Culture conditions for efficient induction of green plants from isolated microspores of barley

Marjatta Salmenkallio-Marttila; Ulrika Kurtén; Veli Kauppinen

doi:10.1007/BF00042676

Culture conditions for efficient induction of green plants from isolated microspores of barley

Marjatta Salmenkallio-Marttila
, Ulrika Kurtén
, Veli Kauppinen

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

7 Citations (Scopus)

Abstract

Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169±97 green plants per spike were regenerated.

Original language	English
Pages (from-to)	79-81
Journal	Plant Cell, Tissue and Organ Culture
Volume	43
Issue number	1
DOIs	https://doi.org/10.1007/BF00042676
Publication status	Published - 1995
MoE publication type	A1 Journal article-refereed

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abstract = "Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169±97 green plants per spike were regenerated.",

author = "Marjatta Salmenkallio-Marttila and Ulrika Kurt{\'e}n and Veli Kauppinen",

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AU - Salmenkallio-Marttila, Marjatta

AU - Kurtén, Ulrika

AU - Kauppinen, Veli

N1 - Project code: BEL3030

PY - 1995

Y1 - 1995

N2 - Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169±97 green plants per spike were regenerated.

AB - Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169±97 green plants per spike were regenerated.

U2 - 10.1007/BF00042676

DO - 10.1007/BF00042676

M3 - Article

SN - 0167-6857

VL - 43

SP - 79

EP - 81

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 1

ER -

Culture conditions for efficient induction of green plants from isolated microspores of barley

Abstract

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