Comparison of pretreatments and cost-optimization of enzymatic hydrolysis for production of single cell protein from grass silage fibre

Ville Pihlajaniemi (Corresponding Author), Simo Ellilä, Sakari Poikkimäki, Marja Nappa, Marketta Rinne, Raija Lantto, Matti Siika-aho

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

Grass silage is a promising biorefinery feedstock with surplus production potential, providing a source of readily soluble protein and lignocellulosic fibre. This study presents a concept combining protein extraction with production of single cell protein from enzymatically saccharified grass silage fibre by fermentation of the filamentous fungus Paecilomyces variotii. Steam explosion and ammonia soaking were compared as pretreatments, leading to 81.2% and 88.1% carbohydrate hydrolysability, respectively. Microbial biomass yields of 51% from hydrolysate sugars were reached with a protein content of 51% of cell dry weight. A single-reactor ammonia pretreatment and hydrolysis process was demonstrated, including ammonia recovery of up to 66%, while the residual ammonia was synergistically utilized as a nitrogen source for protein production. The effect of cellulase dosage, hydrolysis time and solids concentration was empirically modelled and the model was applied for cost optimization of enzymatic hydrolysis as a part of a techno-economic assessment of the process.
Original languageEnglish
Article number100357
JournalBioresource Technology Reports
Volume9
DOIs
Publication statusPublished - Feb 2020
MoE publication typeA1 Journal article-refereed

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single cell protein
grass silage
enzymatic hydrolysis
dietary fiber
ammonia
pretreatment
hydrolysis
biorefining
explosions
feedstocks
surpluses
hydrolysates
soaking
endo-1,4-beta-glucanase
steam
protein sources
microbial biomass
proteins
fermentation
protein content

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Pihlajaniemi, Ville ; Ellilä, Simo ; Poikkimäki, Sakari ; Nappa, Marja ; Rinne, Marketta ; Lantto, Raija ; Siika-aho, Matti. / Comparison of pretreatments and cost-optimization of enzymatic hydrolysis for production of single cell protein from grass silage fibre. In: Bioresource Technology Reports. 2020 ; Vol. 9.
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abstract = "Grass silage is a promising biorefinery feedstock with surplus production potential, providing a source of readily soluble protein and lignocellulosic fibre. This study presents a concept combining protein extraction with production of single cell protein from enzymatically saccharified grass silage fibre by fermentation of the filamentous fungus Paecilomyces variotii. Steam explosion and ammonia soaking were compared as pretreatments, leading to 81.2{\%} and 88.1{\%} carbohydrate hydrolysability, respectively. Microbial biomass yields of 51{\%} from hydrolysate sugars were reached with a protein content of 51{\%} of cell dry weight. A single-reactor ammonia pretreatment and hydrolysis process was demonstrated, including ammonia recovery of up to 66{\%}, while the residual ammonia was synergistically utilized as a nitrogen source for protein production. The effect of cellulase dosage, hydrolysis time and solids concentration was empirically modelled and the model was applied for cost optimization of enzymatic hydrolysis as a part of a techno-economic assessment of the process.",
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Pihlajaniemi, V, Ellilä, S, Poikkimäki, S, Nappa, M, Rinne, M, Lantto, R & Siika-aho, M 2020, 'Comparison of pretreatments and cost-optimization of enzymatic hydrolysis for production of single cell protein from grass silage fibre', Bioresource Technology Reports, vol. 9, 100357. https://doi.org/10.1016/j.biteb.2019.100357

Comparison of pretreatments and cost-optimization of enzymatic hydrolysis for production of single cell protein from grass silage fibre. / Pihlajaniemi, Ville (Corresponding Author); Ellilä, Simo; Poikkimäki, Sakari; Nappa, Marja; Rinne, Marketta; Lantto, Raija; Siika-aho, Matti.

In: Bioresource Technology Reports, Vol. 9, 100357, 02.2020.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

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AU - Pihlajaniemi, Ville

AU - Ellilä, Simo

AU - Poikkimäki, Sakari

AU - Nappa, Marja

AU - Rinne, Marketta

AU - Lantto, Raija

AU - Siika-aho, Matti

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N2 - Grass silage is a promising biorefinery feedstock with surplus production potential, providing a source of readily soluble protein and lignocellulosic fibre. This study presents a concept combining protein extraction with production of single cell protein from enzymatically saccharified grass silage fibre by fermentation of the filamentous fungus Paecilomyces variotii. Steam explosion and ammonia soaking were compared as pretreatments, leading to 81.2% and 88.1% carbohydrate hydrolysability, respectively. Microbial biomass yields of 51% from hydrolysate sugars were reached with a protein content of 51% of cell dry weight. A single-reactor ammonia pretreatment and hydrolysis process was demonstrated, including ammonia recovery of up to 66%, while the residual ammonia was synergistically utilized as a nitrogen source for protein production. The effect of cellulase dosage, hydrolysis time and solids concentration was empirically modelled and the model was applied for cost optimization of enzymatic hydrolysis as a part of a techno-economic assessment of the process.

AB - Grass silage is a promising biorefinery feedstock with surplus production potential, providing a source of readily soluble protein and lignocellulosic fibre. This study presents a concept combining protein extraction with production of single cell protein from enzymatically saccharified grass silage fibre by fermentation of the filamentous fungus Paecilomyces variotii. Steam explosion and ammonia soaking were compared as pretreatments, leading to 81.2% and 88.1% carbohydrate hydrolysability, respectively. Microbial biomass yields of 51% from hydrolysate sugars were reached with a protein content of 51% of cell dry weight. A single-reactor ammonia pretreatment and hydrolysis process was demonstrated, including ammonia recovery of up to 66%, while the residual ammonia was synergistically utilized as a nitrogen source for protein production. The effect of cellulase dosage, hydrolysis time and solids concentration was empirically modelled and the model was applied for cost optimization of enzymatic hydrolysis as a part of a techno-economic assessment of the process.

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Pihlajaniemi V, Ellilä S, Poikkimäki S, Nappa M, Rinne M, Lantto R et al. Comparison of pretreatments and cost-optimization of enzymatic hydrolysis for production of single cell protein from grass silage fibre. Bioresource Technology Reports. 2020 Feb;9. 100357. https://doi.org/10.1016/j.biteb.2019.100357

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