Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5

T. Parkkinen (Corresponding Author), N. Hakulinen, Maija Tenkanen, Matti Siika-aho, Juha Rouvinen

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

Xylanase IV (XYN IV) is a new recently characterized xylanase from Trichoderma reesei. It is able to degrade several different xylans, mainly producing xylose. XYN IV has been crystallized by the hanging-drop vapour-diffusion method, using PEG 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 86.3, b = 137.5, c = 196.1 Å,  =  =  = 90°. Assuming a molecular weight of 50.3 kDa, the VM values indicate there to be four XYN IV monomers in an asymmetric unit and the solvent content of the crystals to be 57%. Based on dynamic light-scattering measurements, XYN IV is a dimer in solution. A native data set to 2.8 Å resolution has been collected at a home laboratory and a data set to 2.2 Å resolution has been collected using synchrotron radiation.
Original languageEnglish
Pages (from-to)542 - 544
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
VolumeD60
Issue number3
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Glycoside Hydrolases
Crystallization
X-Rays
Xylans
Synchrotrons
Xylose
Molecular Weight
Radiation
Datasets
Polyethylene Glycol 6000
Dynamic Light Scattering

Keywords

  • xylanases
  • glycoside hydrolase family 5
  • Trichoderma reesei

Cite this

@article{947e64def602481fa0163cd842e2518d,
title = "Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5",
abstract = "Xylanase IV (XYN IV) is a new recently characterized xylanase from Trichoderma reesei. It is able to degrade several different xylans, mainly producing xylose. XYN IV has been crystallized by the hanging-drop vapour-diffusion method, using PEG 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 86.3, b = 137.5, c = 196.1 {\AA},  =  =  = 90°. Assuming a molecular weight of 50.3 kDa, the VM values indicate there to be four XYN IV monomers in an asymmetric unit and the solvent content of the crystals to be 57{\%}. Based on dynamic light-scattering measurements, XYN IV is a dimer in solution. A native data set to 2.8 {\AA} resolution has been collected at a home laboratory and a data set to 2.2 {\AA} resolution has been collected using synchrotron radiation.",
keywords = "xylanases, glycoside hydrolase family 5, Trichoderma reesei",
author = "T. Parkkinen and N. Hakulinen and Maija Tenkanen and Matti Siika-aho and Juha Rouvinen",
year = "2004",
doi = "10.1107/S0907444903029123",
language = "English",
volume = "D60",
pages = "542 -- 544",
journal = "Acta Crystallographica Section D: Structural Biology",
issn = "2059-7983",
publisher = "Wiley",
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}

Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5. / Parkkinen, T. (Corresponding Author); Hakulinen, N.; Tenkanen, Maija; Siika-aho, Matti; Rouvinen, Juha.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. D60, No. 3, 2004, p. 542 - 544.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5

AU - Parkkinen, T.

AU - Hakulinen, N.

AU - Tenkanen, Maija

AU - Siika-aho, Matti

AU - Rouvinen, Juha

PY - 2004

Y1 - 2004

N2 - Xylanase IV (XYN IV) is a new recently characterized xylanase from Trichoderma reesei. It is able to degrade several different xylans, mainly producing xylose. XYN IV has been crystallized by the hanging-drop vapour-diffusion method, using PEG 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 86.3, b = 137.5, c = 196.1 Å,  =  =  = 90°. Assuming a molecular weight of 50.3 kDa, the VM values indicate there to be four XYN IV monomers in an asymmetric unit and the solvent content of the crystals to be 57%. Based on dynamic light-scattering measurements, XYN IV is a dimer in solution. A native data set to 2.8 Å resolution has been collected at a home laboratory and a data set to 2.2 Å resolution has been collected using synchrotron radiation.

AB - Xylanase IV (XYN IV) is a new recently characterized xylanase from Trichoderma reesei. It is able to degrade several different xylans, mainly producing xylose. XYN IV has been crystallized by the hanging-drop vapour-diffusion method, using PEG 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 86.3, b = 137.5, c = 196.1 Å,  =  =  = 90°. Assuming a molecular weight of 50.3 kDa, the VM values indicate there to be four XYN IV monomers in an asymmetric unit and the solvent content of the crystals to be 57%. Based on dynamic light-scattering measurements, XYN IV is a dimer in solution. A native data set to 2.8 Å resolution has been collected at a home laboratory and a data set to 2.2 Å resolution has been collected using synchrotron radiation.

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KW - glycoside hydrolase family 5

KW - Trichoderma reesei

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DO - 10.1107/S0907444903029123

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JO - Acta Crystallographica Section D: Structural Biology

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