D-Tagatose production in the presence of borate by resting Lactococcus lactis cells harboring Bifidobacterium longum L-arabinose isomerase

N. Salonen (Corresponding Author), K. Salonen, M. Leisola, Antti Nyyssölä

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18 Citations (Scopus)

Abstract

Bifidobacterium longum NRRL B-41409 l-arabinose isomerase (l-AI) was overexpressed in Lactococcus lactis using a phosphate depletion inducible expression system. The resting L. lactis cells harboring the B. longum l-AI were used for production of d-tagatose from d-galactose in the presence of borate buffer. Multivariable analysis suggested that high pH, temperature and borate concentration favoured the conversion of d-galactose to d-tagatose. Almost quantitative conversion (92 %) was achieved at 20 g L−1 substrate and at 37.5 °C after 5 days. The d-tagatose production rate of 185 g L−1 day−1 was obtained at 300 g L−1 galactose, at 1.15 M borate, and at 41 °C during 10 days when the production medium was changed every 24 h. There was no significant loss in productivity during ten sequential 24 h batches. The initial d-tagatose production rate was 290 g L−1 day−1 under these conditions.
Original languageEnglish
Pages (from-to)489-497
Number of pages9
JournalBioprocess and Biosystems Engineering
Volume36
Issue number4
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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Keywords

  • Bifidobacterium longum
  • borate
  • d-tagatose
  • l-arabinose isomerase
  • Lactococcus lactis
  • resting cells

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