Abstract
| Original language | English |
|---|---|
| Title of host publication | Recombinant Proteins from Plants |
| Subtitle of host publication | Methods and Protocols |
| Editors | Jacqueline MacDonald, Igor Kolotilin, Rima Menassa |
| Publisher | Springer |
| Pages | 189-197 |
| Edition | Second |
| ISBN (Electronic) | 978-1-4939-3289-4 |
| ISBN (Print) | 978-1-4939-3288-7 |
| DOIs | |
| Publication status | Published - 2016 |
| MoE publication type | D2 Article in professional manuals or guides or professional information systems or text book material |
Publication series
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 1385 |
| ISSN | 1064-3745 |
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Keywords
- continuous aqueous two-phase system
- ATPS
- hydrophobin
- HFBI
- protein purification
- protein fusion
Cite this
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Continuous flow separation of hydrophobin fusion proteins from plant cell culture extract. / Reuter, Lauri; Conley, Andrew; Joensuu, Jussi (Corresponding author).
Recombinant Proteins from Plants: Methods and Protocols. ed. / Jacqueline MacDonald; Igor Kolotilin; Rima Menassa. Second. ed. Springer, 2016. p. 189-197 (Methods in Molecular Biology, Vol. 1385).Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Professional
TY - CHAP
T1 - Continuous flow separation of hydrophobin fusion proteins from plant cell culture extract
AU - Reuter, Lauri
AU - Conley, Andrew
AU - Joensuu, Jussi
PY - 2016
Y1 - 2016
N2 - Fusion to fungal hydrophobins has proven to be a useful tool to enhance accumulation and recovery of recombinant proteins in plants. Aqueous two-phase separation (ATPS) is an attractive system to capture hydrophobin fusion proteins from plant extracts. The process can simultaneously purify and concentrate target protein with minimal background. ATPS avoids the use of chromatographic column steps, can be carried out in a short time frame, and is amenable to industrial-scale protein purification. A drawback of performing ATPS in large volumes is the lengthy time required for phase separation; however, this can be avoided by incorporating continuous systems, which are often preferred by the processing industry. This method chapter illustrates the capture of GFP-HFBI hydrophobin fusion protein from BY-2 plant cell suspension extract using a semi-continuous ATPS method.
AB - Fusion to fungal hydrophobins has proven to be a useful tool to enhance accumulation and recovery of recombinant proteins in plants. Aqueous two-phase separation (ATPS) is an attractive system to capture hydrophobin fusion proteins from plant extracts. The process can simultaneously purify and concentrate target protein with minimal background. ATPS avoids the use of chromatographic column steps, can be carried out in a short time frame, and is amenable to industrial-scale protein purification. A drawback of performing ATPS in large volumes is the lengthy time required for phase separation; however, this can be avoided by incorporating continuous systems, which are often preferred by the processing industry. This method chapter illustrates the capture of GFP-HFBI hydrophobin fusion protein from BY-2 plant cell suspension extract using a semi-continuous ATPS method.
KW - continuous aqueous two-phase system
KW - ATPS
KW - hydrophobin
KW - HFBI
KW - protein purification
KW - protein fusion
U2 - 10.1007/978-1-4939-3289-4_14
DO - 10.1007/978-1-4939-3289-4_14
M3 - Chapter or book article
SN - 978-1-4939-3288-7
T3 - Methods in Molecular Biology
SP - 189
EP - 197
BT - Recombinant Proteins from Plants
A2 - MacDonald, Jacqueline
A2 - Kolotilin, Igor
A2 - Menassa, Rima
PB - Springer
ER -