Control and regulation of metabolic fluxes in microbes by substrates and enzymes

K. van Dam, N. Jansen, P. Postma, P. Richard, G. Ruijter, M. Rutgers, H. P. Smits, B. Teusink, J. van der Vlag, M. Walsh, H. V. Westerhoff

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

The control of enzymes and substrates on the flux through microbial metabolic pathways can be quantified in terms of flux control coefficients. In pathways involving group transfer, the summation theorem for flux control by the enzymes has to be modified: the sum of control by all enzymes is between 1 and 2. The phosphoenolpyruvate:glucose phosphotransferase system is such a pathway. Experimental determination of the control by the enzymes in this pathway is under way. The control of the enzymes on the glycolytic flux in yeast is low, with the possible exception of the uptake step. In Klebsiella pneumoniae potassium and ammonium ions can simultaneously be 'limiting', (i.e. have significant control on growth) at pH 6, but not at pH 8. This may be due to the fact that at pH 8 the high-affinity potassium uptake system is absent.

Original languageEnglish
Pages (from-to)315-321
Number of pages7
JournalAntonie van Leeuwenhoek
Volume63
Issue number3-4
DOIs
Publication statusPublished - 1 Sep 1993
MoE publication typeNot Eligible

Fingerprint

Enzymes
Potassium
Klebsiella pneumoniae
Metabolic Networks and Pathways
Ammonium Compounds
Yeasts
Ions
Growth
phosphoenolpyruvate-glucose phosphotransferase

Keywords

  • (microbial) metabolism
  • control
  • enzyme
  • growth
  • substrate

Cite this

van Dam, K., Jansen, N., Postma, P., Richard, P., Ruijter, G., Rutgers, M., ... Westerhoff, H. V. (1993). Control and regulation of metabolic fluxes in microbes by substrates and enzymes. Antonie van Leeuwenhoek, 63(3-4), 315-321. https://doi.org/10.1007/BF00871226
van Dam, K. ; Jansen, N. ; Postma, P. ; Richard, P. ; Ruijter, G. ; Rutgers, M. ; Smits, H. P. ; Teusink, B. ; van der Vlag, J. ; Walsh, M. ; Westerhoff, H. V. / Control and regulation of metabolic fluxes in microbes by substrates and enzymes. In: Antonie van Leeuwenhoek. 1993 ; Vol. 63, No. 3-4. pp. 315-321.
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van Dam, K, Jansen, N, Postma, P, Richard, P, Ruijter, G, Rutgers, M, Smits, HP, Teusink, B, van der Vlag, J, Walsh, M & Westerhoff, HV 1993, 'Control and regulation of metabolic fluxes in microbes by substrates and enzymes', Antonie van Leeuwenhoek, vol. 63, no. 3-4, pp. 315-321. https://doi.org/10.1007/BF00871226

Control and regulation of metabolic fluxes in microbes by substrates and enzymes. / van Dam, K.; Jansen, N.; Postma, P.; Richard, P.; Ruijter, G.; Rutgers, M.; Smits, H. P.; Teusink, B.; van der Vlag, J.; Walsh, M.; Westerhoff, H. V.

In: Antonie van Leeuwenhoek, Vol. 63, No. 3-4, 01.09.1993, p. 315-321.

Research output: Contribution to journalArticleScientificpeer-review

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