Abstract
Cellobiohydrolases belonging to glycoside hydrolase
family 6 (CBH II, Cel6A) play key roles in the hydrolysis
of crystalline cellulose. CBH II from the white-rot
fungus Phanerochaete chrysosporium (PcCel6A) consists of
a catalytic domain (CD) and a carbohydrate-binding module
connected by a linker peptide, like other known fungal
cellobiohydrolases. In the present study, the CD of
PcCel6A was crystallized without ligands, and
p-nitrophenyl ß-d-cellotrioside (pNPG3) was soaked into
the crystals. The determined structures of the
ligand-free and pNPG3-soaked crystals revealed that
binding of cellobiose at substrate subsites +1 and +2
induces a conformational change of the N-terminal and
C-terminal loops, switching the tunnel-shaped active site
from the open to the closed form.The crystal structure of
the catalytic domain of a glycoside hydrolase family 6
cellobiohydrolase from the basidiomycete P. chrysosporium
was solved in apo and cellobiose-liganded forms at 1.2
and 2.1 Å resolution, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 398-403 |
| Journal | Acta Crystallographica Section F: Structural Biology Communications |
| Volume | 73 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2017 |
| MoE publication type | A1 Journal article-refereed |
Funding
This research was supported by a Grant-in-Aid for Innovative Areas from the Japanese Ministry of Education, Culture, Sports and Technology (MEXT) to KI (Nos. 24114001 and 24114008), a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) to KI (B, No. 24380089), an Impulsing Paradigm Change through Disruptive Technologies (ImPACT) from the Japan Science and Technology Agency (JST) to KI and a Grant-in-Aid for JSPS Fellows from JSPS to MT (15J10657).
Keywords
- biomass utilization
- carbohydrate-active enzymes
- cellobiohydrolase
- cellulases
- Phanerochaete chrysosporium
- Trisaccharides/chemistry
- Protein Conformation, alpha-Helical
- Catalytic Domain
- Gene Expression
- Nitrobenzenes/chemistry
- Phanerochaete/chemistry
- Models, Molecular
- Substrate Specificity
- Cellulose 1,4-beta-Cellobiosidase/chemistry
- Crystallography, X-Ray
- Pichia/genetics
- Amino Acid Motifs
- Recombinant Proteins/chemistry
- Protein Conformation, beta-Strand
- Fungal Proteins/chemistry
- Cloning, Molecular
- Hydrophobic and Hydrophilic Interactions
- Protein Binding
- Protein Interaction Domains and Motifs