Day-length effects on protein localisation affect water absorption in barley ( Hordeum vulgare ) grains

Ulla R.M. Holopainen (Corresponding Author), Annika Wilhelmson, Silja Home, Kaisa Poutanen, Peter R Shewry

    Research output: Contribution to journalArticleScientificpeer-review

    11 Citations (Scopus)

    Abstract

    BACKGROUND: Hordeins are major storage proteins of barley (Hordeum vulgare L.) grains and are considered to influence malting and brewing by forming a matrix surrounding the starch granules which affects the release of fermentable sugars. However, the extent to which environmental factors affect hordein location, and the impact of this on malting performance, have not so far been studied. Therefore the relationship of hordein location to water uptake and malting quality were studied by growing barley cv. Barke under different daylengths (14 h and 18 h of light) in controlled environment conditions. RESULTS: Differences in the locations of hordein storage proteins were observed, with C hordein being located more deeply within the endosperm of both developing grains at 35 days after anthesis and in mature grains under long-day conditions. This deeper location of C hordein was correlated positively with water uptake during the steeping phase of malting. CONCLUSION: An effect of environment (daylength) on the localisation of C hordein was demonstrated. This difference in hordein localisation was also associated with differences in malting quality with water uptake in the steeping phase being associated positively with the deeper location of C hordein. These results indicate that environmental effects on protein location may affect malting performance of barley grains.
    Original languageEnglish
    Pages (from-to)2944-2951
    JournalJournal of the Science of Food and Agriculture
    Volume92
    Issue number15
    DOIs
    Publication statusPublished - Dec 2012
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Absorption
    • Glutens
    • Glutens: analysis
    • Hordeum
    • Hordeum: chemistry
    • Hordeum: growth & development
    • Hordeum: metabolism
    • Photoperiod
    • Seeds
    • Seeds: chemistry
    • Seeds: metabolism
    • Water
    • Water: metabolism

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