Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei

Terese Bergfors, Juha Rouvinen, Päivi Lehtovaara, Xavier Caldentey, Peter Tomme, Marc Claeyssens, Göran Pettersson, Tuula Teeri, Jonathan Knowles, Alwyn Jones

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

Single crystals of the core protein of the cellulase Cellobiohydrolase II have been grown in polyethylene glycol 6000 with the hanging drop method. Successful crystallization occurred only when 82 amino acids were removed from the N terminus by papain cleavage.
Original languageEnglish
Pages (from-to)167-169
JournalJournal of Molecular Biology
Volume209
Issue number1
DOIs
Publication statusPublished - 1989
MoE publication typeA1 Journal article-refereed

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Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Papain
Cellulase
Crystallization
Amino Acids
Proteins
Polyethylene Glycol 6000

Cite this

Bergfors, T., Rouvinen, J., Lehtovaara, P., Caldentey, X., Tomme, P., Claeyssens, M., ... Jones, A. (1989). Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei. Journal of Molecular Biology, 209(1), 167-169. https://doi.org/10.1016/0022-2836(89)90179-4
Bergfors, Terese ; Rouvinen, Juha ; Lehtovaara, Päivi ; Caldentey, Xavier ; Tomme, Peter ; Claeyssens, Marc ; Pettersson, Göran ; Teeri, Tuula ; Knowles, Jonathan ; Jones, Alwyn. / Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei. In: Journal of Molecular Biology. 1989 ; Vol. 209, No. 1. pp. 167-169.
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Bergfors, T, Rouvinen, J, Lehtovaara, P, Caldentey, X, Tomme, P, Claeyssens, M, Pettersson, G, Teeri, T, Knowles, J & Jones, A 1989, 'Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei', Journal of Molecular Biology, vol. 209, no. 1, pp. 167-169. https://doi.org/10.1016/0022-2836(89)90179-4

Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei. / Bergfors, Terese; Rouvinen, Juha; Lehtovaara, Päivi; Caldentey, Xavier; Tomme, Peter; Claeyssens, Marc; Pettersson, Göran; Teeri, Tuula; Knowles, Jonathan; Jones, Alwyn.

In: Journal of Molecular Biology, Vol. 209, No. 1, 1989, p. 167-169.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei

AU - Bergfors, Terese

AU - Rouvinen, Juha

AU - Lehtovaara, Päivi

AU - Caldentey, Xavier

AU - Tomme, Peter

AU - Claeyssens, Marc

AU - Pettersson, Göran

AU - Teeri, Tuula

AU - Knowles, Jonathan

AU - Jones, Alwyn

PY - 1989

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DO - 10.1016/0022-2836(89)90179-4

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Bergfors T, Rouvinen J, Lehtovaara P, Caldentey X, Tomme P, Claeyssens M et al. Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei. Journal of Molecular Biology. 1989;209(1):167-169. https://doi.org/10.1016/0022-2836(89)90179-4