Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei

Terese Bergfors; Juha Rouvinen; Päivi Lehtovaara; Xavier Caldentey; Peter Tomme; Marc Claeyssens; Göran Pettersson; Tuula Teeri; Jonathan Knowles; T. Alwyn Jones

doi:10.1016/0022-2836(89)90179-4

Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei

Terese Bergfors
, Juha Rouvinen
, Päivi Lehtovaara
, Xavier Caldentey
, Peter Tomme
, Marc Claeyssens
, Göran Pettersson
, Tuula Teeri
, Jonathan Knowles
, T. Alwyn Jones^*

^*Corresponding author for this work

VTT Technical Research Centre of Finland

Swedish University of Agricultural Sciences
Ghent University

Research output: Contribution to journal › Article › Scientific › peer-review

27 Citations (Scopus)

Abstract

Single crystals of the core protein of the cellulase Cellobiohydrolase II have been grown in polyethylene glycol 6000 with the hanging drop method. Successful crystallization occurred only when 82 amino acids were removed from the N terminus by papain cleavage.

Original language	English
Pages (from-to)	167-169
Journal	Journal of Molecular Biology
Volume	209
Issue number	1
DOIs	https://doi.org/10.1016/0022-2836(89)90179-4
Publication status	Published - 1989
MoE publication type	A1 Journal article-refereed

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@article{76eac9245f464a48b9a9fb5479ae3d76,

title = "Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei",

abstract = "Single crystals of the core protein of the cellulase Cellobiohydrolase II have been grown in polyethylene glycol 6000 with the hanging drop method. Successful crystallization occurred only when 82 amino acids were removed from the N terminus by papain cleavage.",

author = "Terese Bergfors and Juha Rouvinen and P{\"a}ivi Lehtovaara and Xavier Caldentey and Peter Tomme and Marc Claeyssens and G{\"o}ran Pettersson and Tuula Teeri and Jonathan Knowles and Jones, \{T. Alwyn\}",

year = "1989",

doi = "10.1016/0022-2836(89)90179-4",

language = "English",

volume = "209",

pages = "167--169",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

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T1 - Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei

AU - Bergfors, Terese

AU - Rouvinen, Juha

AU - Lehtovaara, Päivi

AU - Caldentey, Xavier

AU - Tomme, Peter

AU - Claeyssens, Marc

AU - Pettersson, Göran

AU - Teeri, Tuula

AU - Knowles, Jonathan

AU - Jones, T. Alwyn

PY - 1989

Y1 - 1989

N2 - Single crystals of the core protein of the cellulase Cellobiohydrolase II have been grown in polyethylene glycol 6000 with the hanging drop method. Successful crystallization occurred only when 82 amino acids were removed from the N terminus by papain cleavage.

AB - Single crystals of the core protein of the cellulase Cellobiohydrolase II have been grown in polyethylene glycol 6000 with the hanging drop method. Successful crystallization occurred only when 82 amino acids were removed from the N terminus by papain cleavage.

U2 - 10.1016/0022-2836(89)90179-4

DO - 10.1016/0022-2836(89)90179-4

M3 - Article

SN - 0022-2836

VL - 209

SP - 167

EP - 169

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Crystallization of the core protein of cellobiohydrolase II from Trichoderma reesei

Abstract

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