Detection and molecular characterization of filamentous actinobacteria and thermoactinomycetes present in water-damaged building materials

Maija-Liisa Suihko, Outi Priha (Corresponding Author), Hanna-Leena Alakomi, Peter Thompson, Björn Mälarstig, Richard Stott, Malcolm Richardson

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

In this study the dominant filamentous actinobacteria occurring in water‐damaged building materials were detected by culture and characterized by automated ribotyping and 16S rRNA gene sequencing. Fifty‐two samples were taken from 20 water‐damaged houses in four different countries. A total of 122 bacterial isolates were analyzed. Actinobacteria or thermoactinomycetes were present in 48% of the samples. The dominant genus was Streptomyces (58% of isolates), followed by Thermoactinomyces (23%), Laceyella (14%), Nocardiopsis (3%), Pseudonocardia (1%) and Saccharomonospora (1%). The most frequently detected species was the thermophilic Thermoactinomyces vulgaris (14 samples/4 countries). The most common streptomycetes were closely related to the heterogeneous species Streptomyces microflavus (7/2) or Streptomyces griseus (6/2). Automated ribotyping was a rapid tool for reliable characterization of these isolates. The spores of thermoactinomycetes and toxic substances of Nocardiopsis species and S. griseus may constitute a risk for human health.
Original languageEnglish
Pages (from-to)268-277
Number of pages10
JournalIndoor Air
Volume19
Issue number3
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Keywords

  • water-damaged buildings
  • actinobacteria
  • thermoactinomycetes
  • automated ribotyping
  • 16S rDNA

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