Construction and characterization of cellulolytic yeasts: Dissertation

Research output: ThesisDissertation

2 Citations (Scopus)

Abstract

The successful construction of cellulolytic yeast strains could lead to improvements in the traditional processes, such as brewing, distilling and wine making, and to more versatile utilization of cellulose for energy production and in novel yeast based applications. Five different cellulolytic yeast strains were constructed in this study. The genes coding for the cellobiohydrolases CBHI and CBHII, and the endoglucanases EGI and EGIII of the filamentous fungus Trichoderma reesei, as well as the beta -glucosidase gene of Aspergillus niger, were expressed in the yeast S. cerevisiae. All recombinant yeast strains were able to hydrolyse natural cellulosic substrates present in yeast culture medium showing different cellulolytic capabilities.

Original languageEnglish
QualificationDoctor Degree
Awarding Institution
  • University of Helsinki
Supervisors/Advisors
  • Knowles, Jonathan K.C., Supervisor, External person
Award date28 Aug 1987
Publisher
Print ISBNs951-38-2928-6
Publication statusPublished - 1 May 1987
MoE publication typeG5 Doctoral dissertation (article)

Fingerprint

Yeast
Genes
Brewing
Wine
Aspergillus
Fungi
Cellulose
Substrates

Cite this

Penttila, Merja. / Construction and characterization of cellulolytic yeasts : Dissertation. VTT Technical Research Centre of Finland, 1987. 53 p.
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Construction and characterization of cellulolytic yeasts : Dissertation. / Penttila, Merja.

VTT Technical Research Centre of Finland, 1987. 53 p.

Research output: ThesisDissertation

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T2 - Dissertation

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N2 - The successful construction of cellulolytic yeast strains could lead to improvements in the traditional processes, such as brewing, distilling and wine making, and to more versatile utilization of cellulose for energy production and in novel yeast based applications. Five different cellulolytic yeast strains were constructed in this study. The genes coding for the cellobiohydrolases CBHI and CBHII, and the endoglucanases EGI and EGIII of the filamentous fungus Trichoderma reesei, as well as the beta -glucosidase gene of Aspergillus niger, were expressed in the yeast S. cerevisiae. All recombinant yeast strains were able to hydrolyse natural cellulosic substrates present in yeast culture medium showing different cellulolytic capabilities.

AB - The successful construction of cellulolytic yeast strains could lead to improvements in the traditional processes, such as brewing, distilling and wine making, and to more versatile utilization of cellulose for energy production and in novel yeast based applications. Five different cellulolytic yeast strains were constructed in this study. The genes coding for the cellobiohydrolases CBHI and CBHII, and the endoglucanases EGI and EGIII of the filamentous fungus Trichoderma reesei, as well as the beta -glucosidase gene of Aspergillus niger, were expressed in the yeast S. cerevisiae. All recombinant yeast strains were able to hydrolyse natural cellulosic substrates present in yeast culture medium showing different cellulolytic capabilities.

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Penttila M. Construction and characterization of cellulolytic yeasts: Dissertation. VTT Technical Research Centre of Finland, 1987. 53 p.