Comparison of Pseudomonas fragi and Gluconobacter oxydans for production of xylonic acid from hemicellulose hydrolyzates

Johanna Buchert, Jurgen Puls, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

Xylonic acid was produced efficiently from pure xylose by Pseudomonas fragi ATCC 4973 and Gluconobacter oxydans subsp. suboxydans ATCC 621. The yield from 10% xylose was in both cases over 95% of the theoretical.
However, the sensitivities of the strains towards the major inhibitors found in hemicellulose hydrolyzates, ie. acetic acid, furfural and two lignin-derived compounds, varied. G. oxydans tolerated all these inhibitors better than P. fragi. In tests using steamed hemicellulose hydrolyzate, G. oxydans was able to utilize the substrate only at dilute xylose concentrations.
After ether extraction or mixed bed resin pretreatment, the fermentability of the hydrolyzate was increased significantly.
Original languageEnglish
Pages (from-to)367-372
JournalApplied Microbiology and Biotechnology
Volume28
Issue number4-5
DOIs
Publication statusPublished - 1988
MoE publication typeA1 Journal article-refereed

Fingerprint

Pseudomonas fragi
Gluconobacter oxydans
Xylose
Acids
Furaldehyde
Lignin
Acetic Acid
Ether
hemicellulose

Cite this

@article{87eca86767704a9294509f5bdaf47022,
title = "Comparison of Pseudomonas fragi and Gluconobacter oxydans for production of xylonic acid from hemicellulose hydrolyzates",
abstract = "Xylonic acid was produced efficiently from pure xylose by Pseudomonas fragi ATCC 4973 and Gluconobacter oxydans subsp. suboxydans ATCC 621. The yield from 10{\%} xylose was in both cases over 95{\%} of the theoretical. However, the sensitivities of the strains towards the major inhibitors found in hemicellulose hydrolyzates, ie. acetic acid, furfural and two lignin-derived compounds, varied. G. oxydans tolerated all these inhibitors better than P. fragi. In tests using steamed hemicellulose hydrolyzate, G. oxydans was able to utilize the substrate only at dilute xylose concentrations. After ether extraction or mixed bed resin pretreatment, the fermentability of the hydrolyzate was increased significantly.",
author = "Johanna Buchert and Jurgen Puls and Kaisa Poutanen",
year = "1988",
doi = "10.1007/BF00268197",
language = "English",
volume = "28",
pages = "367--372",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer",
number = "4-5",

}

Comparison of Pseudomonas fragi and Gluconobacter oxydans for production of xylonic acid from hemicellulose hydrolyzates. / Buchert, Johanna; Puls, Jurgen; Poutanen, Kaisa.

In: Applied Microbiology and Biotechnology, Vol. 28, No. 4-5, 1988, p. 367-372.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Comparison of Pseudomonas fragi and Gluconobacter oxydans for production of xylonic acid from hemicellulose hydrolyzates

AU - Buchert, Johanna

AU - Puls, Jurgen

AU - Poutanen, Kaisa

PY - 1988

Y1 - 1988

N2 - Xylonic acid was produced efficiently from pure xylose by Pseudomonas fragi ATCC 4973 and Gluconobacter oxydans subsp. suboxydans ATCC 621. The yield from 10% xylose was in both cases over 95% of the theoretical. However, the sensitivities of the strains towards the major inhibitors found in hemicellulose hydrolyzates, ie. acetic acid, furfural and two lignin-derived compounds, varied. G. oxydans tolerated all these inhibitors better than P. fragi. In tests using steamed hemicellulose hydrolyzate, G. oxydans was able to utilize the substrate only at dilute xylose concentrations. After ether extraction or mixed bed resin pretreatment, the fermentability of the hydrolyzate was increased significantly.

AB - Xylonic acid was produced efficiently from pure xylose by Pseudomonas fragi ATCC 4973 and Gluconobacter oxydans subsp. suboxydans ATCC 621. The yield from 10% xylose was in both cases over 95% of the theoretical. However, the sensitivities of the strains towards the major inhibitors found in hemicellulose hydrolyzates, ie. acetic acid, furfural and two lignin-derived compounds, varied. G. oxydans tolerated all these inhibitors better than P. fragi. In tests using steamed hemicellulose hydrolyzate, G. oxydans was able to utilize the substrate only at dilute xylose concentrations. After ether extraction or mixed bed resin pretreatment, the fermentability of the hydrolyzate was increased significantly.

U2 - 10.1007/BF00268197

DO - 10.1007/BF00268197

M3 - Article

VL - 28

SP - 367

EP - 372

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 4-5

ER -