TY - JOUR
T1 - Construction of flocculent brewer's yeast by chromosomal integration of the yeast flocculation gene FLO1
AU - Watari, Junji
AU - Nomura, Masahiro
AU - Sahara, Hirohisa
AU - Koshino, Shohei
AU - Keränen, Sirkka
N1 - Project code: BEL1019
PY - 1994
Y1 - 1994
N2 - Yeast flocculation gene FLO1, located on chromosome I of Saccharomyces cerevisiae, has been cloned previously16. However, it has recently been found that the gene was an in‐frame deletion derivative of the chromosomal intact FLO1 gene19. When introduced into non‐flocculent industrial strains, including brewer's yeast, the latter gene, FLO1L, containing an open reading frame of 4,611 bp, conferred stronger flocculation than the former gene, FLO1S, containing an open reading frame of 2,586. By chromosomal integration of the ADH1‐controlled FLO1L gene, “gene therapy” of the flocculation behaviour of the parent non‐flocculent brewer's yeast was successfully achieved.
AB - Yeast flocculation gene FLO1, located on chromosome I of Saccharomyces cerevisiae, has been cloned previously16. However, it has recently been found that the gene was an in‐frame deletion derivative of the chromosomal intact FLO1 gene19. When introduced into non‐flocculent industrial strains, including brewer's yeast, the latter gene, FLO1L, containing an open reading frame of 4,611 bp, conferred stronger flocculation than the former gene, FLO1S, containing an open reading frame of 2,586. By chromosomal integration of the ADH1‐controlled FLO1L gene, “gene therapy” of the flocculation behaviour of the parent non‐flocculent brewer's yeast was successfully achieved.
U2 - 10.1002/j.2050-0416.1994.tb00809.x
DO - 10.1002/j.2050-0416.1994.tb00809.x
M3 - Article
SN - 0046-9750
VL - 100
SP - 73
EP - 77
JO - Journal of the Institute of Brewing
JF - Journal of the Institute of Brewing
IS - 2
ER -