De novo assembly and genome analyses of the marine-derived Scopulariopsis brevicaulis strain LF580 unravels life-style traits and anticancerous scopularide biosynthetic gene cluster

Abhishek Kumar, Bernard Henrissat, Mikko Arvas, Muhammad Fahad Syed, Nils Thieme, J. Philipp Benz, Jens Laurids Sørensen, Eric Record, Stefanie Pöggeler, Frank Kempken

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Abstract

The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA sequencing methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb and 16298 putative gene models. We identified a large non-ribosomal peptide synthetase gene (nrps1) and supporting pks2 gene in the same biosynthetic gene cluster. This cluster and the genes within the cluster are functionally active as confirmed by RNA-Seq. Characterization of carbohydrate-active enzymes and major facilitator superfamily (MFS)-type transporters lead to postulate S. brevicaulis originated from a soil fungus, which came into contact with the marine sponge Tethya aurantium. This marine sponge seems to provide shelter to this fungus and micro-environment suitable for its survival in the ocean. This study also builds the platform for further investigations of the role of life-style and secondary metabolites from S. brevicaulis.
Original languageEnglish
Article numbere0140398
JournalPLoS ONE
Volume10
Issue number10
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

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Kumar, A., Henrissat, B., Arvas, M., Syed, M. F., Thieme, N., Benz, J. P., Sørensen, J. L., Record, E., Pöggeler, S., & Kempken, F. (2015). De novo assembly and genome analyses of the marine-derived Scopulariopsis brevicaulis strain LF580 unravels life-style traits and anticancerous scopularide biosynthetic gene cluster. PLoS ONE, 10(10), [e0140398]. https://doi.org/10.1371/journal.pone.0140398