Converting CO2 to biofuels with photosynthetic microalgae immobilized in cellulose nanofibrils

Ville Rissanen, Mikael Jämsä, Sergey Kosourov, J A Ketoja, Yagut Allahverdiyeva, Tekla Tammelin

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

We created versatile templates for biofuel prodn. by using TEMPO-oxidized cellulose nanofibrils (TEMPO CNF) to immobilize photosynthetically active microalgae. In contrast to conventional suspension cultures, entrapping microalgae within a fibrillar matrix greatly enhance cell fitness and viability. The innate hydrophilic and hygroscopic nature and high surface area of TEMPO CNF create a system with favorable water transition properties, while the nanoscale fibrillar network provides mech. stability as well as control over the d./porosity structure. Furthermore, its transparency in the photosynthetically active radiation (PAR) region ensures optimal light utilization and enhanced photosynthetic productivity. To demonstrate this, we entrapped H2-producing microalgae in TEMPO CNF via three immobilization approaches: a pure TEMPO CNF hydrogel, a Ca2+ -stabilized TEMPO CNF hydrogel (Fig. 1A) and a chem. cross-linked TEMPO CNF film (Fig. 1B). All approaches showcased excellent biol. compatibility, as well as good H2 prodn. capacity with similar or higher values compared to the conventionally used alginate-based matrixes. The surface interactions between microalgae and TEMPO CNF were also revealed using Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D). We found that the anionic surface charges of both microalgae cells and TEMPO CNF prevent surface attachment, leading to immobilization via passive entrapment. Conversely, microalgae cells clearly attach to cationized TEMPO CNF surface. Thus, a simple surface modification can further tailor the matrix towards direct cell attachment. These findings will facilitate the development of new approaches for targeted biofuels and chems. prodn. in bio-industry.
Original languageEnglish
Title of host publicationAbstracts of Papers, 257th ACS National Meeting & Exposition, Orlando, FL, United States, Mar. 31-Apr. 4, 2019
PublisherAmerican Chemical Society ACS
Number of pages1
Publication statusPublished - 2019
MoE publication typeNot Eligible
Event257th ACS National Meeting & Exposition - Orlando, United States
Duration: 31 Mar 20194 Apr 2019

Publication series

SeriesAbstracts of Papers: The American Chemical Society
ISSN0065-7727

Conference

Conference257th ACS National Meeting & Exposition
CountryUnited States
CityOrlando
Period31/03/194/04/19

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    Rissanen, V., Jämsä, M., Kosourov, S., Ketoja, J. A., Allahverdiyeva, Y., & Tammelin, T. (2019). Converting CO2 to biofuels with photosynthetic microalgae immobilized in cellulose nanofibrils. In Abstracts of Papers, 257th ACS National Meeting & Exposition, Orlando, FL, United States, Mar. 31-Apr. 4, 2019 [CELL-0314] American Chemical Society ACS. Abstracts of Papers: The American Chemical Society