Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli

Jussi Holmalahti, Harri Santa, Hartmut Laatsch, Atte von Wright

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.

Original languageEnglish
Pages (from-to)33 - 38
Number of pages6
JournalMutation Research: Fundamental and molecular mechanisms of mutagenesis
Volume391
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Dactinomycin
Escherichia coli
Anti-Bacterial Agents
Streptomyces
Microsomes
Salmonella
Biological Assay
Culture Media
Databases

Cite this

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title = "Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli",
abstract = "We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.",
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pages = "33 -- 38",
journal = "Mutation Research: Fundamental and molecular mechanisms of mutagenesis",
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Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli. / Holmalahti, Jussi; Santa, Harri; Laatsch, Hartmut; von Wright, Atte.

In: Mutation Research: Fundamental and molecular mechanisms of mutagenesis, Vol. 391, 1997, p. 33 - 38.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli

AU - Holmalahti, Jussi

AU - Santa, Harri

AU - Laatsch, Hartmut

AU - von Wright, Atte

PY - 1997

Y1 - 1997

N2 - We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.

AB - We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.

M3 - Article

VL - 391

SP - 33

EP - 38

JO - Mutation Research: Fundamental and molecular mechanisms of mutagenesis

JF - Mutation Research: Fundamental and molecular mechanisms of mutagenesis

SN - 0027-5107

ER -