Construction of brewer's yeasts secreting fungal endo-ß-glucanase

M. E. Penttilä, M. L. Suihko, U. Lehtinen, M. Nikkola, J. K.C. Knowles

Research output: Contribution to journalArticleScientificpeer-review

42 Citations (Scopus)

Abstract

Barley β-glucans present in wort reduce beer filtrability and cause hazes and precipitates in the finished beer. The endo-β-1,4-glucanase enzyme, EGI, found in the filamentous fungus Trichoderma reesei, is capable of efficiently hydrolyzing these β-glucans. The cDNA copy of the eg11 gene, which codes for the EGI enzyme, was coupled to yeast regulatory sequences and transferred to a brewer's yeast using the yeast copper chelatin gene CUP1 as a selection marker in the transformation. The eg11 gene was transferred to the yeast both on a multicopy plasmid and on an integrating plasmid. In both cases, highly glycosylated, active EGI enzyme was secreted into the medium. Barley β-glucans present in wort were efficiently hydrolyzed by the recombinant brewer's yeast.

Original languageEnglish
Pages (from-to)413-420
JournalCurrent Genetics
Volume12
Issue number6
DOIs
Publication statusPublished - 1 Oct 1987
MoE publication typeA1 Journal article-refereed

Fingerprint

Glucans
Saccharomyces cerevisiae
Yeasts
Hordeum
Plasmids
Enzymes
Genes
Trichoderma
Copper
Fungi
Complementary DNA

Keywords

  • Chromosomal integration
  • Endo-β-1,4-glucanase
  • Glucanolytic brewer's yeast
  • Saccharomyces cerevisiae
  • Transformation

Cite this

Penttilä, M. E., Suihko, M. L., Lehtinen, U., Nikkola, M., & Knowles, J. K. C. (1987). Construction of brewer's yeasts secreting fungal endo-ß-glucanase. Current Genetics, 12(6), 413-420. https://doi.org/10.1007/BF00434818
Penttilä, M. E. ; Suihko, M. L. ; Lehtinen, U. ; Nikkola, M. ; Knowles, J. K.C. / Construction of brewer's yeasts secreting fungal endo-ß-glucanase. In: Current Genetics. 1987 ; Vol. 12, No. 6. pp. 413-420.
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Penttilä, ME, Suihko, ML, Lehtinen, U, Nikkola, M & Knowles, JKC 1987, 'Construction of brewer's yeasts secreting fungal endo-ß-glucanase', Current Genetics, vol. 12, no. 6, pp. 413-420. https://doi.org/10.1007/BF00434818

Construction of brewer's yeasts secreting fungal endo-ß-glucanase. / Penttilä, M. E.; Suihko, M. L.; Lehtinen, U.; Nikkola, M.; Knowles, J. K.C.

In: Current Genetics, Vol. 12, No. 6, 01.10.1987, p. 413-420.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Knowles, J. K.C.

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