Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip

Santeri Tuomikoski (Corresponding Author), Nina Virkkala, Stella Rovio, Ari Hokkanen, Heli Sirén, Sami Franssila

    Research output: Contribution to journalArticleScientificpeer-review

    20 Citations (Scopus)

    Abstract

    Integrated solid-phase extraction-zone electrophoresis (SPE-ZE) device has been designed and fabricated on microchip. The structures were fabricated by using multiple layers of SU-8 polymer with a novel technique that enables easy alignment and high yield of the chips. SU-8 adhesive bonding has two major advantages: it enables bonding of high aspect ratio pillars and it results in fully SU-8 microchannels with uniform electrokinetic flow properties. The SPE-ZE device has a fluidic reservoir with 15:1 high aspect ratio pillars for bead filters that act as a SPE part in the chip structure. The separation unit is a 25 mm long electrophoresis channel starting from the outlet of SPE reservoir. Argon laser-induced fluorescence (LIF) detector was used to monitor simultaneously the SPE reservoir and the detection site at the end of the electrophoresis channel. Flow characteristics and electric field distributions were simulated with Femlab software. Fluorescein was used as the analyte for detecting the operational performance of the chip. Adsorption, bead rinsing, elution and detection were tested to verify functioning of the chip design.
    Original languageEnglish
    Pages (from-to)258-266
    Number of pages9
    JournalJournal of Chromatography A
    Volume1111
    Issue number2
    DOIs
    Publication statusPublished - 2006
    MoE publication typeA1 Journal article-refereed
    Event18th International Symposium on MicroScale Bioseparations - New Orleans, United States
    Duration: 12 Feb 200517 Feb 2005

    Fingerprint

    Microchip Electrophoresis
    Solid Phase Extraction
    Electrophoresis
    Fabrication
    Aspect ratio
    Argon lasers
    Equipment and Supplies
    Argon
    Fluidics
    Microchannels
    Fluorescein
    Adhesives
    Adsorption
    Polymers
    Lasers
    Software
    Fluorescence
    Electric fields
    Detectors

    Keywords

    • solid phase extraction
    • electrophoresis
    • microchip
    • SU-8
    • microchannel

    Cite this

    Tuomikoski, Santeri ; Virkkala, Nina ; Rovio, Stella ; Hokkanen, Ari ; Sirén, Heli ; Franssila, Sami. / Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip. In: Journal of Chromatography A. 2006 ; Vol. 1111, No. 2. pp. 258-266.
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    abstract = "Integrated solid-phase extraction-zone electrophoresis (SPE-ZE) device has been designed and fabricated on microchip. The structures were fabricated by using multiple layers of SU-8 polymer with a novel technique that enables easy alignment and high yield of the chips. SU-8 adhesive bonding has two major advantages: it enables bonding of high aspect ratio pillars and it results in fully SU-8 microchannels with uniform electrokinetic flow properties. The SPE-ZE device has a fluidic reservoir with 15:1 high aspect ratio pillars for bead filters that act as a SPE part in the chip structure. The separation unit is a 25 mm long electrophoresis channel starting from the outlet of SPE reservoir. Argon laser-induced fluorescence (LIF) detector was used to monitor simultaneously the SPE reservoir and the detection site at the end of the electrophoresis channel. Flow characteristics and electric field distributions were simulated with Femlab software. Fluorescein was used as the analyte for detecting the operational performance of the chip. Adsorption, bead rinsing, elution and detection were tested to verify functioning of the chip design.",
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    author = "Santeri Tuomikoski and Nina Virkkala and Stella Rovio and Ari Hokkanen and Heli Sir{\'e}n and Sami Franssila",
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    Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip. / Tuomikoski, Santeri (Corresponding Author); Virkkala, Nina; Rovio, Stella; Hokkanen, Ari; Sirén, Heli; Franssila, Sami.

    In: Journal of Chromatography A, Vol. 1111, No. 2, 2006, p. 258-266.

    Research output: Contribution to journalArticleScientificpeer-review

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    T1 - Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip

    AU - Tuomikoski, Santeri

    AU - Virkkala, Nina

    AU - Rovio, Stella

    AU - Hokkanen, Ari

    AU - Sirén, Heli

    AU - Franssila, Sami

    PY - 2006

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    AB - Integrated solid-phase extraction-zone electrophoresis (SPE-ZE) device has been designed and fabricated on microchip. The structures were fabricated by using multiple layers of SU-8 polymer with a novel technique that enables easy alignment and high yield of the chips. SU-8 adhesive bonding has two major advantages: it enables bonding of high aspect ratio pillars and it results in fully SU-8 microchannels with uniform electrokinetic flow properties. The SPE-ZE device has a fluidic reservoir with 15:1 high aspect ratio pillars for bead filters that act as a SPE part in the chip structure. The separation unit is a 25 mm long electrophoresis channel starting from the outlet of SPE reservoir. Argon laser-induced fluorescence (LIF) detector was used to monitor simultaneously the SPE reservoir and the detection site at the end of the electrophoresis channel. Flow characteristics and electric field distributions were simulated with Femlab software. Fluorescein was used as the analyte for detecting the operational performance of the chip. Adsorption, bead rinsing, elution and detection were tested to verify functioning of the chip design.

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    KW - electrophoresis

    KW - microchip

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    KW - microchannel

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