Composition of bifidobacterial population in irritable bowel syndrome and control subjects

Jaana Mättö, Maria Saarela

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    Irritable bowel syndrome (IBS) is a chronic intestinal disorder that may involve an altered intestinal microbiota. Although in some studies lower numbers of bifidobacteria have been detected in IBS subjects compared to healthy controls, the composition of bifidobacterial population in IBS subjects is poorly known. The aim of the present study was to investigate the diversity and stability of faecal bifidobacterial population in IBS and control subjects. Faecal samples were obtained from 19 IBS and 16 control subjects at three sampling points (0, 3 and 6 months) and analysed by PCR-DGGE with Bifidobacterium-specific primers Bifl 64-f and Bif662-GC-r. In addition fresh samples from two sampling occasions were cultured on Beerens to determine the bifidobacterial counts. PCR-DGGE analysis revealed 1-7 fragments per sample. No difference in the bifidobacterial count or in the diversity or stability of bifidobacterial population in general was observed between IBS and control groups. However, a single fragment, identified by 16S rDNA sequencing tentatively as uncultured Bifidobacterium, was more commonly detected in IBS than in control subjects. The identity and role of this fragment in IBS warrants further studies.
    Original languageEnglish
    Title of host publicationThe Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop
    Subtitle of host publicationAbstracts and posters
    Place of PublicationEspoo
    PublisherVTT Technical Research Centre of Finland
    Pages83-83
    ISBN (Electronic)951-38-6290-9
    ISBN (Print)951-38-6289-5
    Publication statusPublished - 2004
    EventThe Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop - Sitges, Spain
    Duration: 15 Mar 200417 Mar 2004

    Publication series

    SeriesVTT Symposium
    Number232
    ISSN0357-9387

    Workshop

    WorkshopThe Food, GI-tract Functionality and Human Health Cluster
    Country/TerritorySpain
    CitySitges
    Period15/03/0417/03/04

    Fingerprint

    Dive into the research topics of 'Composition of bifidobacterial population in irritable bowel syndrome and control subjects'. Together they form a unique fingerprint.

    Cite this