Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations

Muhammad Mujtaba; Murat Kaya; Lalehan Akyuz; Demet Erdonmez; Bahar Akyuz; Idris Sargin

doi:10.1016/j.ijbiomac.2017.04.104

Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations

Muhammad Mujtaba^* (Corresponding Author), Murat Kaya, Lalehan Akyuz, Demet Erdonmez, Bahar Akyuz, Idris Sargin

^*Corresponding author for this work

Not published at VTT

Aksaray University

Research output: Contribution to journal › Article › Scientific › peer-review

7 Citations (Scopus)

Abstract

Current study was designed to use the newly obtained cellulose from waste flower spikes of Thypa latifolia plant for plasmid DNA adsorption. Cellulose was isolated according to a previously described method including acid and base treatment, and cellulose content was recorded as 17%. T. latifolia cellulose was physicochemically characterized via FT-IR, TGA and SEM techniques. Detailed mechanism of plasmid DNA adsorption by newly isolated cellulose was described using chemical quantum calculations. To check the effect of Cu⁺⁺ immobilization on the affinity of cellulose for plasmid DNA, copper ions were immobilized onto T. latifolia cellulose. pUC18 plasmid DNA was used for adsorption studies. Membranes prepared with only T. latifolia cellulose and Cu⁺⁺ immobilized T. latifolia cellulose revealed different adsorption ratios as 43.9 and 86.9% respectively. This newly isolated cellulose from waste flower spikes of T. latifolia can be utilized as a suitable carrier for plasmid DNA.

Original language	English
Pages (from-to)	914-923
Number of pages	10
Journal	International Journal of Biological Macromolecules
Volume	102
DOIs	https://doi.org/10.1016/j.ijbiomac.2017.04.104
Publication status	Published - 1 Sept 2017
MoE publication type	A1 Journal article-refereed

Keywords

Cu Immobilization
FT-IR
pUC18
SEM
TGA

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10.1016/j.ijbiomac.2017.04.104

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title = "Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations",

abstract = "Current study was designed to use the newly obtained cellulose from waste flower spikes of Thypa latifolia plant for plasmid DNA adsorption. Cellulose was isolated according to a previously described method including acid and base treatment, and cellulose content was recorded as 17%. T. latifolia cellulose was physicochemically characterized via FT-IR, TGA and SEM techniques. Detailed mechanism of plasmid DNA adsorption by newly isolated cellulose was described using chemical quantum calculations. To check the effect of Cu++ immobilization on the affinity of cellulose for plasmid DNA, copper ions were immobilized onto T. latifolia cellulose. pUC18 plasmid DNA was used for adsorption studies. Membranes prepared with only T. latifolia cellulose and Cu++ immobilized T. latifolia cellulose revealed different adsorption ratios as 43.9 and 86.9% respectively. This newly isolated cellulose from waste flower spikes of T. latifolia can be utilized as a suitable carrier for plasmid DNA.",

keywords = "Cu Immobilization, FT-IR, pUC18, SEM, TGA",

author = "Muhammad Mujtaba and Murat Kaya and Lalehan Akyuz and Demet Erdonmez and Bahar Akyuz and Idris Sargin",

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year = "2017",

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doi = "10.1016/j.ijbiomac.2017.04.104",

language = "English",

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Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations. / Mujtaba, Muhammad (Corresponding Author); Kaya, Murat; Akyuz, Lalehan et al.
In: International Journal of Biological Macromolecules, Vol. 102, 01.09.2017, p. 914-923.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations

AU - Mujtaba, Muhammad

AU - Kaya, Murat

AU - Akyuz, Lalehan

AU - Erdonmez, Demet

AU - Akyuz, Bahar

AU - Sargin, Idris

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Current study was designed to use the newly obtained cellulose from waste flower spikes of Thypa latifolia plant for plasmid DNA adsorption. Cellulose was isolated according to a previously described method including acid and base treatment, and cellulose content was recorded as 17%. T. latifolia cellulose was physicochemically characterized via FT-IR, TGA and SEM techniques. Detailed mechanism of plasmid DNA adsorption by newly isolated cellulose was described using chemical quantum calculations. To check the effect of Cu++ immobilization on the affinity of cellulose for plasmid DNA, copper ions were immobilized onto T. latifolia cellulose. pUC18 plasmid DNA was used for adsorption studies. Membranes prepared with only T. latifolia cellulose and Cu++ immobilized T. latifolia cellulose revealed different adsorption ratios as 43.9 and 86.9% respectively. This newly isolated cellulose from waste flower spikes of T. latifolia can be utilized as a suitable carrier for plasmid DNA.

AB - Current study was designed to use the newly obtained cellulose from waste flower spikes of Thypa latifolia plant for plasmid DNA adsorption. Cellulose was isolated according to a previously described method including acid and base treatment, and cellulose content was recorded as 17%. T. latifolia cellulose was physicochemically characterized via FT-IR, TGA and SEM techniques. Detailed mechanism of plasmid DNA adsorption by newly isolated cellulose was described using chemical quantum calculations. To check the effect of Cu++ immobilization on the affinity of cellulose for plasmid DNA, copper ions were immobilized onto T. latifolia cellulose. pUC18 plasmid DNA was used for adsorption studies. Membranes prepared with only T. latifolia cellulose and Cu++ immobilized T. latifolia cellulose revealed different adsorption ratios as 43.9 and 86.9% respectively. This newly isolated cellulose from waste flower spikes of T. latifolia can be utilized as a suitable carrier for plasmid DNA.

KW - Cu Immobilization

KW - FT-IR

KW - pUC18

KW - SEM

KW - TGA

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SN - 0141-8130

VL - 102

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EP - 923

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations

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Keywords

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