Abstract
The marine-derived Scopulariopsis brevicaulis strain
LF580 produces scopularides A and B, which have
anticancerous properties. We carried out genome
sequencing using three next-generation DNA sequencing
methods. De novo hybrid assembly yielded 621 scaffolds
with a total size of 32.2 Mb and 16298 putative gene
models. We identified a large non-ribosomal peptide
synthetase gene (nrps1) and supporting pks2 gene in the
same biosynthetic gene cluster. This cluster and the
genes within the cluster are functionally active as
confirmed by RNA-Seq. Characterization of
carbohydrate-active enzymes and major facilitator
superfamily (MFS)-type transporters lead to postulate S.
brevicaulis originated from a soil fungus, which came
into contact with the marine sponge Tethya aurantium.
This marine sponge seems to provide shelter to this
fungus and micro-environment suitable for its survival in
the ocean. This study also builds the platform for
further investigations of the role of life-style and
secondary metabolites from S. brevicaulis.
Original language | English |
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Article number | e0140398 |
Journal | PLoS ONE |
Volume | 10 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2015 |
MoE publication type | A1 Journal article-refereed |