Construction of brewer's yeasts secreting fungal endo-ß-glucanase

M. E. Penttilä, M. L. Suihko, U. Lehtinen, M. Nikkola, J. K.C. Knowles

Research output: Contribution to journalArticleScientificpeer-review

42 Citations (Scopus)

Abstract

Barley β-glucans present in wort reduce beer filtrability and cause hazes and precipitates in the finished beer. The endo-β-1,4-glucanase enzyme, EGI, found in the filamentous fungus Trichoderma reesei, is capable of efficiently hydrolyzing these β-glucans. The cDNA copy of the eg11 gene, which codes for the EGI enzyme, was coupled to yeast regulatory sequences and transferred to a brewer's yeast using the yeast copper chelatin gene CUP1 as a selection marker in the transformation. The eg11 gene was transferred to the yeast both on a multicopy plasmid and on an integrating plasmid. In both cases, highly glycosylated, active EGI enzyme was secreted into the medium. Barley β-glucans present in wort were efficiently hydrolyzed by the recombinant brewer's yeast.

Original languageEnglish
Pages (from-to)413-420
JournalCurrent Genetics
Volume12
Issue number6
DOIs
Publication statusPublished - 1 Oct 1987
MoE publication typeA1 Journal article-refereed

Keywords

  • Chromosomal integration
  • Endo-β-1,4-glucanase
  • Glucanolytic brewer's yeast
  • Saccharomyces cerevisiae
  • Transformation

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