Abstract
Integrated SPE-CE microfluidistic chip has been designed
and fabricated. Fabrication process has been optimized to
produce high yield from the SU-8 process. Process
innovations in adhesive wafer bonding and in division of
double SU-8 layer exposure reduced alignment errors and
reduced stresses in the structures. Achieved yield was
75% after process optimization. Fluidic and electrical
behaviour of the chip has been simulated. Pillar-type
bead filter shows uniform fluidic flow. Beads in the
whole reservoir are applied for extraction. From the
simulations it can be noticed that electric field behaves
uniformly in the reservoir and therefore the
electrokinetic transportation from the reservoir to the
CE-channel occurs as desired. Fluidic tests have been
made with testosterone samples. Successful extraction,
elution and detection have been performed with the
fluidic chips. However, size and shape of the
SPE-reservoir should be optimized to performhigh
efficiency separations in both directions with SPE-CE
device. This can be done by further simulations and
experiments. SU-8 process developed here can be easily
adapted to the requirements of the fluidic performance.
Both lateral dimensions and depth of the structures can
be easily adjusted in the SU-8 process.
Original language | English |
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Title of host publication | 18th International Symposium on Micro-Scale Bioseparations |
Subtitle of host publication | MBS'2005 Symposium |
Pages | 198 |
Number of pages | 1 |
Publication status | Published - 2005 |
Event | 18th International Symposium on Micro-Scale Bioseparations, MBS 2005 - New Orleans, United States Duration: 12 Feb 2005 → 17 Feb 2005 |
Conference
Conference | 18th International Symposium on Micro-Scale Bioseparations, MBS 2005 |
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Abbreviated title | MBS 2005 |
Country/Territory | United States |
City | New Orleans |
Period | 12/02/05 → 17/02/05 |
Keywords
- microchip
- fabrication
- solid-phase extraction
- testosterone
- electrophoresis