Culture conditions for efficient induction of green plants from isolated microspores of barley

Marjatta Salmenkallio-Marttila, Ulrika Kurten, Veli Kauppinen

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Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi.
A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169 +/- 97 green plants per spike were regenerated.
Original languageEnglish
Pages (from-to)79-81
JournalPlant Cell, Tissue and Organ Culture
Issue number1
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed


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