Culture conditions for efficient induction of green plants from isolated microspores of barley

Marjatta Salmenkallio-Marttila, Ulrika Kurten, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi.
A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169 +/- 97 green plants per spike were regenerated.
Original languageEnglish
Pages (from-to)79-81
JournalPlant Cell, Tissue and Organ Culture
Volume43
Issue number1
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

Fingerprint

microspores
barley
maltose
shortenings
Hordeum vulgare
plantlets
inflorescences
culture media
carbohydrates

Cite this

Salmenkallio-Marttila, Marjatta ; Kurten, Ulrika ; Kauppinen, Veli. / Culture conditions for efficient induction of green plants from isolated microspores of barley. In: Plant Cell, Tissue and Organ Culture. 1995 ; Vol. 43, No. 1. pp. 79-81.
@article{b36300e0c28b4ed7819e1d6d119006e2,
title = "Culture conditions for efficient induction of green plants from isolated microspores of barley",
abstract = "Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169 +/- 97 green plants per spike were regenerated.",
author = "Marjatta Salmenkallio-Marttila and Ulrika Kurten and Veli Kauppinen",
note = "Project code: BEL3030",
year = "1995",
doi = "10.1007/BF00042676",
language = "English",
volume = "43",
pages = "79--81",
journal = "Plant Cell, Tissue and Organ Culture",
issn = "0167-6857",
publisher = "Springer",
number = "1",

}

Culture conditions for efficient induction of green plants from isolated microspores of barley. / Salmenkallio-Marttila, Marjatta; Kurten, Ulrika; Kauppinen, Veli.

In: Plant Cell, Tissue and Organ Culture, Vol. 43, No. 1, 1995, p. 79-81.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Culture conditions for efficient induction of green plants from isolated microspores of barley

AU - Salmenkallio-Marttila, Marjatta

AU - Kurten, Ulrika

AU - Kauppinen, Veli

N1 - Project code: BEL3030

PY - 1995

Y1 - 1995

N2 - Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169 +/- 97 green plants per spike were regenerated.

AB - Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169 +/- 97 green plants per spike were regenerated.

U2 - 10.1007/BF00042676

DO - 10.1007/BF00042676

M3 - Article

VL - 43

SP - 79

EP - 81

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -