Comparison of plant-based expression platforms for the heterologous production of geraniol

N Vasilev (Corresponding Author), C Schmitz, L Dong, Anneli Ritala, N Imseng, Suvi Häkkinen, S van der Krol, R Eibl, Kirsi-Marja Oksman-Caldentey, H Bouwmeester, R Fischer, S Schillberg

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

We compared the ability of different plant-based expression platforms to produce geraniol, a key metabolite in the monoterpenoid branch of the terpenoid indole alkaloid biosynthesis pathway. A geraniol synthase gene isolated from Valeriana officinalis (VoGES) was stably expressed in different tobacco systems. Intact plants were grown in vitro and in the greenhouse and were used to generate cell suspension and hairy root cultures. VoGES was also transiently expressed in N. benthamiana. The highest geraniol content was produced by intact transgenic plants grown in vitro (48 µg/g fresh weight, fw), followed by the transient expression system (27 µg/g fw), transgenic plants under hydroponic conditions in the greenhouse and cell suspension cultures (16 µg/g fw), and finally hairy root cultures (9 µg/g fw). Differences in biomass production and the duration of cultivation resulted in a spectrum of geraniol productivities. Cell suspension cultures achieved a geraniol production rate of 1.8 µg/g fresh biomass per day, whereas transient expression produced 5.9 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is ignored) or 0.5 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is included). The superior productivity, strict process control and simple handling procedures available for transgenic cell suspension cultures suggest that cells are the most promising system for further optimization and ultimately for the scaled-up production of geraniol
Original languageEnglish
Pages (from-to)373-380
JournalPlant Cell, Tissue and Organ Culture
Volume117
Issue number3
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

geraniol
cell suspension culture
agroinfiltration
transgenic plants
biomass
Valeriana officinalis
greenhouses
indole alkaloids
process control
hydroponics
monoterpenoids
terpenoids
biomass production
tobacco
genetically modified organisms
biosynthesis
metabolites
duration
genes

Keywords

  • Cell suspension cultures
  • geraniol synthase
  • hydroponics
  • tobacco plants
  • Valeriana officinalis

Cite this

Vasilev, N ; Schmitz, C ; Dong, L ; Ritala, Anneli ; Imseng, N ; Häkkinen, Suvi ; van der Krol, S ; Eibl, R ; Oksman-Caldentey, Kirsi-Marja ; Bouwmeester, H ; Fischer, R ; Schillberg, S. / Comparison of plant-based expression platforms for the heterologous production of geraniol. In: Plant Cell, Tissue and Organ Culture. 2014 ; Vol. 117, No. 3. pp. 373-380.
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abstract = "We compared the ability of different plant-based expression platforms to produce geraniol, a key metabolite in the monoterpenoid branch of the terpenoid indole alkaloid biosynthesis pathway. A geraniol synthase gene isolated from Valeriana officinalis (VoGES) was stably expressed in different tobacco systems. Intact plants were grown in vitro and in the greenhouse and were used to generate cell suspension and hairy root cultures. VoGES was also transiently expressed in N. benthamiana. The highest geraniol content was produced by intact transgenic plants grown in vitro (48 µg/g fresh weight, fw), followed by the transient expression system (27 µg/g fw), transgenic plants under hydroponic conditions in the greenhouse and cell suspension cultures (16 µg/g fw), and finally hairy root cultures (9 µg/g fw). Differences in biomass production and the duration of cultivation resulted in a spectrum of geraniol productivities. Cell suspension cultures achieved a geraniol production rate of 1.8 µg/g fresh biomass per day, whereas transient expression produced 5.9 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is ignored) or 0.5 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is included). The superior productivity, strict process control and simple handling procedures available for transgenic cell suspension cultures suggest that cells are the most promising system for further optimization and ultimately for the scaled-up production of geraniol",
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Vasilev, N, Schmitz, C, Dong, L, Ritala, A, Imseng, N, Häkkinen, S, van der Krol, S, Eibl, R, Oksman-Caldentey, K-M, Bouwmeester, H, Fischer, R & Schillberg, S 2014, 'Comparison of plant-based expression platforms for the heterologous production of geraniol', Plant Cell, Tissue and Organ Culture, vol. 117, no. 3, pp. 373-380. https://doi.org/10.1007/s11240-014-0446-z

Comparison of plant-based expression platforms for the heterologous production of geraniol. / Vasilev, N (Corresponding Author); Schmitz, C; Dong, L; Ritala, Anneli; Imseng, N; Häkkinen, Suvi; van der Krol, S; Eibl, R; Oksman-Caldentey, Kirsi-Marja; Bouwmeester, H; Fischer, R; Schillberg, S.

In: Plant Cell, Tissue and Organ Culture, Vol. 117, No. 3, 2014, p. 373-380.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Comparison of plant-based expression platforms for the heterologous production of geraniol

AU - Vasilev, N

AU - Schmitz, C

AU - Dong, L

AU - Ritala, Anneli

AU - Imseng, N

AU - Häkkinen, Suvi

AU - van der Krol, S

AU - Eibl, R

AU - Oksman-Caldentey, Kirsi-Marja

AU - Bouwmeester, H

AU - Fischer, R

AU - Schillberg, S

PY - 2014

Y1 - 2014

N2 - We compared the ability of different plant-based expression platforms to produce geraniol, a key metabolite in the monoterpenoid branch of the terpenoid indole alkaloid biosynthesis pathway. A geraniol synthase gene isolated from Valeriana officinalis (VoGES) was stably expressed in different tobacco systems. Intact plants were grown in vitro and in the greenhouse and were used to generate cell suspension and hairy root cultures. VoGES was also transiently expressed in N. benthamiana. The highest geraniol content was produced by intact transgenic plants grown in vitro (48 µg/g fresh weight, fw), followed by the transient expression system (27 µg/g fw), transgenic plants under hydroponic conditions in the greenhouse and cell suspension cultures (16 µg/g fw), and finally hairy root cultures (9 µg/g fw). Differences in biomass production and the duration of cultivation resulted in a spectrum of geraniol productivities. Cell suspension cultures achieved a geraniol production rate of 1.8 µg/g fresh biomass per day, whereas transient expression produced 5.9 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is ignored) or 0.5 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is included). The superior productivity, strict process control and simple handling procedures available for transgenic cell suspension cultures suggest that cells are the most promising system for further optimization and ultimately for the scaled-up production of geraniol

AB - We compared the ability of different plant-based expression platforms to produce geraniol, a key metabolite in the monoterpenoid branch of the terpenoid indole alkaloid biosynthesis pathway. A geraniol synthase gene isolated from Valeriana officinalis (VoGES) was stably expressed in different tobacco systems. Intact plants were grown in vitro and in the greenhouse and were used to generate cell suspension and hairy root cultures. VoGES was also transiently expressed in N. benthamiana. The highest geraniol content was produced by intact transgenic plants grown in vitro (48 µg/g fresh weight, fw), followed by the transient expression system (27 µg/g fw), transgenic plants under hydroponic conditions in the greenhouse and cell suspension cultures (16 µg/g fw), and finally hairy root cultures (9 µg/g fw). Differences in biomass production and the duration of cultivation resulted in a spectrum of geraniol productivities. Cell suspension cultures achieved a geraniol production rate of 1.8 µg/g fresh biomass per day, whereas transient expression produced 5.9 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is ignored) or 0.5 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is included). The superior productivity, strict process control and simple handling procedures available for transgenic cell suspension cultures suggest that cells are the most promising system for further optimization and ultimately for the scaled-up production of geraniol

KW - Cell suspension cultures

KW - geraniol synthase

KW - hydroponics

KW - tobacco plants

KW - Valeriana officinalis

U2 - 10.1007/s11240-014-0446-z

DO - 10.1007/s11240-014-0446-z

M3 - Article

VL - 117

SP - 373

EP - 380

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 3

ER -