Composition and temporal stability of gastrointestinal microbiota in irritable bowel syndrome

A longitudinal study in IBS and control subjects

Jaana Mättö (Corresponding Author), Liisa Maunuksela, Kajsa Kajander, Airi Palva, Riitta Korpela, Anna Kassinen, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

215 Citations (Scopus)

Abstract

Irritable bowel syndrome (IBS) is a common intestinal disorder that includes continuous or recurrent intestinal pain and discomfort and altered bowel habits. The pathophysiology of IBS is incompletely understood, but it may involve an altered intestinal microbiota. The aim of the present study was to compare the composition and temporal stability of faecal microbiota of IBS patients and healthy controls by applying culture-based techniques and PCR-DGGE analysis. No difference in the prevalence or mean culturable numbers of bacteroides, bifidobacteria, spore-forming bacteria, lactobacilli, enterococci or yeasts were observed between the IBS and control groups, whereas slightly higher numbers of coliforms as well as an increased aerobe:anaerobe ratio was observed in the IBS group. PCR-DGGE revealed more temporal instability in the predominant bacterial population of IBS subjects than in controls. In 9 out of 21 IBS subjects and 5 out of 17 controls the PCR-DGGE profiles obtained from the samples of the same individual on different occasions (sampling points 0, 3 and 6 months) were clearly different. However, the instability in some of the IBS subjects could partly be explained by antibiotic consumption during the study. The present study suggests that instability of intestinal microbiota may be involved in IBS. However, further studies are needed to associate the instability with specific IBS symptoms or with specific bacterial groups and species.
Original languageEnglish
Pages (from-to)213 - 222
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume43
Issue number2
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Fingerprint

Irritable Bowel Syndrome
Longitudinal Studies
Polymerase Chain Reaction
Gastrointestinal Microbiome
Culture Techniques
Bacteroides
Bifidobacterium
Microbiota
Enterococcus
Lactobacillus
Spores
Habits
Yeasts
Anti-Bacterial Agents
Bacteria
Pain
Control Groups

Keywords

  • Irritable bowel syndrome
  • PCR-DGGE
  • Faecal
  • Intestinal microbiota

Cite this

Mättö, Jaana ; Maunuksela, Liisa ; Kajander, Kajsa ; Palva, Airi ; Korpela, Riitta ; Kassinen, Anna ; Saarela, Maria. / Composition and temporal stability of gastrointestinal microbiota in irritable bowel syndrome : A longitudinal study in IBS and control subjects. In: FEMS Immunology and Medical Microbiology. 2005 ; Vol. 43, No. 2. pp. 213 - 222.
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Composition and temporal stability of gastrointestinal microbiota in irritable bowel syndrome : A longitudinal study in IBS and control subjects. / Mättö, Jaana (Corresponding Author); Maunuksela, Liisa; Kajander, Kajsa; Palva, Airi; Korpela, Riitta; Kassinen, Anna; Saarela, Maria.

In: FEMS Immunology and Medical Microbiology, Vol. 43, No. 2, 2005, p. 213 - 222.

Research output: Contribution to journalArticleScientificpeer-review

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