Control of protein affinity of bioactive nanocellulose and passivation using engineered block and random copolymers

Maija Vuoriluoto, Hannes Orelma, Baolei Zhu, Leena Sisko Johansson, Orlando J. Rojas

    Research output: Contribution to journalArticleScientificpeer-review

    14 Citations (Scopus)

    Abstract

    We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.
    Original languageEnglish
    Pages (from-to)5668-5678
    JournalACS Applied Materials & Interfaces
    Volume8
    Issue number8
    DOIs
    Publication statusPublished - 2016
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    oxidized cellulose
    Passivation
    Cellulose
    Copolymers
    Immunoglobulin G
    Proteins
    Methyl Ethers
    Methacrylates
    Staphylococcal Protein A
    Antigens
    Fouling
    Antibodies
    Polyethylene glycols
    Block copolymers
    Ethers
    Polymers
    TEMPO

    Keywords

    • antifouling
    • biosurfaces
    • cellulose nanofibrils
    • human IgG
    • nonspecific adsorption
    • PDMAEMA
    • POEGMA
    • TEMPO-oxidation

    Cite this

    Vuoriluoto, Maija ; Orelma, Hannes ; Zhu, Baolei ; Johansson, Leena Sisko ; Rojas, Orlando J. / Control of protein affinity of bioactive nanocellulose and passivation using engineered block and random copolymers. In: ACS Applied Materials & Interfaces. 2016 ; Vol. 8, No. 8. pp. 5668-5678.
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    abstract = "We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.",
    keywords = "antifouling, biosurfaces, cellulose nanofibrils, human IgG, nonspecific adsorption, PDMAEMA, POEGMA, TEMPO-oxidation",
    author = "Maija Vuoriluoto and Hannes Orelma and Baolei Zhu and Johansson, {Leena Sisko} and Rojas, {Orlando J.}",
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    Control of protein affinity of bioactive nanocellulose and passivation using engineered block and random copolymers. / Vuoriluoto, Maija; Orelma, Hannes; Zhu, Baolei; Johansson, Leena Sisko; Rojas, Orlando J.

    In: ACS Applied Materials & Interfaces, Vol. 8, No. 8, 2016, p. 5668-5678.

    Research output: Contribution to journalArticleScientificpeer-review

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    T1 - Control of protein affinity of bioactive nanocellulose and passivation using engineered block and random copolymers

    AU - Vuoriluoto, Maija

    AU - Orelma, Hannes

    AU - Zhu, Baolei

    AU - Johansson, Leena Sisko

    AU - Rojas, Orlando J.

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    N2 - We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.

    AB - We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.

    KW - antifouling

    KW - biosurfaces

    KW - cellulose nanofibrils

    KW - human IgG

    KW - nonspecific adsorption

    KW - PDMAEMA

    KW - POEGMA

    KW - TEMPO-oxidation

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