Coupled size-exclusion chromatography-anion-exchange chromatography in the analysis of poly- and oligosaccharides

Tapani Suortti

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7 Citations (Scopus)

Abstract

The use of anion-exchange chromatography (AEX) in the analysis of oligosaccharides is hampered by the fact that more complicated samples result in a jungle of peaks, which are hard to interpret. In two-dimensional chromatography with a combination of size-exclusion chromatography and AEX, the interpretation is facilitated by the use of hydrodynamic volume data along with AEX data. Therefore, the highly branched and linear gluco-oligosaccharides may be easily distinguished, as well as linear gluco-oligosaccharides with different bonds. Two-dimensional chromatography also provides a simple and reliable method to avoid interferences from buffer components or from major peaks or even from overwhelming concentrations of larger polysaccharides.

Original languageEnglish
Pages (from-to)331 - 335
Number of pages5
JournalJournal of Chromatography A
Volume763
Issue number1-2
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Size exclusion chromatography
Chromatography
Oligosaccharides
Gel Chromatography
Anions
Polysaccharides
Hydrodynamics
Buffers

Cite this

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abstract = "The use of anion-exchange chromatography (AEX) in the analysis of oligosaccharides is hampered by the fact that more complicated samples result in a jungle of peaks, which are hard to interpret. In two-dimensional chromatography with a combination of size-exclusion chromatography and AEX, the interpretation is facilitated by the use of hydrodynamic volume data along with AEX data. Therefore, the highly branched and linear gluco-oligosaccharides may be easily distinguished, as well as linear gluco-oligosaccharides with different bonds. Two-dimensional chromatography also provides a simple and reliable method to avoid interferences from buffer components or from major peaks or even from overwhelming concentrations of larger polysaccharides.",
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Coupled size-exclusion chromatography-anion-exchange chromatography in the analysis of poly- and oligosaccharides. / Suortti, Tapani.

In: Journal of Chromatography A, Vol. 763, No. 1-2, 1997, p. 331 - 335.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Coupled size-exclusion chromatography-anion-exchange chromatography in the analysis of poly- and oligosaccharides

AU - Suortti, Tapani

PY - 1997

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N2 - The use of anion-exchange chromatography (AEX) in the analysis of oligosaccharides is hampered by the fact that more complicated samples result in a jungle of peaks, which are hard to interpret. In two-dimensional chromatography with a combination of size-exclusion chromatography and AEX, the interpretation is facilitated by the use of hydrodynamic volume data along with AEX data. Therefore, the highly branched and linear gluco-oligosaccharides may be easily distinguished, as well as linear gluco-oligosaccharides with different bonds. Two-dimensional chromatography also provides a simple and reliable method to avoid interferences from buffer components or from major peaks or even from overwhelming concentrations of larger polysaccharides.

AB - The use of anion-exchange chromatography (AEX) in the analysis of oligosaccharides is hampered by the fact that more complicated samples result in a jungle of peaks, which are hard to interpret. In two-dimensional chromatography with a combination of size-exclusion chromatography and AEX, the interpretation is facilitated by the use of hydrodynamic volume data along with AEX data. Therefore, the highly branched and linear gluco-oligosaccharides may be easily distinguished, as well as linear gluco-oligosaccharides with different bonds. Two-dimensional chromatography also provides a simple and reliable method to avoid interferences from buffer components or from major peaks or even from overwhelming concentrations of larger polysaccharides.

U2 - 10.1016/S0021-9673(96)00996-X

DO - 10.1016/S0021-9673(96)00996-X

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VL - 763

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EP - 335

JO - Journal of Chromatography A

JF - Journal of Chromatography A

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