Abstract
A flocculent Saccharomyces cerevisiae strain secreting Aspergillus niger β-galactosidase activity was constructed by transforming S. cerevisiae NCYC869-A3 strain with plasmid pVK1.1 harboring the A. niger β-galactosidase gene, lacA, under the control of the ADH1 promoter and terminator. Compared to other recombinant S. cerevisiae strains, this recombinant yeast has higher levels of extracellular β-galactosidase activity. In shake-flask cultures, the β-galactosidase activity detected in the supernatant was 20 times higher than that obtained with previously constructed strains (Domingues et al. 2000a). In bioreactor culture, with cheese-whey permeate as substrate, a yield of 878.0 nkat/gsubstrate was obtained. The recombinant strain is an attractive alternative to other fungal β-galactosidase production systems as the enzyme is produced in a rather pure form. Moreover, the use of flocculating yeast cells allows for enzyme production with high productivity in continuous fermentation systems with facilitated downstream processing.
| Original language | English |
|---|---|
| Pages (from-to) | 645-650 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 58 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 22 Apr 2002 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Fermentation
- Saccharomyces cerevisiae
- Aspergillus niger
- Recombinant strain
- continuous fermentation
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Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger β-galactosidase. / Domingues, L.; Teixeira, J.; Penttilä, M.; Lima, N.
In: Applied Microbiology and Biotechnology, Vol. 58, No. 5, 22.04.2002, p. 645-650.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger β-galactosidase
AU - Domingues, L.
AU - Teixeira, J.
AU - Penttilä, M.
AU - Lima, N.
PY - 2002/4/22
Y1 - 2002/4/22
N2 - A flocculent Saccharomyces cerevisiae strain secreting Aspergillus niger β-galactosidase activity was constructed by transforming S. cerevisiae NCYC869-A3 strain with plasmid pVK1.1 harboring the A. niger β-galactosidase gene, lacA, under the control of the ADH1 promoter and terminator. Compared to other recombinant S. cerevisiae strains, this recombinant yeast has higher levels of extracellular β-galactosidase activity. In shake-flask cultures, the β-galactosidase activity detected in the supernatant was 20 times higher than that obtained with previously constructed strains (Domingues et al. 2000a). In bioreactor culture, with cheese-whey permeate as substrate, a yield of 878.0 nkat/gsubstrate was obtained. The recombinant strain is an attractive alternative to other fungal β-galactosidase production systems as the enzyme is produced in a rather pure form. Moreover, the use of flocculating yeast cells allows for enzyme production with high productivity in continuous fermentation systems with facilitated downstream processing.
AB - A flocculent Saccharomyces cerevisiae strain secreting Aspergillus niger β-galactosidase activity was constructed by transforming S. cerevisiae NCYC869-A3 strain with plasmid pVK1.1 harboring the A. niger β-galactosidase gene, lacA, under the control of the ADH1 promoter and terminator. Compared to other recombinant S. cerevisiae strains, this recombinant yeast has higher levels of extracellular β-galactosidase activity. In shake-flask cultures, the β-galactosidase activity detected in the supernatant was 20 times higher than that obtained with previously constructed strains (Domingues et al. 2000a). In bioreactor culture, with cheese-whey permeate as substrate, a yield of 878.0 nkat/gsubstrate was obtained. The recombinant strain is an attractive alternative to other fungal β-galactosidase production systems as the enzyme is produced in a rather pure form. Moreover, the use of flocculating yeast cells allows for enzyme production with high productivity in continuous fermentation systems with facilitated downstream processing.
KW - Fermentation
KW - Saccharomyces cerevisiae
KW - Aspergillus niger
KW - Recombinant strain
KW - continuous fermentation
UR - http://www.scopus.com/inward/record.url?scp=0036225305&partnerID=8YFLogxK
U2 - 10.1007/s00253-002-0948-1
DO - 10.1007/s00253-002-0948-1
M3 - Article
C2 - 11956748
AN - SCOPUS:0036225305
VL - 58
SP - 645
EP - 650
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -