Abstract
Enzymes remain a significant operational cost in biochemical biorefining processes. Inexpensive streams of sugar are available at many potential biorefining sites, and these could be an optimal raw material for the on-site production of cellulase enzymes. However, simple sugars do not support cellulase production using conventional cellulase-producing strains, necessitating either process or strain improvements. Here we describe improved glucose-based enzyme production processes enabling industrially relevant cellulase production kinetics. Extracellular protein titers of up to 53 g/L could be reached in under 93 hours, representing an up to 260% improvement in productivity over previously reported results. Glucose-produced enzyme is also shown to perform well on pre-treated corn stover. Using the NREL biorefinery model, enzyme production costs are estimated to be $2,8/kg protein or 6/L produced ethanol. Additionally, we briefly discuss how organic nitrogen could be cycled in enzyme production, and how the described processes could fit into existing and emerging biorefining operations.
Original language | English |
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Title of host publication | NWBC 2018 |
Subtitle of host publication | Proceedings of the 8th Nordic Wood Biorefinery Conference |
Editors | Eemeli Hytonen, Jessica Vepsalainen |
Publisher | VTT Technical Research Centre of Finland |
Pages | 185-192 |
Number of pages | 8 |
ISBN (Electronic) | 978-951388671-4 |
ISBN (Print) | 978-951-38-8672-1 |
Publication status | Published - 2018 |
MoE publication type | A4 Article in a conference publication |
Event | 8th Nordic Wood Biorefinery Conference, NWBC 2018 - Scandic Marina Congress Center, Helsinki, Finland Duration: 23 Oct 2018 → 25 Oct 2018 http://bioeconomy.vtt.fi/NWBC2018 (Conference website) |
Publication series
Series | VTT Technology |
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Number | 340 |
ISSN | 2242-1211 |
Conference
Conference | 8th Nordic Wood Biorefinery Conference, NWBC 2018 |
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Country | Finland |
City | Helsinki |
Period | 23/10/18 → 25/10/18 |
Internet address |
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