CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1

Zhengfu Zhou; Wei Zhang; Shiyou Su; Ming Chen; Wei Lu; Min Lin; István Molnár; Yuquan Xu

doi:10.1007/s00253-015-6910-9

CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1

Zhengfu Zhou, Wei Zhang, Shiyou Su, Ming Chen, Wei Lu, Min Lin, István Molnár^*, Yuquan Xu^*

^*Corresponding author for this work

Not published at VTT

Research output: Contribution to journal › Article › Scientific › peer-review

19 Citations (Scopus)

Abstract

The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.

Original language	English
Pages (from-to)	10539-10546
Number of pages	8
Journal	Applied Microbiology and Biotechnology
Volume	99
Issue number	24
DOIs	https://doi.org/10.1007/s00253-015-6910-9
Publication status	Published - 1 Dec 2015
MoE publication type	A1 Journal article-refereed

Funding

This work was supported by grants from the National Basic Research (973) Program of China (2015CB755700), the National High-Tech (863) Program of China (No. 2012AA02A703), the National Research and Development Project of Transgenic Crops of China (2014ZX08009-003), the National Natural Science Foundation of China (No. 31170105), and the US National Science Foundation (MCB-0948751 to IM). This work was supported by grants from the National Basic Research (973) Program of China (2015CB755700), and the National High-Tech (863) Program of China (No. 2012AA02A703), and the National Research and Development Project of Transgenic Crops of China (2014ZX08009-003), the National Natural Science Foundation of China (No. 31170105), and the US National Science Foundation (MCB-0948751 to IM).

Keywords

2-β-hydroxylase
CYP287A1
Cytochrome P450
Deinococcus radiodurans
Deinoxanthin

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10.1007/s00253-015-6910-9

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title = "CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1",

abstract = "The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.",

keywords = "2-β-hydroxylase, CYP287A1, Cytochrome P450, Deinococcus radiodurans, Deinoxanthin",

author = "Zhengfu Zhou and Wei Zhang and Shiyou Su and Ming Chen and Wei Lu and Min Lin and Istv{\'a}n Moln{\'a}r and Yuquan Xu",

note = "Funding Information: This work was supported by grants from the National Basic Research (973) Program of China (2015CB755700), the National High-Tech (863) Program of China (No. 2012AA02A703), the National Research and Development Project of Transgenic Crops of China (2014ZX08009-003), the National Natural Science Foundation of China (No. 31170105), and the US National Science Foundation (MCB-0948751 to IM). Funding Information: This work was supported by grants from the National Basic Research (973) Program of China (2015CB755700), and the National High-Tech (863) Program of China (No. 2012AA02A703), and the National Research and Development Project of Transgenic Crops of China (2014ZX08009-003), the National Natural Science Foundation of China (No. 31170105), and the US National Science Foundation (MCB-0948751 to IM). Publisher Copyright: {\textcopyright} 2015, Springer-Verlag Berlin Heidelberg.",

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AU - Zhou, Zhengfu

AU - Zhang, Wei

AU - Su, Shiyou

AU - Chen, Ming

AU - Lu, Wei

AU - Lin, Min

AU - Molnár, István

AU - Xu, Yuquan

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N2 - The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.

AB - The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.

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CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1

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