Abstract
The use of anion-exchange chromatography (AEX) in the analysis of oligosaccharides is hampered by the fact that more complicated samples result in a jungle of peaks, which are hard to interpret. In two-dimensional chromatography with a combination of size-exclusion chromatography and AEX, the interpretation is facilitated by the use of hydrodynamic volume data along with AEX data. Therefore, the highly branched and linear gluco-oligosaccharides may be easily distinguished, as well as linear gluco-oligosaccharides with different bonds. Two-dimensional chromatography also provides a simple and reliable method to avoid interferences from buffer components or from major peaks or even from overwhelming concentrations of larger polysaccharides.
| Original language | English |
|---|---|
| Pages (from-to) | 331-335 |
| Journal | Journal of Chromatography A |
| Volume | 763 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1997 |
| MoE publication type | A1 Journal article-refereed |