Composition of bifidobacterial population in irritable bowel syndrome and control subjects

Jaana Mättö, Maria Saarela

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Irritable bowel syndrome (IBS) is a chronic intestinal disorder that may involve an altered intestinal microbiota. Although in some studies lower numbers of bifidobacteria have been detected in IBS subjects compared to healthy controls, the composition of bifidobacterial population in IBS subjects is poorly known. The aim of the present study was to investigate the diversity and stability of faecal bifidobacterial population in IBS and control subjects. Faecal samples were obtained from 19 IBS and 16 control subjects at three sampling points (0, 3 and 6 months) and analysed by PCR-DGGE with Bifidobacterium-specific primers Bifl 64-f and Bif662-GC-r. In addition fresh samples from two sampling occasions were cultured on Beerens to determine the bifidobacterial counts. PCR-DGGE analysis revealed 1-7 fragments per sample. No difference in the bifidobacterial count or in the diversity or stability of bifidobacterial population in general was observed between IBS and control groups. However, a single fragment, identified by 16S rDNA sequencing tentatively as uncultured Bifidobacterium, was more commonly detected in IBS than in control subjects. The identity and role of this fragment in IBS warrants further studies.
Original languageEnglish
Title of host publicationThe Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop
Subtitle of host publicationAbstracts and posters
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages83-83
ISBN (Electronic)951-38-6290-9
ISBN (Print)951-38-6289-5
Publication statusPublished - 2004
EventThe Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop - Sitges, Spain
Duration: 15 Mar 200417 Mar 2004

Publication series

SeriesVTT Symposium
Number232
ISSN0357-9387

Workshop

WorkshopThe Food, GI-tract Functionality and Human Health Cluster
CountrySpain
CitySitges
Period15/03/0417/03/04

Fingerprint

Irritable Bowel Syndrome
Population
Bifidobacterium
Polymerase Chain Reaction
Ribosomal DNA
Control Groups

Cite this

Mättö, J., & Saarela, M. (2004). Composition of bifidobacterial population in irritable bowel syndrome and control subjects. In The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop: Abstracts and posters (pp. 83-83). Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 232
Mättö, Jaana ; Saarela, Maria. / Composition of bifidobacterial population in irritable bowel syndrome and control subjects. The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop: Abstracts and posters. Espoo : VTT Technical Research Centre of Finland, 2004. pp. 83-83 (VTT Symposium; No. 232).
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Mättö, J & Saarela, M 2004, Composition of bifidobacterial population in irritable bowel syndrome and control subjects. in The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop: Abstracts and posters. VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 232, pp. 83-83, The Food, GI-tract Functionality and Human Health Cluster, Sitges, Spain, 15/03/04.

Composition of bifidobacterial population in irritable bowel syndrome and control subjects. / Mättö, Jaana; Saarela, Maria.

The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop: Abstracts and posters. Espoo : VTT Technical Research Centre of Finland, 2004. p. 83-83 (VTT Symposium; No. 232).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Composition of bifidobacterial population in irritable bowel syndrome and control subjects

AU - Mättö, Jaana

AU - Saarela, Maria

PY - 2004

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N2 - Irritable bowel syndrome (IBS) is a chronic intestinal disorder that may involve an altered intestinal microbiota. Although in some studies lower numbers of bifidobacteria have been detected in IBS subjects compared to healthy controls, the composition of bifidobacterial population in IBS subjects is poorly known. The aim of the present study was to investigate the diversity and stability of faecal bifidobacterial population in IBS and control subjects. Faecal samples were obtained from 19 IBS and 16 control subjects at three sampling points (0, 3 and 6 months) and analysed by PCR-DGGE with Bifidobacterium-specific primers Bifl 64-f and Bif662-GC-r. In addition fresh samples from two sampling occasions were cultured on Beerens to determine the bifidobacterial counts. PCR-DGGE analysis revealed 1-7 fragments per sample. No difference in the bifidobacterial count or in the diversity or stability of bifidobacterial population in general was observed between IBS and control groups. However, a single fragment, identified by 16S rDNA sequencing tentatively as uncultured Bifidobacterium, was more commonly detected in IBS than in control subjects. The identity and role of this fragment in IBS warrants further studies.

AB - Irritable bowel syndrome (IBS) is a chronic intestinal disorder that may involve an altered intestinal microbiota. Although in some studies lower numbers of bifidobacteria have been detected in IBS subjects compared to healthy controls, the composition of bifidobacterial population in IBS subjects is poorly known. The aim of the present study was to investigate the diversity and stability of faecal bifidobacterial population in IBS and control subjects. Faecal samples were obtained from 19 IBS and 16 control subjects at three sampling points (0, 3 and 6 months) and analysed by PCR-DGGE with Bifidobacterium-specific primers Bifl 64-f and Bif662-GC-r. In addition fresh samples from two sampling occasions were cultured on Beerens to determine the bifidobacterial counts. PCR-DGGE analysis revealed 1-7 fragments per sample. No difference in the bifidobacterial count or in the diversity or stability of bifidobacterial population in general was observed between IBS and control groups. However, a single fragment, identified by 16S rDNA sequencing tentatively as uncultured Bifidobacterium, was more commonly detected in IBS than in control subjects. The identity and role of this fragment in IBS warrants further studies.

M3 - Conference abstract in proceedings

SN - 951-38-6289-5

T3 - VTT Symposium

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BT - The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop

PB - VTT Technical Research Centre of Finland

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Mättö J, Saarela M. Composition of bifidobacterial population in irritable bowel syndrome and control subjects. In The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop: Abstracts and posters. Espoo: VTT Technical Research Centre of Finland. 2004. p. 83-83. (VTT Symposium; No. 232).