Comparison of three test media for antimicrobial susceptibility testing of bifidobacteria using the Etest method

Jaana Mättö (Corresponding Author), Maija-Liisa Suihko, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

The performance of three test media for antimicrobial susceptibility testing of bifidobacteria using the Etest was compared. All Bifidobacterium strains (n = 42) displayed good growth on trypticase–phytone–yeast extract agar (TPY). Most strains showed good growth on lactic acid bacteria susceptibility test medium supplemented with cysteine (LSM + cys); Bifidobacterium bifidum showed moderate growth. Growth of seven strains was inadequate on Brucella blood agar (BRU) and an additional eight strains showed moderate growth. The minimum inhibitory concentrations (MICs) for tetracycline were highest on BRU and lowest on LSM + cys (agreement 57%), whereas the MICs for streptomycin were lowest on BRU and highest on TPY (agreement 40%). Occasional mismatches (agreement 71–91%) between the test media were also detected for the β-lactam antibiotics. This study describes test medium-dependent variation of MICs and the applicability of LSM + cys for antimicrobial susceptibility testing of bifidobacteria.
Original languageEnglish
Pages (from-to)42-48
Number of pages7
JournalInternational Journal of Antimicrobial Agents
Volume28
Issue number1
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

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Disk Diffusion Antimicrobial Tests
Bifidobacterium
Microbial Sensitivity Tests
Growth
Agar
Lactams
Brucella
Streptomycin
Tetracycline
Cysteine
Lactic Acid
Anti-Bacterial Agents
Bacteria

Keywords

  • Bifidobacterium
  • Antimicrobial susceptibility
  • Test medium
  • Etest

Cite this

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title = "Comparison of three test media for antimicrobial susceptibility testing of bifidobacteria using the Etest method",
abstract = "The performance of three test media for antimicrobial susceptibility testing of bifidobacteria using the Etest was compared. All Bifidobacterium strains (n = 42) displayed good growth on trypticase–phytone–yeast extract agar (TPY). Most strains showed good growth on lactic acid bacteria susceptibility test medium supplemented with cysteine (LSM + cys); Bifidobacterium bifidum showed moderate growth. Growth of seven strains was inadequate on Brucella blood agar (BRU) and an additional eight strains showed moderate growth. The minimum inhibitory concentrations (MICs) for tetracycline were highest on BRU and lowest on LSM + cys (agreement 57{\%}), whereas the MICs for streptomycin were lowest on BRU and highest on TPY (agreement 40{\%}). Occasional mismatches (agreement 71–91{\%}) between the test media were also detected for the β-lactam antibiotics. This study describes test medium-dependent variation of MICs and the applicability of LSM + cys for antimicrobial susceptibility testing of bifidobacteria.",
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Comparison of three test media for antimicrobial susceptibility testing of bifidobacteria using the Etest method. / Mättö, Jaana (Corresponding Author); Suihko, Maija-Liisa; Saarela, Maria.

In: International Journal of Antimicrobial Agents, Vol. 28, No. 1, 2006, p. 42-48.

Research output: Contribution to journalArticleScientificpeer-review

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