Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip

Santeri Tuomikoski (Corresponding Author), Nina Virkkala, Stella Rovio, Ari Hokkanen, Heli Sirén, Sami Franssila

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

Integrated solid-phase extraction-zone electrophoresis (SPE-ZE) device has been designed and fabricated on microchip. The structures were fabricated by using multiple layers of SU-8 polymer with a novel technique that enables easy alignment and high yield of the chips. SU-8 adhesive bonding has two major advantages: it enables bonding of high aspect ratio pillars and it results in fully SU-8 microchannels with uniform electrokinetic flow properties. The SPE-ZE device has a fluidic reservoir with 15:1 high aspect ratio pillars for bead filters that act as a SPE part in the chip structure. The separation unit is a 25 mm long electrophoresis channel starting from the outlet of SPE reservoir. Argon laser-induced fluorescence (LIF) detector was used to monitor simultaneously the SPE reservoir and the detection site at the end of the electrophoresis channel. Flow characteristics and electric field distributions were simulated with Femlab software. Fluorescein was used as the analyte for detecting the operational performance of the chip. Adsorption, bead rinsing, elution and detection were tested to verify functioning of the chip design.
Original languageEnglish
Pages (from-to)258-266
Number of pages9
JournalJournal of Chromatography A
Volume1111
Issue number2
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed
Event18th International Symposium on MicroScale Bioseparations - New Orleans, United States
Duration: 12 Feb 200517 Feb 2005

Fingerprint

Microchip Electrophoresis
Solid Phase Extraction
Electrophoresis
Fabrication
Aspect ratio
Argon lasers
Equipment and Supplies
Argon
Fluidics
Microchannels
Fluorescein
Adhesives
Adsorption
Polymers
Lasers
Software
Fluorescence
Electric fields
Detectors

Keywords

  • solid phase extraction
  • electrophoresis
  • microchip
  • SU-8
  • microchannel

Cite this

Tuomikoski, Santeri ; Virkkala, Nina ; Rovio, Stella ; Hokkanen, Ari ; Sirén, Heli ; Franssila, Sami. / Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip. In: Journal of Chromatography A. 2006 ; Vol. 1111, No. 2. pp. 258-266.
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abstract = "Integrated solid-phase extraction-zone electrophoresis (SPE-ZE) device has been designed and fabricated on microchip. The structures were fabricated by using multiple layers of SU-8 polymer with a novel technique that enables easy alignment and high yield of the chips. SU-8 adhesive bonding has two major advantages: it enables bonding of high aspect ratio pillars and it results in fully SU-8 microchannels with uniform electrokinetic flow properties. The SPE-ZE device has a fluidic reservoir with 15:1 high aspect ratio pillars for bead filters that act as a SPE part in the chip structure. The separation unit is a 25 mm long electrophoresis channel starting from the outlet of SPE reservoir. Argon laser-induced fluorescence (LIF) detector was used to monitor simultaneously the SPE reservoir and the detection site at the end of the electrophoresis channel. Flow characteristics and electric field distributions were simulated with Femlab software. Fluorescein was used as the analyte for detecting the operational performance of the chip. Adsorption, bead rinsing, elution and detection were tested to verify functioning of the chip design.",
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Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip. / Tuomikoski, Santeri (Corresponding Author); Virkkala, Nina; Rovio, Stella; Hokkanen, Ari; Sirén, Heli; Franssila, Sami.

In: Journal of Chromatography A, Vol. 1111, No. 2, 2006, p. 258-266.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Tuomikoski, Santeri

AU - Virkkala, Nina

AU - Rovio, Stella

AU - Hokkanen, Ari

AU - Sirén, Heli

AU - Franssila, Sami

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AB - Integrated solid-phase extraction-zone electrophoresis (SPE-ZE) device has been designed and fabricated on microchip. The structures were fabricated by using multiple layers of SU-8 polymer with a novel technique that enables easy alignment and high yield of the chips. SU-8 adhesive bonding has two major advantages: it enables bonding of high aspect ratio pillars and it results in fully SU-8 microchannels with uniform electrokinetic flow properties. The SPE-ZE device has a fluidic reservoir with 15:1 high aspect ratio pillars for bead filters that act as a SPE part in the chip structure. The separation unit is a 25 mm long electrophoresis channel starting from the outlet of SPE reservoir. Argon laser-induced fluorescence (LIF) detector was used to monitor simultaneously the SPE reservoir and the detection site at the end of the electrophoresis channel. Flow characteristics and electric field distributions were simulated with Femlab software. Fluorescein was used as the analyte for detecting the operational performance of the chip. Adsorption, bead rinsing, elution and detection were tested to verify functioning of the chip design.

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KW - electrophoresis

KW - microchip

KW - SU-8

KW - microchannel

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