The novel genus Holtermanniella is proposed here to accommodate four Cryptococcus species closely related to Holtermannia corniformis that are included in the Holtermannia clade (Basidiomycota, Agaricomycotina). Thus, four novel combinations are proposed: Holtermanniella nyarrowii comb. nov., Holtermanniella festucosa comb. nov., Holtermanniella mycelialis comb. nov. and Holtermanniella wattica
comb. nov. In addition, a novel anamorphic yeast species was studied
with 15 isolates obtained from different habitats around the world.
Analysis of the sequences of the D1/D2 region of their large subunit
rDNA showed that the novel species is placed phylogenetically within the
Holtermannia clade of the
Tremellomycetes (Agaricomycotina, Basidiomycota). PCR fingerprinting and
sequencing of ITS1–5.8S–ITS2 showed genetic intraspecific variability
among the strains: three groups were formed, which did not correlate
with geographical origin or substrate. This novel species, designated
the type species of Holtermanniella gen. nov., is described as Holtermanniella takashimae sp. nov.; the type strain is CBS 11174T (=HB 982T =DBVPG 8012T). The order Holtermanniales ord. nov. is proposed here to include Holtermannia (the type genus) and Holtermanniella.