Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods

Hanna-Leena Alakomi, Maria Saarela, A. A. Gorbushina, W. E. Krumbein, C. McCullagh, P. Robertson, K. Rodenacker

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

4 Citations (Scopus)

Abstract

The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides. © 2006 Taylor & Francis Group. (13 refs.)
Original languageEnglish
Title of host publicationHeritage, Weathering and Conservation
Subtitle of host publicationProceedings of the International Conference on Heritage, Weathering and Conservation, HWC-2006. Madrid, Spain, 21 - 24 June 2006
EditorsR. Fort, M. Alvarez de Buergo, M. Gomez-Heras, C. Vazquez-Calvo
PublisherTaylor & Francis
Pages713-717
Volume2
ISBN (Print)978-0-415-41272-8, 978-0-415-41273-5
Publication statusPublished - 2006
MoE publication typeA4 Article in a conference publication
EventInternational Conference on Heritage, Weathering and Conservation, HWC-2006 - Madrid, Spain
Duration: 21 Jun 200624 Jun 2006

Conference

ConferenceInternational Conference on Heritage, Weathering and Conservation, HWC-2006
CountrySpain
CityMadrid
Period21/06/0624/06/06

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    Alakomi, H-L., Saarela, M., Gorbushina, A. A., Krumbein, W. E., McCullagh, C., Robertson, P., & Rodenacker, K. (2006). Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: In vitro evaluation methods. In R. Fort, M. Alvarez de Buergo, M. Gomez-Heras, & C. Vazquez-Calvo (Eds.), Heritage, Weathering and Conservation: Proceedings of the International Conference on Heritage, Weathering and Conservation, HWC-2006. Madrid, Spain, 21 - 24 June 2006 (Vol. 2, pp. 713-717). Taylor & Francis.