Construction of flocculent brewer's yeast by chromosomal integration of the yeast flocculation gene FLO1

Junji Watari, Sirkka Keränen, Masahiro Nomura, Hirohisa Sahara, Shohei Koshino

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

Yeast flocculation gene FLO1, located on chromosome I of Saccharomyces cerevisiae, has been cloned previously16. However, it has recently been found that the gene was an in‐frame deletion derivative of the chromosomal intact FLO1 gene19. When introduced into non‐flocculent industrial strains, including brewer's yeast, the latter gene, FLO1L, containing an open reading frame of 4,611 bp, conferred stronger flocculation than the former gene, FLO1S, containing an open reading frame of 2,586. By chromosomal integration of the ADH1‐controlled FLO1L gene, “gene therapy” of the flocculation behaviour of the parent non‐flocculent brewer's yeast was successfully achieved.
Original languageEnglish
Pages (from-to)73-77
Number of pages5
JournalJournal of the Institute of Brewing
Volume100
Issue number2
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

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