Abstract
Conformations of the regulatory domain of cardiac troponin C (cNTnC) were studied by means of residual dipolar couplings measured from samples dissolved in dilute liquid crystals. Changes in the main chain HN residual dipolar couplings revealed a conformational change in cNTnC due to the complexation with the second binding region (amino acids 148–163) of cardiac troponin I (cTnI). Formation of the complex is accompanied with a molecular realignment in the liquid crystal. The residual dipolar couplings measured for apo‐cNTnC and the complex with TnI were in agreement with the values computed from the corresponding closed and open solution structures, whereas for the calcium‐loaded conformation the correlation and quality factor were only modest. Ca2+‐cNTnC may be subject to conformational exchange. The data support the model that cardiac troponin C functions as a calcium‐dependent open–closed switch, such as the skeletal troponin C.
| Original language | English |
|---|---|
| Pages (from-to) | 6665 - 6672 |
| Number of pages | 8 |
| Journal | European Journal of Biochemistry |
| Volume | 267 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - 2000 |
| MoE publication type | A1 Journal article-refereed |
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Conformations of the regulatory domain of cardiac troponin C examined by residual dipolar couplings. / Pääkkönen, Kimmo; Sorsa, T.; Drakenberg, Torbjörn; Pollesello, P.; Tilgmann, C.; Permi, P.; Heikkinen, S.; Kilpeläinen, I.; Annila, Arto (Corresponding Author).
In: European Journal of Biochemistry, Vol. 267, No. 22, 2000, p. 6665 - 6672.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Conformations of the regulatory domain of cardiac troponin C examined by residual dipolar couplings
AU - Pääkkönen, Kimmo
AU - Sorsa, T.
AU - Drakenberg, Torbjörn
AU - Pollesello, P.
AU - Tilgmann, C.
AU - Permi, P.
AU - Heikkinen, S.
AU - Kilpeläinen, I.
AU - Annila, Arto
PY - 2000
Y1 - 2000
N2 - Conformations of the regulatory domain of cardiac troponin C (cNTnC) were studied by means of residual dipolar couplings measured from samples dissolved in dilute liquid crystals. Changes in the main chain HN residual dipolar couplings revealed a conformational change in cNTnC due to the complexation with the second binding region (amino acids 148–163) of cardiac troponin I (cTnI). Formation of the complex is accompanied with a molecular realignment in the liquid crystal. The residual dipolar couplings measured for apo‐cNTnC and the complex with TnI were in agreement with the values computed from the corresponding closed and open solution structures, whereas for the calcium‐loaded conformation the correlation and quality factor were only modest. Ca2+‐cNTnC may be subject to conformational exchange. The data support the model that cardiac troponin C functions as a calcium‐dependent open–closed switch, such as the skeletal troponin C.
AB - Conformations of the regulatory domain of cardiac troponin C (cNTnC) were studied by means of residual dipolar couplings measured from samples dissolved in dilute liquid crystals. Changes in the main chain HN residual dipolar couplings revealed a conformational change in cNTnC due to the complexation with the second binding region (amino acids 148–163) of cardiac troponin I (cTnI). Formation of the complex is accompanied with a molecular realignment in the liquid crystal. The residual dipolar couplings measured for apo‐cNTnC and the complex with TnI were in agreement with the values computed from the corresponding closed and open solution structures, whereas for the calcium‐loaded conformation the correlation and quality factor were only modest. Ca2+‐cNTnC may be subject to conformational exchange. The data support the model that cardiac troponin C functions as a calcium‐dependent open–closed switch, such as the skeletal troponin C.
U2 - 10.1046/j.1432-1327.2000.01763.x
DO - 10.1046/j.1432-1327.2000.01763.x
M3 - Article
VL - 267
SP - 6665
EP - 6672
JO - FEBS Journal
JF - FEBS Journal
SN - 1742-464X
IS - 22
ER -