Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5

T. Parkkinen (Corresponding Author), N. Hakulinen, Maija Tenkanen, Matti Siika-aho, Juha Rouvinen

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14 Citations (Scopus)

Abstract

Xylanase IV (XYN IV) is a new recently characterized xylanase from Trichoderma reesei. It is able to degrade several different xylans, mainly producing xylose. XYN IV has been crystallized by the hanging-drop vapour-diffusion method, using PEG 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 86.3, b = 137.5, c = 196.1 Å,  =  =  = 90°. Assuming a molecular weight of 50.3 kDa, the VM values indicate there to be four XYN IV monomers in an asymmetric unit and the solvent content of the crystals to be 57%. Based on dynamic light-scattering measurements, XYN IV is a dimer in solution. A native data set to 2.8 Å resolution has been collected at a home laboratory and a data set to 2.2 Å resolution has been collected using synchrotron radiation.
Original languageEnglish
Pages (from-to)542 - 544
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
VolumeD60
Issue number3
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

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Keywords

  • xylanases
  • glycoside hydrolase family 5
  • Trichoderma reesei

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