Comparison of three test media for antimicrobial susceptibility testing of bifidobacteria using the Etest method

Jaana Mättö (Corresponding Author), Maija-Liisa Suihko, Maria Saarela

    Research output: Contribution to journalArticleScientificpeer-review

    22 Citations (Scopus)

    Abstract

    The performance of three test media for antimicrobial susceptibility testing of bifidobacteria using the Etest was compared. All Bifidobacterium strains (n = 42) displayed good growth on trypticase–phytone–yeast extract agar (TPY). Most strains showed good growth on lactic acid bacteria susceptibility test medium supplemented with cysteine (LSM + cys); Bifidobacterium bifidum showed moderate growth. Growth of seven strains was inadequate on Brucella blood agar (BRU) and an additional eight strains showed moderate growth. The minimum inhibitory concentrations (MICs) for tetracycline were highest on BRU and lowest on LSM + cys (agreement 57%), whereas the MICs for streptomycin were lowest on BRU and highest on TPY (agreement 40%). Occasional mismatches (agreement 71–91%) between the test media were also detected for the β-lactam antibiotics. This study describes test medium-dependent variation of MICs and the applicability of LSM + cys for antimicrobial susceptibility testing of bifidobacteria.
    Original languageEnglish
    Pages (from-to)42-48
    Number of pages7
    JournalInternational Journal of Antimicrobial Agents
    Volume28
    Issue number1
    DOIs
    Publication statusPublished - 2006
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Bifidobacterium
    • Antimicrobial susceptibility
    • Test medium
    • Etest

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