Day-length effects on protein localisation affect water absorption in barley ( Hordeum vulgare ) grains

Ulla R M Holopainen, Annika Wilhelmson, Silja Home, Kaisa Poutanen, Peter R Shewry

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

BACKGROUND: Hordeins are major storage proteins of barley (Hordeum vulgare L.) grains and are considered to influence malting and brewing by forming a matrix surrounding the starch granules which affects the release of fermentable sugars. However, the extent to which environmental factors affect hordein location, and the impact of this on malting performance, have not so far been studied. Therefore the relationship of hordein location to water uptake and malting quality were studied by growing barley cv. Barke under different daylengths (14 h and 18 h of light) in controlled environment conditions. RESULTS: Differences in the locations of hordein storage proteins were observed, with C hordein being located more deeply within the endosperm of both developing grains at 35 days after anthesis and in mature grains under long-day conditions. This deeper location of C hordein was correlated positively with water uptake during the steeping phase of malting. CONCLUSION: An effect of environment (daylength) on the localisation of C hordein was demonstrated. This difference in hordein localisation was also associated with differences in malting quality with water uptake in the steeping phase being associated positively with the deeper location of C hordein. These results indicate that environmental effects on protein location may affect malting performance of barley grains.
Original languageEnglish
Pages (from-to)2944-2951
Number of pages8
JournalJournal of the Science of Food and Agriculture
Volume92
Issue number15
DOIs
Publication statusPublished - Dec 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

hordein
Glutens
Hordeum
water uptake
Hordeum vulgare
photoperiod
barley
malting
Water
Proteins
proteins
malting quality
storage proteins
soaking
Controlled Environment
brewing
Endosperm
Water Quality
starch granules
endosperm

Keywords

  • Absorption
  • Glutens
  • Glutens: analysis
  • Hordeum
  • Hordeum: chemistry
  • Hordeum: growth & development
  • Hordeum: metabolism
  • Photoperiod
  • Seeds
  • Seeds: chemistry
  • Seeds: metabolism
  • Water
  • Water: metabolism

Cite this

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title = "Day-length effects on protein localisation affect water absorption in barley ( Hordeum vulgare ) grains",
abstract = "BACKGROUND: Hordeins are major storage proteins of barley (Hordeum vulgare L.) grains and are considered to influence malting and brewing by forming a matrix surrounding the starch granules which affects the release of fermentable sugars. However, the extent to which environmental factors affect hordein location, and the impact of this on malting performance, have not so far been studied. Therefore the relationship of hordein location to water uptake and malting quality were studied by growing barley cv. Barke under different daylengths (14 h and 18 h of light) in controlled environment conditions. RESULTS: Differences in the locations of hordein storage proteins were observed, with C hordein being located more deeply within the endosperm of both developing grains at 35 days after anthesis and in mature grains under long-day conditions. This deeper location of C hordein was correlated positively with water uptake during the steeping phase of malting. CONCLUSION: An effect of environment (daylength) on the localisation of C hordein was demonstrated. This difference in hordein localisation was also associated with differences in malting quality with water uptake in the steeping phase being associated positively with the deeper location of C hordein. These results indicate that environmental effects on protein location may affect malting performance of barley grains.",
keywords = "Absorption, Glutens, Glutens: analysis, Hordeum, Hordeum: chemistry, Hordeum: growth & development, Hordeum: metabolism, Photoperiod, Seeds, Seeds: chemistry, Seeds: metabolism, Water, Water: metabolism",
author = "Holopainen, {Ulla R M} and Annika Wilhelmson and Silja Home and Kaisa Poutanen and Shewry, {Peter R}",
year = "2012",
month = "12",
doi = "10.1002/jsfa.5706",
language = "English",
volume = "92",
pages = "2944--2951",
journal = "Journal of the Science of Food and Agriculture",
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}

Day-length effects on protein localisation affect water absorption in barley ( Hordeum vulgare ) grains. / Holopainen, Ulla R M; Wilhelmson, Annika; Home, Silja; Poutanen, Kaisa; Shewry, Peter R.

In: Journal of the Science of Food and Agriculture, Vol. 92, No. 15, 12.2012, p. 2944-2951.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Day-length effects on protein localisation affect water absorption in barley ( Hordeum vulgare ) grains

AU - Holopainen, Ulla R M

AU - Wilhelmson, Annika

AU - Home, Silja

AU - Poutanen, Kaisa

AU - Shewry, Peter R

PY - 2012/12

Y1 - 2012/12

N2 - BACKGROUND: Hordeins are major storage proteins of barley (Hordeum vulgare L.) grains and are considered to influence malting and brewing by forming a matrix surrounding the starch granules which affects the release of fermentable sugars. However, the extent to which environmental factors affect hordein location, and the impact of this on malting performance, have not so far been studied. Therefore the relationship of hordein location to water uptake and malting quality were studied by growing barley cv. Barke under different daylengths (14 h and 18 h of light) in controlled environment conditions. RESULTS: Differences in the locations of hordein storage proteins were observed, with C hordein being located more deeply within the endosperm of both developing grains at 35 days after anthesis and in mature grains under long-day conditions. This deeper location of C hordein was correlated positively with water uptake during the steeping phase of malting. CONCLUSION: An effect of environment (daylength) on the localisation of C hordein was demonstrated. This difference in hordein localisation was also associated with differences in malting quality with water uptake in the steeping phase being associated positively with the deeper location of C hordein. These results indicate that environmental effects on protein location may affect malting performance of barley grains.

AB - BACKGROUND: Hordeins are major storage proteins of barley (Hordeum vulgare L.) grains and are considered to influence malting and brewing by forming a matrix surrounding the starch granules which affects the release of fermentable sugars. However, the extent to which environmental factors affect hordein location, and the impact of this on malting performance, have not so far been studied. Therefore the relationship of hordein location to water uptake and malting quality were studied by growing barley cv. Barke under different daylengths (14 h and 18 h of light) in controlled environment conditions. RESULTS: Differences in the locations of hordein storage proteins were observed, with C hordein being located more deeply within the endosperm of both developing grains at 35 days after anthesis and in mature grains under long-day conditions. This deeper location of C hordein was correlated positively with water uptake during the steeping phase of malting. CONCLUSION: An effect of environment (daylength) on the localisation of C hordein was demonstrated. This difference in hordein localisation was also associated with differences in malting quality with water uptake in the steeping phase being associated positively with the deeper location of C hordein. These results indicate that environmental effects on protein location may affect malting performance of barley grains.

KW - Absorption

KW - Glutens

KW - Glutens: analysis

KW - Hordeum

KW - Hordeum: chemistry

KW - Hordeum: growth & development

KW - Hordeum: metabolism

KW - Photoperiod

KW - Seeds

KW - Seeds: chemistry

KW - Seeds: metabolism

KW - Water

KW - Water: metabolism

U2 - 10.1002/jsfa.5706

DO - 10.1002/jsfa.5706

M3 - Article

VL - 92

SP - 2944

EP - 2951

JO - Journal of the Science of Food and Agriculture

JF - Journal of the Science of Food and Agriculture

SN - 0022-5142

IS - 15

ER -