Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks

A. Gutiérrez (Corresponding Author), J. Rencoret, E. M. Cadena, A. Rico, Dorothee Barth, J. C. del Rio, T. T. Martinez

Research output: Contribution to journalArticleScientificpeer-review

84 Citations (Scopus)

Abstract

The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g−1 laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment.
Original languageEnglish
Pages (from-to)114-122
Number of pages8
JournalBioresource Technology
Volume119
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Lignin
Feedstocks
lignin
Wood
Demonstrations
Polysaccharides
Dimethyl Sulfoxide
polysaccharide
Glucose
nuclear magnetic resonance
ethanol
glucose
Ethanol
Enzymes
Nuclear magnetic resonance
enzyme
Acids
removal
acid

Keywords

  • Enzymatic deconstruction
  • laccase
  • bioethanol
  • lignocellulose
  • 2D NMR

Cite this

Gutiérrez, A., Rencoret, J., Cadena, E. M., Rico, A., Barth, D., del Rio, J. C., & Martinez, T. T. (2012). Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks. Bioresource Technology, 119, 114-122. https://doi.org/10.1016/j.biortech.2012.05.112
Gutiérrez, A. ; Rencoret, J. ; Cadena, E. M. ; Rico, A. ; Barth, Dorothee ; del Rio, J. C. ; Martinez, T. T. / Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks. In: Bioresource Technology. 2012 ; Vol. 119. pp. 114-122.
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abstract = "The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g−1 laccase and 2.5{\%} HBT concentration, 48{\%} and 32{\%} of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61{\%} and 12{\%} in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment.",
keywords = "Enzymatic deconstruction, laccase, bioethanol, lignocellulose, 2D NMR",
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Gutiérrez, A, Rencoret, J, Cadena, EM, Rico, A, Barth, D, del Rio, JC & Martinez, TT 2012, 'Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks', Bioresource Technology, vol. 119, pp. 114-122. https://doi.org/10.1016/j.biortech.2012.05.112

Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks. / Gutiérrez, A. (Corresponding Author); Rencoret, J.; Cadena, E. M.; Rico, A.; Barth, Dorothee; del Rio, J. C.; Martinez, T. T.

In: Bioresource Technology, Vol. 119, 2012, p. 114-122.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks

AU - Gutiérrez, A.

AU - Rencoret, J.

AU - Cadena, E. M.

AU - Rico, A.

AU - Barth, Dorothee

AU - del Rio, J. C.

AU - Martinez, T. T.

PY - 2012

Y1 - 2012

N2 - The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g−1 laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment.

AB - The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g−1 laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment.

KW - Enzymatic deconstruction

KW - laccase

KW - bioethanol

KW - lignocellulose

KW - 2D NMR

U2 - 10.1016/j.biortech.2012.05.112

DO - 10.1016/j.biortech.2012.05.112

M3 - Article

VL - 119

SP - 114

EP - 122

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -