Control of protein affinity of bioactive nanocellulose and passivation using engineered block and random copolymers

Maija Vuoriluoto, Hannes Orelma, Baolei Zhu, Leena Sisko Johansson, Orlando J. Rojas

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.
Original languageEnglish
Pages (from-to)5668-5678
JournalACS Applied Materials & Interfaces
Volume8
Issue number8
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

oxidized cellulose
Passivation
Cellulose
Copolymers
Immunoglobulin G
Proteins
Methyl Ethers
Methacrylates
Staphylococcal Protein A
Antigens
Fouling
Antibodies
Polyethylene glycols
Block copolymers
Ethers
Polymers
TEMPO

Keywords

  • antifouling
  • biosurfaces
  • cellulose nanofibrils
  • human IgG
  • nonspecific adsorption
  • PDMAEMA
  • POEGMA
  • TEMPO-oxidation

Cite this

Vuoriluoto, Maija ; Orelma, Hannes ; Zhu, Baolei ; Johansson, Leena Sisko ; Rojas, Orlando J. / Control of protein affinity of bioactive nanocellulose and passivation using engineered block and random copolymers. In: ACS Applied Materials & Interfaces. 2016 ; Vol. 8, No. 8. pp. 5668-5678.
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abstract = "We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.",
keywords = "antifouling, biosurfaces, cellulose nanofibrils, human IgG, nonspecific adsorption, PDMAEMA, POEGMA, TEMPO-oxidation",
author = "Maija Vuoriluoto and Hannes Orelma and Baolei Zhu and Johansson, {Leena Sisko} and Rojas, {Orlando J.}",
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Control of protein affinity of bioactive nanocellulose and passivation using engineered block and random copolymers. / Vuoriluoto, Maija; Orelma, Hannes; Zhu, Baolei; Johansson, Leena Sisko; Rojas, Orlando J.

In: ACS Applied Materials & Interfaces, Vol. 8, No. 8, 2016, p. 5668-5678.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Control of protein affinity of bioactive nanocellulose and passivation using engineered block and random copolymers

AU - Vuoriluoto, Maija

AU - Orelma, Hannes

AU - Zhu, Baolei

AU - Johansson, Leena Sisko

AU - Rojas, Orlando J.

PY - 2016

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N2 - We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.

AB - We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.

KW - antifouling

KW - biosurfaces

KW - cellulose nanofibrils

KW - human IgG

KW - nonspecific adsorption

KW - PDMAEMA

KW - POEGMA

KW - TEMPO-oxidation

U2 - 10.1021/acsami.5b11737

DO - 10.1021/acsami.5b11737

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EP - 5678

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

SN - 1944-8244

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