Construction and characterization of cellulolytic yeasts: Dissertation

Merja Penttila

Construction and characterization of cellulolytic yeasts: Dissertation

Research output: Thesis › Dissertation › Collection of Articles

Abstract

The successful construction of cellulolytic yeast strains could lead to improvements in the traditional processes, such as brewing, distilling and wine making, and to more versatile utilization of cellulose for energy production and in novel yeast based applications. Five different cellulolytic yeast strains were constructed in this study. The genes coding for the cellobiohydrolases CBHI and CBHII, and the endoglucanases EGI and EGIII of the filamentous fungus Trichoderma reesei, as well as the beta -glucosidase gene of Aspergillus niger, were expressed in the yeast S. cerevisiae. All recombinant yeast strains were able to hydrolyse natural cellulosic substrates present in yeast culture medium showing different cellulolytic capabilities.

Original language	English
Qualification	Doctor Degree
Awarding Institution	University of Helsinki
Supervisors/Advisors	Knowles, Jonathan K.C., Supervisor, External person
Award date	28 Aug 1987
Publisher	VTT Technical Research Centre of Finland
Print ISBNs	951-38-2928-6
Publication status	Published - 1 May 1987
MoE publication type	G5 Doctoral dissertation (article)

Cite this

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abstract = "The successful construction of cellulolytic yeast strains could lead to improvements in the traditional processes, such as brewing, distilling and wine making, and to more versatile utilization of cellulose for energy production and in novel yeast based applications. Five different cellulolytic yeast strains were constructed in this study. The genes coding for the cellobiohydrolases CBHI and CBHII, and the endoglucanases EGI and EGIII of the filamentous fungus Trichoderma reesei, as well as the beta -glucosidase gene of Aspergillus niger, were expressed in the yeast S. cerevisiae. All recombinant yeast strains were able to hydrolyse natural cellulosic substrates present in yeast culture medium showing different cellulolytic capabilities.",

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N2 - The successful construction of cellulolytic yeast strains could lead to improvements in the traditional processes, such as brewing, distilling and wine making, and to more versatile utilization of cellulose for energy production and in novel yeast based applications. Five different cellulolytic yeast strains were constructed in this study. The genes coding for the cellobiohydrolases CBHI and CBHII, and the endoglucanases EGI and EGIII of the filamentous fungus Trichoderma reesei, as well as the beta -glucosidase gene of Aspergillus niger, were expressed in the yeast S. cerevisiae. All recombinant yeast strains were able to hydrolyse natural cellulosic substrates present in yeast culture medium showing different cellulolytic capabilities.

AB - The successful construction of cellulolytic yeast strains could lead to improvements in the traditional processes, such as brewing, distilling and wine making, and to more versatile utilization of cellulose for energy production and in novel yeast based applications. Five different cellulolytic yeast strains were constructed in this study. The genes coding for the cellobiohydrolases CBHI and CBHII, and the endoglucanases EGI and EGIII of the filamentous fungus Trichoderma reesei, as well as the beta -glucosidase gene of Aspergillus niger, were expressed in the yeast S. cerevisiae. All recombinant yeast strains were able to hydrolyse natural cellulosic substrates present in yeast culture medium showing different cellulolytic capabilities.

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ER -

Construction and characterization of cellulolytic yeasts: Dissertation

Abstract

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