Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli

Jussi Holmalahti; Harri Santa; Hartmut Laatsch; Atte von Wright

doi:10.1016/s0165-1218(97)00023-2

Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli

Jussi Holmalahti, Harri Santa, Hartmut Laatsch, Atte von Wright

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.

Original language	English
Pages (from-to)	33-38
Journal	Mutation Research: Fundamental and molecular mechanisms of mutagenesis
Volume	391
Issue number	1-2
DOIs	https://doi.org/10.1016/s0165-1218(97)00023-2
Publication status	Published - 1997
MoE publication type	A1 Journal article-refereed

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title = "Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli",

abstract = "We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.",

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T1 - Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli

AU - Holmalahti, Jussi

AU - Santa, Harri

AU - Laatsch, Hartmut

AU - von Wright, Atte

PY - 1997

Y1 - 1997

N2 - We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.

AB - We report here the isolation and purification of a genotoxic antibiotic from the culture medium of a Streptomyces strain. The antibiotic was identified as actinomycin X2 by using a database search (AntiBase). In the previous studies a related compound, actinomycin D, has been shown to be non-mutagenic in the salmonella/microsome assay and several other bacterial systems. The fact that an actinomycin was detected by a bacterial repair assay seems to contradict these results. Therefore we tested several actinomycins by differential killing assay based on the same Escherichia coli strains that were used in our screening bioassays. According to our results the uvrA, recA double mutant sensitizes E. coli to the genotoxic effects of actinomycins.

U2 - 10.1016/s0165-1218(97)00023-2

DO - 10.1016/s0165-1218(97)00023-2

M3 - Article

SN - 0027-5107

VL - 391

SP - 33

EP - 38

JO - Mutation Research: Fundamental and molecular mechanisms of mutagenesis

JF - Mutation Research: Fundamental and molecular mechanisms of mutagenesis

IS - 1-2

ER -

Comparison of the lethal effects of different actinomycins on a repair-deficient strain of Escherichia coli

Abstract

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