Construction of flocculent brewer's yeast by chromosomal integration of the yeast flocculation gene FLO1

Junji Watari, Sirkka Keränen, Masahiro Nomura, Hirohisa Sahara, Shohei Koshino

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

Yeast flocculation gene FLO1, located on chromosome I of Saccharomyces cerevisiae, has been cloned previously16. However, it has recently been found that the gene was an in‐frame deletion derivative of the chromosomal intact FLO1 gene19. When introduced into non‐flocculent industrial strains, including brewer's yeast, the latter gene, FLO1L, containing an open reading frame of 4,611 bp, conferred stronger flocculation than the former gene, FLO1S, containing an open reading frame of 2,586. By chromosomal integration of the ADH1‐controlled FLO1L gene, “gene therapy” of the flocculation behaviour of the parent non‐flocculent brewer's yeast was successfully achieved.
Original languageEnglish
Pages (from-to)73-77
Number of pages5
JournalJournal of the Institute of Brewing
Volume100
Issue number2
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

Fingerprint

Flocculation
brewers yeast
flocculation
Saccharomyces cerevisiae
Yeasts
yeasts
Genes
genes
Open Reading Frames
open reading frames
gene therapy
Genetic Therapy
Chromosomes
chromosomes

Cite this

Watari, Junji ; Keränen, Sirkka ; Nomura, Masahiro ; Sahara, Hirohisa ; Koshino, Shohei. / Construction of flocculent brewer's yeast by chromosomal integration of the yeast flocculation gene FLO1. In: Journal of the Institute of Brewing. 1994 ; Vol. 100, No. 2. pp. 73-77.
@article{649cd2adb99a4377a2473f21d7d28ecb,
title = "Construction of flocculent brewer's yeast by chromosomal integration of the yeast flocculation gene FLO1",
abstract = "Yeast flocculation gene FLO1, located on chromosome I of Saccharomyces cerevisiae, has been cloned previously16. However, it has recently been found that the gene was an in‐frame deletion derivative of the chromosomal intact FLO1 gene19. When introduced into non‐flocculent industrial strains, including brewer's yeast, the latter gene, FLO1L, containing an open reading frame of 4,611 bp, conferred stronger flocculation than the former gene, FLO1S, containing an open reading frame of 2,586. By chromosomal integration of the ADH1‐controlled FLO1L gene, “gene therapy” of the flocculation behaviour of the parent non‐flocculent brewer's yeast was successfully achieved.",
author = "Junji Watari and Sirkka Ker{\"a}nen and Masahiro Nomura and Hirohisa Sahara and Shohei Koshino",
note = "Project code: BEL1019",
year = "1994",
doi = "10.1002/j.2050-0416.1994.tb00809.x",
language = "English",
volume = "100",
pages = "73--77",
journal = "Journal of the Institute of Brewing",
issn = "0046-9750",
publisher = "Wiley",
number = "2",

}

Construction of flocculent brewer's yeast by chromosomal integration of the yeast flocculation gene FLO1. / Watari, Junji; Keränen, Sirkka; Nomura, Masahiro; Sahara, Hirohisa; Koshino, Shohei.

In: Journal of the Institute of Brewing, Vol. 100, No. 2, 1994, p. 73-77.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Construction of flocculent brewer's yeast by chromosomal integration of the yeast flocculation gene FLO1

AU - Watari, Junji

AU - Keränen, Sirkka

AU - Nomura, Masahiro

AU - Sahara, Hirohisa

AU - Koshino, Shohei

N1 - Project code: BEL1019

PY - 1994

Y1 - 1994

N2 - Yeast flocculation gene FLO1, located on chromosome I of Saccharomyces cerevisiae, has been cloned previously16. However, it has recently been found that the gene was an in‐frame deletion derivative of the chromosomal intact FLO1 gene19. When introduced into non‐flocculent industrial strains, including brewer's yeast, the latter gene, FLO1L, containing an open reading frame of 4,611 bp, conferred stronger flocculation than the former gene, FLO1S, containing an open reading frame of 2,586. By chromosomal integration of the ADH1‐controlled FLO1L gene, “gene therapy” of the flocculation behaviour of the parent non‐flocculent brewer's yeast was successfully achieved.

AB - Yeast flocculation gene FLO1, located on chromosome I of Saccharomyces cerevisiae, has been cloned previously16. However, it has recently been found that the gene was an in‐frame deletion derivative of the chromosomal intact FLO1 gene19. When introduced into non‐flocculent industrial strains, including brewer's yeast, the latter gene, FLO1L, containing an open reading frame of 4,611 bp, conferred stronger flocculation than the former gene, FLO1S, containing an open reading frame of 2,586. By chromosomal integration of the ADH1‐controlled FLO1L gene, “gene therapy” of the flocculation behaviour of the parent non‐flocculent brewer's yeast was successfully achieved.

U2 - 10.1002/j.2050-0416.1994.tb00809.x

DO - 10.1002/j.2050-0416.1994.tb00809.x

M3 - Article

VL - 100

SP - 73

EP - 77

JO - Journal of the Institute of Brewing

JF - Journal of the Institute of Brewing

SN - 0046-9750

IS - 2

ER -