Comparison of Pseudomonas fragi and Gluconobacter oxydans for production of xylonic acid from hemicellulose hydrolyzates

Johanna Buchert, Jurgen Puls, Kaisa Poutanen

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Xylonic acid was produced efficiently from pure xylose by Pseudomonas fragi ATCC 4973 and Gluconobacter oxydans subsp. suboxydans ATCC 621. The yield from 10% xylose was in both cases over 95% of the theoretical.
However, the sensitivities of the strains towards the major inhibitors found in hemicellulose hydrolyzates, ie. acetic acid, furfural and two lignin-derived compounds, varied. G. oxydans tolerated all these inhibitors better than P. fragi. In tests using steamed hemicellulose hydrolyzate, G. oxydans was able to utilize the substrate only at dilute xylose concentrations.
After ether extraction or mixed bed resin pretreatment, the fermentability of the hydrolyzate was increased significantly.
Original languageEnglish
Pages (from-to)367-372
JournalApplied Microbiology and Biotechnology
Issue number4-5
Publication statusPublished - 1988
MoE publication typeA1 Journal article-refereed


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