Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations

Muhammad Mujtaba (Corresponding Author), Murat Kaya, Lalehan Akyuz, Demet Erdonmez, Bahar Akyuz, Idris Sargin

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Current study was designed to use the newly obtained cellulose from waste flower spikes of Thypa latifolia plant for plasmid DNA adsorption. Cellulose was isolated according to a previously described method including acid and base treatment, and cellulose content was recorded as 17%. T. latifolia cellulose was physicochemically characterized via FT-IR, TGA and SEM techniques. Detailed mechanism of plasmid DNA adsorption by newly isolated cellulose was described using chemical quantum calculations. To check the effect of Cu++ immobilization on the affinity of cellulose for plasmid DNA, copper ions were immobilized onto T. latifolia cellulose. pUC18 plasmid DNA was used for adsorption studies. Membranes prepared with only T. latifolia cellulose and Cu++ immobilized T. latifolia cellulose revealed different adsorption ratios as 43.9 and 86.9% respectively. This newly isolated cellulose from waste flower spikes of T. latifolia can be utilized as a suitable carrier for plasmid DNA.

Original languageEnglish
Pages (from-to)914-923
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume102
DOIs
Publication statusPublished - 1 Sep 2017
MoE publication typeA1 Journal article-refereed

Keywords

  • Cu Immobilization
  • FT-IR
  • pUC18
  • SEM
  • TGA

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