Complexes of Magnetic Nanoparticles with Cellulose Nanocrystals as Regenerable, Highly Efficient, and Selective Platform for Protein Separation

Guo Jiaqi; Ilari Filpponen; Leena-Sisko Johansson; Pezhman Mohammadi; Mika Latikka; Markus Linder; Robin H.A. Ras; Orlando, J. Rojas

doi:10.1021/acs.biomac.6b01778

Complexes of Magnetic Nanoparticles with Cellulose Nanocrystals as Regenerable, Highly Efficient, and Selective Platform for Protein Separation

Guo Jiaqi, Ilari Filpponen, Leena-Sisko Johansson, Pezhman Mohammadi, Mika Latikka, Markus Linder, Robin H.A. Ras, Orlando, J. Rojas

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55 Citations (Scopus)

Abstract

We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive Fe ₃O ₄ nanoparticles that were synthesized using the (Fe ³⁺/Fe ²⁺) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupling amine-containing iron oxide nanoparticles that were achieved by dopamine ligand exchange (NH ₂-Fe ₃O ₄ NPs). The as-prepared hybrids (Fe ₃O ₄@CNC) were further complexed with Cu(II) ions to produce specific protein binding sites. The performance of magnetically responsive Cu-Fe ₃O ₄@CNC hybrids was assessed by selectively separating lysozyme from aqueous media. The hybrid system displayed a remarkable binding capacity with lysozyme of 860.6 ± 14.6 mg/g while near full protein recovery (∼98%) was achieved by simple elution. Moreover, the regeneration of Fe ₃O ₄@CNC hybrids and efficient reutilization for protein separation was demonstrated. Finally, lysozyme separation from matrices containing egg white was achieved, thus revealing the specificity and potential of the presented method.

Original language	English
Pages (from-to)	898–905
Number of pages	8
Journal	Biomacromolecules
Volume	18
Issue number	3
DOIs	https://doi.org/10.1021/acs.biomac.6b01778
Publication status	Published - 2017
MoE publication type	A1 Journal article-refereed

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@article{6fd0c2784ade4d15a1c544220273ea8b,

title = "Complexes of Magnetic Nanoparticles with Cellulose Nanocrystals as Regenerable, Highly Efficient, and Selective Platform for Protein Separation",

abstract = "We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive Fe 3O 4 nanoparticles that were synthesized using the (Fe 3+/Fe 2+) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupling amine-containing iron oxide nanoparticles that were achieved by dopamine ligand exchange (NH 2-Fe 3O 4 NPs). The as-prepared hybrids (Fe 3O 4@CNC) were further complexed with Cu(II) ions to produce specific protein binding sites. The performance of magnetically responsive Cu-Fe 3O 4@CNC hybrids was assessed by selectively separating lysozyme from aqueous media. The hybrid system displayed a remarkable binding capacity with lysozyme of 860.6 ± 14.6 mg/g while near full protein recovery (∼98%) was achieved by simple elution. Moreover, the regeneration of Fe 3O 4@CNC hybrids and efficient reutilization for protein separation was demonstrated. Finally, lysozyme separation from matrices containing egg white was achieved, thus revealing the specificity and potential of the presented method. ",

author = "Guo Jiaqi and Ilari Filpponen and Leena-Sisko Johansson and Pezhman Mohammadi and Mika Latikka and Markus Linder and Ras, {Robin H.A.} and Rojas, {Orlando, J.}",

year = "2017",

doi = "10.1021/acs.biomac.6b01778",

language = "English",

volume = "18",

pages = "898–905",

journal = "Biomacromolecules",

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T1 - Complexes of Magnetic Nanoparticles with Cellulose Nanocrystals as Regenerable, Highly Efficient, and Selective Platform for Protein Separation

AU - Jiaqi, Guo

AU - Filpponen, Ilari

AU - Johansson, Leena-Sisko

AU - Mohammadi, Pezhman

AU - Latikka, Mika

AU - Linder, Markus

AU - Ras, Robin H.A.

AU - Rojas, Orlando, J.

PY - 2017

Y1 - 2017

N2 - We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive Fe 3O 4 nanoparticles that were synthesized using the (Fe 3+/Fe 2+) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupling amine-containing iron oxide nanoparticles that were achieved by dopamine ligand exchange (NH 2-Fe 3O 4 NPs). The as-prepared hybrids (Fe 3O 4@CNC) were further complexed with Cu(II) ions to produce specific protein binding sites. The performance of magnetically responsive Cu-Fe 3O 4@CNC hybrids was assessed by selectively separating lysozyme from aqueous media. The hybrid system displayed a remarkable binding capacity with lysozyme of 860.6 ± 14.6 mg/g while near full protein recovery (∼98%) was achieved by simple elution. Moreover, the regeneration of Fe 3O 4@CNC hybrids and efficient reutilization for protein separation was demonstrated. Finally, lysozyme separation from matrices containing egg white was achieved, thus revealing the specificity and potential of the presented method.

AB - We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive Fe 3O 4 nanoparticles that were synthesized using the (Fe 3+/Fe 2+) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupling amine-containing iron oxide nanoparticles that were achieved by dopamine ligand exchange (NH 2-Fe 3O 4 NPs). The as-prepared hybrids (Fe 3O 4@CNC) were further complexed with Cu(II) ions to produce specific protein binding sites. The performance of magnetically responsive Cu-Fe 3O 4@CNC hybrids was assessed by selectively separating lysozyme from aqueous media. The hybrid system displayed a remarkable binding capacity with lysozyme of 860.6 ± 14.6 mg/g while near full protein recovery (∼98%) was achieved by simple elution. Moreover, the regeneration of Fe 3O 4@CNC hybrids and efficient reutilization for protein separation was demonstrated. Finally, lysozyme separation from matrices containing egg white was achieved, thus revealing the specificity and potential of the presented method.

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U2 - 10.1021/acs.biomac.6b01778

DO - 10.1021/acs.biomac.6b01778

M3 - Article

SN - 1525-7797

VL - 18

SP - 898

EP - 905

JO - Biomacromolecules

JF - Biomacromolecules

IS - 3

ER -

Complexes of Magnetic Nanoparticles with Cellulose Nanocrystals as Regenerable, Highly Efficient, and Selective Platform for Protein Separation

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