TY - JOUR
T1 - Comparison of virulence factors of oral Candida dubliniensis and Candida albicans isolates in healthy people and patients with chronic candidosis
AU - Hannula, J.
AU - Saarela, Maria
AU - Dogan, B.
AU - Paatsama, J.
AU - Koukila-Kähkölä, P.
AU - Pirinen, S.
AU - Alakomi, Hanna-Leena
AU - Perheentupa, J.
AU - Asikainen, S.
PY - 2000
Y1 - 2000
N2 - We determined differences in the expression of certain virulence factors between oral Candida dubliniensis and Candida albicans species. In addition, clonal differences were sought among C. albicans
isolates recovered from patients with and without compromised immune
system. The material comprised 93 clinical yeast isolates originated in
40 subjects (1–5 isolates per subject). All 26 C. dubliniensis isolates and 46 C. albicans isolates originated from healthy routine dental clinic patients. Additionally, 21 C. albicans
isolates were collected from patients with autoimmune
polyendocrinopathy–candidosis–ectodermal dystrophy (APECED), who have
chronic candidosis as one manifestation of their immunocompromising
disease. Polymerase chain reaction amplification using the random
sequence primer OPE‐03 enabled grouping of the C. dubliniensis isolates in 2 genotypes (I and II) and C. albicans
isolates in 15 genotypes (I–XV). No significant difference was found in
the distribution of genotypes between the patients with APECED and the
healthy subjects. C. dubliniensis isolates exhibited high‐frequency phenotypic switching significantly more frequently than did C. albicans
isolates, and vice versa regarding phospholipase and proteinase
production. Proteinase production was significantly more frequent among C. albicans genotype V than genotype IX isolates. No significant difference was found in expression of virulence factors of C. albicans isolates between the patients with APECED and the healthy subjects.
AB - We determined differences in the expression of certain virulence factors between oral Candida dubliniensis and Candida albicans species. In addition, clonal differences were sought among C. albicans
isolates recovered from patients with and without compromised immune
system. The material comprised 93 clinical yeast isolates originated in
40 subjects (1–5 isolates per subject). All 26 C. dubliniensis isolates and 46 C. albicans isolates originated from healthy routine dental clinic patients. Additionally, 21 C. albicans
isolates were collected from patients with autoimmune
polyendocrinopathy–candidosis–ectodermal dystrophy (APECED), who have
chronic candidosis as one manifestation of their immunocompromising
disease. Polymerase chain reaction amplification using the random
sequence primer OPE‐03 enabled grouping of the C. dubliniensis isolates in 2 genotypes (I and II) and C. albicans
isolates in 15 genotypes (I–XV). No significant difference was found in
the distribution of genotypes between the patients with APECED and the
healthy subjects. C. dubliniensis isolates exhibited high‐frequency phenotypic switching significantly more frequently than did C. albicans
isolates, and vice versa regarding phospholipase and proteinase
production. Proteinase production was significantly more frequent among C. albicans genotype V than genotype IX isolates. No significant difference was found in expression of virulence factors of C. albicans isolates between the patients with APECED and the healthy subjects.
U2 - 10.1034/j.1399-302x.2000.150405.x
DO - 10.1034/j.1399-302x.2000.150405.x
M3 - Article
SN - 2041-1006
VL - 15
SP - 238
EP - 244
JO - Molecular Oral Microbiology
JF - Molecular Oral Microbiology
IS - 4
ER -