The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA sequencing methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb and 16298 putative gene models. We identified a large non-ribosomal peptide synthetase gene (nrps1) and supporting pks2 gene in the same biosynthetic gene cluster. This cluster and the genes within the cluster are functionally active as confirmed by RNA-Seq. Characterization of carbohydrate-active enzymes and major facilitator superfamily (MFS)-type transporters lead to postulate S. brevicaulis originated from a soil fungus, which came into contact with the marine sponge Tethya aurantium. This marine sponge seems to provide shelter to this fungus and micro-environment suitable for its survival in the ocean. This study also builds the platform for further investigations of the role of life-style and secondary metabolites from S. brevicaulis.
Kumar, A., Henrissat, B., Arvas, M., Syed, M. F., Thieme, N., Benz, J. P., Sørensen, J. L., Record, E., Pöggeler, S., & Kempken, F. (2015). De novo assembly and genome analyses of the marine-derived Scopulariopsis brevicaulis strain LF580 unravels life-style traits and anticancerous scopularide biosynthetic gene cluster. PLoS ONE, 10(10), [e0140398]. https://doi.org/10.1371/journal.pone.0140398