Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli

Jussi Holmalahti, Harri Santa, Hartmut Laatsch, Atte von Wright

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Abstract

We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.

Original languageEnglish
Pages (from-to)33-38
JournalMutation Research: Fundamental and molecular mechanisms of mutagenesis
Volume391
Issue number1-2
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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