annikka Mustranta (Corresponding Author), Pirkko Forssell, Kaisa Poutanen

    Research output: Contribution to journalArticleScientificpeer-review

    41 Citations (Scopus)

    Abstract

    Two commercial fungal lipase preparations from Aspergillus niger and Penicillium cyclopium, two fungal phospholipases from A. niger (A1 and A2) and one phospholipase from porcine pancreas (A2, Lecitase) were compared in the hydrolysis of soybean phospholipids. The isoelectric points, pH and temperature optima and temperature stabilities of the phospholipases in these enzyme preparations were also determined.
    Lipase preparations which also contained phospholipase and lysophospholipase activities were more efficient than phospholipase preparations in the hydrolysis of soybean phospholipids. Phospholipase A1 and lipase preparations released fatty acids from both positions of the substrate molecule and as a result glyceryl-phosphoryl-choline was formed.
    Both the A2 phospholipase preparations tested produced lysophospholipids through partial selective hydrolysis of phospholipids.
    Original languageEnglish
    Pages (from-to)393-401
    JournalProcess Biochemistry
    Volume30
    Issue number5
    DOIs
    Publication statusPublished - 1995
    MoE publication typeA1 Journal article-refereed

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