Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip

Santeri Tuomikoski (Corresponding Author), Nina Virkkala, Stella Rovio, Ari Hokkanen, Heli Sirén, Sami Franssila

    Research output: Contribution to journalArticleScientificpeer-review

    21 Citations (Scopus)


    Integrated solid-phase extraction-zone electrophoresis (SPE-ZE) device has been designed and fabricated on microchip. The structures were fabricated by using multiple layers of SU-8 polymer with a novel technique that enables easy alignment and high yield of the chips. SU-8 adhesive bonding has two major advantages: it enables bonding of high aspect ratio pillars and it results in fully SU-8 microchannels with uniform electrokinetic flow properties. The SPE-ZE device has a fluidic reservoir with 15:1 high aspect ratio pillars for bead filters that act as a SPE part in the chip structure. The separation unit is a 25 mm long electrophoresis channel starting from the outlet of SPE reservoir. Argon laser-induced fluorescence (LIF) detector was used to monitor simultaneously the SPE reservoir and the detection site at the end of the electrophoresis channel. Flow characteristics and electric field distributions were simulated with Femlab software. Fluorescein was used as the analyte for detecting the operational performance of the chip. Adsorption, bead rinsing, elution and detection were tested to verify functioning of the chip design.
    Original languageEnglish
    Pages (from-to)258-266
    Number of pages9
    JournalJournal of Chromatography A
    Issue number2
    Publication statusPublished - 2006
    MoE publication typeA1 Journal article-refereed
    Event18th International Symposium on MicroScale Bioseparations - New Orleans, United States
    Duration: 12 Feb 200517 Feb 2005


    • solid phase extraction
    • electrophoresis
    • microchip
    • SU-8
    • microchannel


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