Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5

Elisabetta Sabini; Andrzej M. Brzozowski; Miroslawa Dauter; Gideon J. Davies; Keith S. Wilson; Marja Paloheimo; Pirkko Suominen; Matti Siika-Aho; Merja Penttilä

doi:10.1107/S0907444999002140

Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5

Elisabetta Sabini, Andrzej M. Brzozowski, Miroslawa Dauter, Gideon J. Davies, Keith S. Wilson, Marja Paloheimo, Pirkko Suominen, Matti Siika-Aho, Merja Penttilä

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3 Citations (Scopus)

Abstract

Crystals of the catalytic core domain of a Trichoderma reesei β-mannanase belonging to glycoside hydrolase family 5 have been grown by the sitting-drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P2₁, with unit-cell parameters a = 50.0, b = 54.3, c = 60.2 Å, β = 111.3°, and have a single monomer of mannanase in the asymmetric unit. Native data to 2.0 Å resolution have been collected at room temperature using synchrotron radiation. Data for a platinum derivative have been collected to 1.65 Å at 110 K in a very short time at the CCLRC Daresbury synchrotron source, using a charge-coupled device (CCD) as detector.

Original language	English
Pages (from-to)	1058-1060
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	55
Issue number	5
DOIs	https://doi.org/10.1107/S0907444999002140
Publication status	Published - 1 May 1999
MoE publication type	A1 Journal article-refereed

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Sabini, E., Brzozowski, A. M., Dauter, M., Davies, G. J., Wilson, K. S., Paloheimo, M., Suominen, P., Siika-Aho, M., & Penttilä, M. (1999). Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5. Acta Crystallographica Section D: Biological Crystallography, 55(5), 1058-1060. https://doi.org/10.1107/S0907444999002140

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title = "Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5",

abstract = "Crystals of the catalytic core domain of a Trichoderma reesei β-mannanase belonging to glycoside hydrolase family 5 have been grown by the sitting-drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P21, with unit-cell parameters a = 50.0, b = 54.3, c = 60.2 {\AA}, β = 111.3°, and have a single monomer of mannanase in the asymmetric unit. Native data to 2.0 {\AA} resolution have been collected at room temperature using synchrotron radiation. Data for a platinum derivative have been collected to 1.65 {\AA} at 110 K in a very short time at the CCLRC Daresbury synchrotron source, using a charge-coupled device (CCD) as detector.",

author = "Elisabetta Sabini and Brzozowski, {Andrzej M.} and Miroslawa Dauter and Davies, {Gideon J.} and Wilson, {Keith S.} and Marja Paloheimo and Pirkko Suominen and Matti Siika-Aho and Merja Penttil{\"a}",

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Sabini, E, Brzozowski, AM, Dauter, M, Davies, GJ, Wilson, KS, Paloheimo, M, Suominen, P, Siika-Aho, M & Penttilä, M 1999, 'Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5', Acta Crystallographica Section D: Biological Crystallography, vol. 55, no. 5, pp. 1058-1060. https://doi.org/10.1107/S0907444999002140

Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5. / Sabini, Elisabetta; Brzozowski, Andrzej M.; Dauter, Miroslawa et al.
In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 5, 01.05.1999, p. 1058-1060.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5

AU - Sabini, Elisabetta

AU - Brzozowski, Andrzej M.

AU - Dauter, Miroslawa

AU - Davies, Gideon J.

AU - Wilson, Keith S.

AU - Paloheimo, Marja

AU - Suominen, Pirkko

AU - Siika-Aho, Matti

AU - Penttilä, Merja

PY - 1999/5/1

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AB - Crystals of the catalytic core domain of a Trichoderma reesei β-mannanase belonging to glycoside hydrolase family 5 have been grown by the sitting-drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P21, with unit-cell parameters a = 50.0, b = 54.3, c = 60.2 Å, β = 111.3°, and have a single monomer of mannanase in the asymmetric unit. Native data to 2.0 Å resolution have been collected at room temperature using synchrotron radiation. Data for a platinum derivative have been collected to 1.65 Å at 110 K in a very short time at the CCLRC Daresbury synchrotron source, using a charge-coupled device (CCD) as detector.

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Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5

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