CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1

Zhengfu Zhou, Wei Zhang, Shiyou Su, Ming Chen, Wei Lu, Min Lin, István Molnár (Corresponding Author), Yuquan Xu (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.

Original languageEnglish
Pages (from-to)10539-10546
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume99
Issue number24
DOIs
Publication statusPublished - 1 Dec 2015
MoE publication typeA1 Journal article-refereed

Keywords

  • 2-β-hydroxylase
  • CYP287A1
  • Cytochrome P450
  • Deinococcus radiodurans
  • Deinoxanthin

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