Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling

Kristiina Kruus (Corresponding Author), Atte Mikkelson, Tarja Tamminen, Matti Siika-aho, Jaakko Pere, Fernando Jose Borges Gomes, Jorge Luiz Colodette

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Abstract

Adsorption of xylan on pulp is a potential method to improve its properties, especially refinability for high quality printing and writing (P&W) paper grades. In this study, elephant grass [ Pennisetum purpureum (Schumach.)] xylan was used for this purpose. The xylan was extracted using cold caustic extraction (CCE) from P. purpureum brown pulp, produced by the Soda-AQ process (kappa 20). Xylan resorption was accomplished during the oxygen delignification phase of eucalypt [ Eucalyptus globulus (Labill.)] pulp to avoid problems induced by the colour of the lignin-contaminated deposited xylan. Immunolabelling and enzymatic peeling methodologies were compared for the analysis of the spatial distribution of xylan in the fibre wall. The labelling appeared unevenly as faint and brighter patches on fibre surfaces. Increased labelling of xylan was detected on the samples with precipitated P. purpureum xylan. The enzymatic peeling method using a total hydrolysis enzyme mixture yielded a composition gradient as a function of time, showing clear xylose (Xyl) enrichment in the very beginning of the reaction, reflecting hydrolysis of fibre surfaces. Pure xylanase and endoglucanase hydrolyses yielded different product patterns and kinetics compared to total hydrolysis, but interpretation of those results in terms of xylan localisation was not straightforward. Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling | Request PDF. Available from: https://www.researchgate.net/publication/327595575_Deposition_of_xylan_isolated_from_Pennisetum_purpureum_on_fibres_of_Eucalyptus_globulus_and_characterisation_of_the_composition_of_the_surface_xylans_by_immunolabelling_and_enzymatic_peeling [accessed Sep 18 2018].
Original languageEnglish
Pages (from-to)915-922
Number of pages8
JournalHolzforschung
Volume72
Issue number11
Early online dateJul 2018
DOIs
Publication statusPublished - 1 Nov 2018
MoE publication typeNot Eligible

Fingerprint

Xylans
Peeling
Fibers
Chemical analysis
Hydrolysis
Pulp
Labeling
Caustics
Delignification
Xylose
Cellulase
Lignin
Spatial distribution
Printing

Keywords

  • elephant grass
  • enzymatic hydrolysis
  • Eucalyptus globulus
  • fibre surface composition
  • immunolabelling

Cite this

@article{bf2893d1e5264cb3adcea6eb4b595620,
title = "Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling",
abstract = "Adsorption of xylan on pulp is a potential method to improve its properties, especially refinability for high quality printing and writing (P&W) paper grades. In this study, elephant grass [ Pennisetum purpureum (Schumach.)] xylan was used for this purpose. The xylan was extracted using cold caustic extraction (CCE) from P. purpureum brown pulp, produced by the Soda-AQ process (kappa 20). Xylan resorption was accomplished during the oxygen delignification phase of eucalypt [ Eucalyptus globulus (Labill.)] pulp to avoid problems induced by the colour of the lignin-contaminated deposited xylan. Immunolabelling and enzymatic peeling methodologies were compared for the analysis of the spatial distribution of xylan in the fibre wall. The labelling appeared unevenly as faint and brighter patches on fibre surfaces. Increased labelling of xylan was detected on the samples with precipitated P. purpureum xylan. The enzymatic peeling method using a total hydrolysis enzyme mixture yielded a composition gradient as a function of time, showing clear xylose (Xyl) enrichment in the very beginning of the reaction, reflecting hydrolysis of fibre surfaces. Pure xylanase and endoglucanase hydrolyses yielded different product patterns and kinetics compared to total hydrolysis, but interpretation of those results in terms of xylan localisation was not straightforward. Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling | Request PDF. Available from: https://www.researchgate.net/publication/327595575_Deposition_of_xylan_isolated_from_Pennisetum_purpureum_on_fibres_of_Eucalyptus_globulus_and_characterisation_of_the_composition_of_the_surface_xylans_by_immunolabelling_and_enzymatic_peeling [accessed Sep 18 2018].",
keywords = "elephant grass, enzymatic hydrolysis, Eucalyptus globulus, fibre surface composition, immunolabelling",
author = "Kristiina Kruus and Atte Mikkelson and Tarja Tamminen and Matti Siika-aho and Jaakko Pere and Gomes, {Fernando Jose Borges} and Colodette, {Jorge Luiz}",
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language = "English",
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TY - JOUR

