TY - JOUR
T1 - Comparison of pretreatments and cost-optimization of enzymatic hydrolysis for production of single cell protein from grass silage fibre
AU - Pihlajaniemi, Ville
AU - Ellilä, Simo
AU - Poikkimäki, Sakari
AU - Nappa, Marja
AU - Rinne, Marketta
AU - Lantto, Raija
AU - Siika-aho, Matti
N1 - Funding Information:
We gratefully acknowledge Business Finland (The Innofeed project) for funding of this work.
PY - 2020/2
Y1 - 2020/2
N2 - Grass silage is a promising biorefinery feedstock with surplus production potential, providing a source of readily soluble protein and lignocellulosic fibre. This study presents a concept combining protein extraction with production of single cell protein from enzymatically saccharified grass silage fibre by fermentation of the filamentous fungus Paecilomyces variotii. Steam explosion and ammonia soaking were compared as pretreatments, leading to 81.2% and 88.1% carbohydrate hydrolysability, respectively. Microbial biomass yields of 51% from hydrolysate sugars were reached with a protein content of 51% of cell dry weight. A single-reactor ammonia pretreatment and hydrolysis process was demonstrated, including ammonia recovery of up to 66%, while the residual ammonia was synergistically utilized as a nitrogen source for protein production. The effect of cellulase dosage, hydrolysis time and solids concentration was empirically modelled and the model was applied for cost optimization of enzymatic hydrolysis as a part of a techno-economic assessment of the process.
AB - Grass silage is a promising biorefinery feedstock with surplus production potential, providing a source of readily soluble protein and lignocellulosic fibre. This study presents a concept combining protein extraction with production of single cell protein from enzymatically saccharified grass silage fibre by fermentation of the filamentous fungus Paecilomyces variotii. Steam explosion and ammonia soaking were compared as pretreatments, leading to 81.2% and 88.1% carbohydrate hydrolysability, respectively. Microbial biomass yields of 51% from hydrolysate sugars were reached with a protein content of 51% of cell dry weight. A single-reactor ammonia pretreatment and hydrolysis process was demonstrated, including ammonia recovery of up to 66%, while the residual ammonia was synergistically utilized as a nitrogen source for protein production. The effect of cellulase dosage, hydrolysis time and solids concentration was empirically modelled and the model was applied for cost optimization of enzymatic hydrolysis as a part of a techno-economic assessment of the process.
UR - http://www.scopus.com/inward/record.url?scp=85076096036&partnerID=8YFLogxK
U2 - 10.1016/j.biteb.2019.100357
DO - 10.1016/j.biteb.2019.100357
M3 - Article
VL - 9
JO - Bioresource Technology Reports
JF - Bioresource Technology Reports
SN - 2589-014X
M1 - 100357
ER -