Abstract
The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.
Original language | English |
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Pages (from-to) | 10539-10546 |
Number of pages | 8 |
Journal | Applied Microbiology and Biotechnology |
Volume | 99 |
Issue number | 24 |
DOIs | |
Publication status | Published - 1 Dec 2015 |
MoE publication type | A1 Journal article-refereed |
Funding
This work was supported by grants from the National Basic Research (973) Program of China (2015CB755700), the National High-Tech (863) Program of China (No. 2012AA02A703), the National Research and Development Project of Transgenic Crops of China (2014ZX08009-003), the National Natural Science Foundation of China (No. 31170105), and the US National Science Foundation (MCB-0948751 to IM). This work was supported by grants from the National Basic Research (973) Program of China (2015CB755700), and the National High-Tech (863) Program of China (No. 2012AA02A703), and the National Research and Development Project of Transgenic Crops of China (2014ZX08009-003), the National Natural Science Foundation of China (No. 31170105), and the US National Science Foundation (MCB-0948751 to IM).
Keywords
- 2-β-hydroxylase
- CYP287A1
- Cytochrome P450
- Deinococcus radiodurans
- Deinoxanthin