T1 - Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling

AU - Kruus, Kristiina

AU - Mikkelson, Atte

AU - Tamminen, Tarja

AU - Siika-aho, Matti

AU - Pere, Jaakko

AU - Gomes, Fernando Jose Borges

AU - Colodette, Jorge Luiz

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Adsorption of xylan on pulp is a potential method to improve its properties, especially refinability for high quality printing and writing (P&W) paper grades. In this study, elephant grass [ Pennisetum purpureum (Schumach.)] xylan was used for this purpose. The xylan was extracted using cold caustic extraction (CCE) from P. purpureum brown pulp, produced by the Soda-AQ process (kappa 20). Xylan resorption was accomplished during the oxygen delignification phase of eucalypt [ Eucalyptus globulus (Labill.)] pulp to avoid problems induced by the colour of the lignin-contaminated deposited xylan. Immunolabelling and enzymatic peeling methodologies were compared for the analysis of the spatial distribution of xylan in the fibre wall. The labelling appeared unevenly as faint and brighter patches on fibre surfaces. Increased labelling of xylan was detected on the samples with precipitated P. purpureum xylan. The enzymatic peeling method using a total hydrolysis enzyme mixture yielded a composition gradient as a function of time, showing clear xylose (Xyl) enrichment in the very beginning of the reaction, reflecting hydrolysis of fibre surfaces. Pure xylanase and endoglucanase hydrolyses yielded different product patterns and kinetics compared to total hydrolysis, but interpretation of those results in terms of xylan localisation was not straightforward. Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling | Request PDF. Available from: https://www.researchgate.net/publication/327595575_Deposition_of_xylan_isolated_from_Pennisetum_purpureum_on_fibres_of_Eucalyptus_globulus_and_characterisation_of_the_composition_of_the_surface_xylans_by_immunolabelling_and_enzymatic_peeling [accessed Sep 18 2018].

AB - Adsorption of xylan on pulp is a potential method to improve its properties, especially refinability for high quality printing and writing (P&W) paper grades. In this study, elephant grass [ Pennisetum purpureum (Schumach.)] xylan was used for this purpose. The xylan was extracted using cold caustic extraction (CCE) from P. purpureum brown pulp, produced by the Soda-AQ process (kappa 20). Xylan resorption was accomplished during the oxygen delignification phase of eucalypt [ Eucalyptus globulus (Labill.)] pulp to avoid problems induced by the colour of the lignin-contaminated deposited xylan. Immunolabelling and enzymatic peeling methodologies were compared for the analysis of the spatial distribution of xylan in the fibre wall. The labelling appeared unevenly as faint and brighter patches on fibre surfaces. Increased labelling of xylan was detected on the samples with precipitated P. purpureum xylan. The enzymatic peeling method using a total hydrolysis enzyme mixture yielded a composition gradient as a function of time, showing clear xylose (Xyl) enrichment in the very beginning of the reaction, reflecting hydrolysis of fibre surfaces. Pure xylanase and endoglucanase hydrolyses yielded different product patterns and kinetics compared to total hydrolysis, but interpretation of those results in terms of xylan localisation was not straightforward. Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling | Request PDF. Available from: https://www.researchgate.net/publication/327595575_Deposition_of_xylan_isolated_from_Pennisetum_purpureum_on_fibres_of_Eucalyptus_globulus_and_characterisation_of_the_composition_of_the_surface_xylans_by_immunolabelling_and_enzymatic_peeling [accessed Sep 18 2018].

KW - elephant grass

KW - enzymatic hydrolysis

KW - Eucalyptus globulus

KW - fibre surface composition

KW - immunolabelling

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U2 - 10.1515/hf-2018-0002

DO - 10.1515/hf-2018-0002

M3 - Article

VL - 72

SP - 915

EP - 922

JO - Holzforschung

JF - Holzforschung

SN - 0018-3830

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ER -