Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods

Hanna-Leena Alakomi, Maria Saarela, A. A. Gorbushina, W. E. Krumbein, C. McCullagh, P. Robertson, K. Rodenacker

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

4 Citations (Scopus)

Abstract

The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides. © 2006 Taylor & Francis Group. (13 refs.)
Original languageEnglish
Title of host publicationHeritage, Weathering and Conservation
Subtitle of host publicationProceedings of the International Conference on Heritage, Weathering and Conservation, HWC-2006. Madrid, Spain, 21 - 24 June 2006
EditorsR. Fort, M. Alvarez de Buergo, M. Gomez-Heras, C. Vazquez-Calvo
PublisherTaylor & Francis
Pages713-717
Volume2
ISBN (Print)978-0-415-41272-8, 978-0-415-41273-5
Publication statusPublished - 2006
MoE publication typeA4 Article in a conference publication
EventInternational Conference on Heritage, Weathering and Conservation, HWC-2006 - Madrid, Spain
Duration: 21 Jun 200624 Jun 2006

Conference

ConferenceInternational Conference on Heritage, Weathering and Conservation, HWC-2006
CountrySpain
CityMadrid
Period21/06/0624/06/06

Fingerprint

biofilm
inhibitor
microbial community
biodegradation
chemical
evaluation method
monument
image analysis
microscopy
pesticide
alga
fungus
bacterium
atmosphere
mineral
rock

Cite this

Alakomi, H-L., Saarela, M., Gorbushina, A. A., Krumbein, W. E., McCullagh, C., Robertson, P., & Rodenacker, K. (2006). Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods. In R. Fort, M. Alvarez de Buergo, M. Gomez-Heras, & C. Vazquez-Calvo (Eds.), Heritage, Weathering and Conservation: Proceedings of the International Conference on Heritage, Weathering and Conservation, HWC-2006. Madrid, Spain, 21 - 24 June 2006 (Vol. 2, pp. 713-717). Taylor & Francis.
Alakomi, Hanna-Leena ; Saarela, Maria ; Gorbushina, A. A. ; Krumbein, W. E. ; McCullagh, C. ; Robertson, P. ; Rodenacker, K. / Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods. Heritage, Weathering and Conservation: Proceedings of the International Conference on Heritage, Weathering and Conservation, HWC-2006. Madrid, Spain, 21 - 24 June 2006. editor / R. Fort ; M. Alvarez de Buergo ; M. Gomez-Heras ; C. Vazquez-Calvo. Vol. 2 Taylor & Francis, 2006. pp. 713-717
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title = "Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods",
abstract = "The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as {"}subaerial biofilms{"}. The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides. {\circledC} 2006 Taylor & Francis Group. (13 refs.)",
author = "Hanna-Leena Alakomi and Maria Saarela and Gorbushina, {A. A.} and Krumbein, {W. E.} and C. McCullagh and P. Robertson and K. Rodenacker",
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Alakomi, H-L, Saarela, M, Gorbushina, AA, Krumbein, WE, McCullagh, C, Robertson, P & Rodenacker, K 2006, Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods. in R Fort, M Alvarez de Buergo, M Gomez-Heras & C Vazquez-Calvo (eds), Heritage, Weathering and Conservation: Proceedings of the International Conference on Heritage, Weathering and Conservation, HWC-2006. Madrid, Spain, 21 - 24 June 2006. vol. 2, Taylor & Francis, pp. 713-717, International Conference on Heritage, Weathering and Conservation, HWC-2006, Madrid, Spain, 21/06/06.

Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods. / Alakomi, Hanna-Leena; Saarela, Maria; Gorbushina, A. A.; Krumbein, W. E.; McCullagh, C.; Robertson, P.; Rodenacker, K.

Heritage, Weathering and Conservation: Proceedings of the International Conference on Heritage, Weathering and Conservation, HWC-2006. Madrid, Spain, 21 - 24 June 2006. ed. / R. Fort; M. Alvarez de Buergo; M. Gomez-Heras; C. Vazquez-Calvo. Vol. 2 Taylor & Francis, 2006. p. 713-717.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

TY - GEN

T1 - Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods

AU - Alakomi, Hanna-Leena

AU - Saarela, Maria

AU - Gorbushina, A. A.

AU - Krumbein, W. E.

AU - McCullagh, C.

AU - Robertson, P.

AU - Rodenacker, K.

PY - 2006

Y1 - 2006

N2 - The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides. © 2006 Taylor & Francis Group. (13 refs.)

AB - The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides. © 2006 Taylor & Francis Group. (13 refs.)

M3 - Conference article in proceedings

SN - 978-0-415-41272-8

SN - 978-0-415-41273-5

VL - 2

SP - 713

EP - 717

BT - Heritage, Weathering and Conservation

A2 - Fort, R.

A2 - Alvarez de Buergo, M.

A2 - Gomez-Heras, M.

A2 - Vazquez-Calvo, C.

PB - Taylor & Francis

ER -

Alakomi H-L, Saarela M, Gorbushina AA, Krumbein WE, McCullagh C, Robertson P et al. Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods. In Fort R, Alvarez de Buergo M, Gomez-Heras M, Vazquez-Calvo C, editors, Heritage, Weathering and Conservation: Proceedings of the International Conference on Heritage, Weathering and Conservation, HWC-2006. Madrid, Spain, 21 - 24 June 2006. Vol. 2. Taylor & Francis. 2006. p. 713-717