Abstract
Citrus pulp is a waste product of orange juice processing. Pectin can be extracted from this pulp and used in the food industry. However, citrus pulp is produced in such large amounts that the pectin it contains is far in excess of the current world pectin market. An alternative strategy would be to hydrolyze this excess pectin to liberate D-galacturonic acid (D-galA), which can then be converted into several platform chemicals. We report, for the first time, the hydrolysis of pectin by the direct addition of “pectinolytic fermented solids”. These solids were produced by solid-state fermentation of a 30:70 mixture, by dry mass, of ground sugarcane bagasse and orange peels, using a strain of Aspergillus oryzae isolated from rotting passion fruit peels. With the addition of this lyophilized fermented solid to a 10% w v −1 pectin solution, we obtained 247 mmol L −1 of D-galA in the hydrolysate, this being the highest D-galA concentration yet reported. Since the direct addition of fermented solid to the reaction mixture avoids the need for extraction and recovery steps, our process has potential to provide low cost of pectinases for use in citrus-waste biorefineries.
| Original language | English |
|---|---|
| Pages (from-to) | 214-219 |
| Number of pages | 6 |
| Journal | Biocatalysis and Agricultural Biotechnology |
| Volume | 11 |
| DOIs | |
| Publication status | Published - 2017 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- solid-state fermentation
- D-galacturonic acid
- pectinolytic enzymes
- orange bagasse
- Aspergillus oryzae
- citrus-waste biorefineries
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Conversion of citric pectin into D-galacturonic acid with high substrate loading using a fermented solid with pectinolytic activity. / Leh, Daniele Stock; Biz, Alessandra; de Paula, D.H.F.; Richard, Peter; Gonçalves, Alan Guilherme; Noseda, Miguel Daniel; Mitchell, David Alexander; Kriegera, Nadia.
In: Biocatalysis and Agricultural Biotechnology, Vol. 11, 2017, p. 214-219.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Conversion of citric pectin into D-galacturonic acid with high substrate loading using a fermented solid with pectinolytic activity
AU - Leh, Daniele Stock
AU - Biz, Alessandra
AU - de Paula, D.H.F.
AU - Richard, Peter
AU - Gonçalves, Alan Guilherme
AU - Noseda, Miguel Daniel
AU - Mitchell, David Alexander
AU - Kriegera, Nadia
PY - 2017
Y1 - 2017
N2 - Citrus pulp is a waste product of orange juice processing. Pectin can be extracted from this pulp and used in the food industry. However, citrus pulp is produced in such large amounts that the pectin it contains is far in excess of the current world pectin market. An alternative strategy would be to hydrolyze this excess pectin to liberate D-galacturonic acid (D-galA), which can then be converted into several platform chemicals. We report, for the first time, the hydrolysis of pectin by the direct addition of “pectinolytic fermented solids”. These solids were produced by solid-state fermentation of a 30:70 mixture, by dry mass, of ground sugarcane bagasse and orange peels, using a strain of Aspergillus oryzae isolated from rotting passion fruit peels. With the addition of this lyophilized fermented solid to a 10% w v −1 pectin solution, we obtained 247 mmol L −1 of D-galA in the hydrolysate, this being the highest D-galA concentration yet reported. Since the direct addition of fermented solid to the reaction mixture avoids the need for extraction and recovery steps, our process has potential to provide low cost of pectinases for use in citrus-waste biorefineries.
AB - Citrus pulp is a waste product of orange juice processing. Pectin can be extracted from this pulp and used in the food industry. However, citrus pulp is produced in such large amounts that the pectin it contains is far in excess of the current world pectin market. An alternative strategy would be to hydrolyze this excess pectin to liberate D-galacturonic acid (D-galA), which can then be converted into several platform chemicals. We report, for the first time, the hydrolysis of pectin by the direct addition of “pectinolytic fermented solids”. These solids were produced by solid-state fermentation of a 30:70 mixture, by dry mass, of ground sugarcane bagasse and orange peels, using a strain of Aspergillus oryzae isolated from rotting passion fruit peels. With the addition of this lyophilized fermented solid to a 10% w v −1 pectin solution, we obtained 247 mmol L −1 of D-galA in the hydrolysate, this being the highest D-galA concentration yet reported. Since the direct addition of fermented solid to the reaction mixture avoids the need for extraction and recovery steps, our process has potential to provide low cost of pectinases for use in citrus-waste biorefineries.
KW - solid-state fermentation
KW - D-galacturonic acid
KW - pectinolytic enzymes
KW - orange bagasse
KW - Aspergillus oryzae
KW - citrus-waste biorefineries
UR - http://www.scopus.com/inward/record.url?scp=85025117401&partnerID=8YFLogxK
U2 - 10.1016/j.bcab.2017.07.003
DO - 10.1016/j.bcab.2017.07.003
M3 - Article
VL - 11
SP - 214
EP - 219
JO - Biocatalysis and Agricultural Biotechnology
JF - Biocatalysis and Agricultural Biotechnology
SN - 1878-8181
ER -