Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 339-344 |
| Number of pages | 6 |
| Journal | Journal of the American Society of Brewing Chemists |
| Volume | 75 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2017 |
| MoE publication type | A1 Journal article-refereed |
Fingerprint
Keywords
- gene variance
- hop-tolerance gene
- horA
- lactic acid bacteria
- lactobacillus
- pediococcus
- protein modeling
Cite this
}
Detection of a hop-tolerance gene horA insertion variant in lactic acid bacteria that results in a truncated horA lacking the walker B motif necessary for transport function. / Bergsveinson, Jordyn; Kajala, Ilkka; Goerzen, Scott; Ziola, Barry.
In: Journal of the American Society of Brewing Chemists, Vol. 75, No. 4, 2017, p. 339-344.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Detection of a hop-tolerance gene horA insertion variant in lactic acid bacteria that results in a truncated horA lacking the walker B motif necessary for transport function
AU - Bergsveinson, Jordyn
AU - Kajala, Ilkka
AU - Goerzen, Scott
AU - Ziola, Barry
PY - 2017
Y1 - 2017
N2 - The hop-tolerance gene horA frequently found in beer-spoilage lactic acid bacteria (LAB) was investigated for sequence variability. Although the horA gene was found to have less sequence variability relative to the LAB hop-tolerance gene horC, a sequence insertion in horA in some isolates resulted in early truncation of HorA translation. This truncated HorA was found in LAB both capable and incapable of growth in beer. Protein modeling revealed that the truncated HorA may retain some capacity to bind and sequester hop iso-a-acids but lacks the transport function essential for moving hop compounds out of the cell. Sequence analysis of LAB plasmids that contain horA revealed a high level of conservation in all of the genes comprising the horA gene cassette. Assessing whether a LAB isolated in a brewery setting is capable of making a full-length protein with intact hop transport function or a truncated HorA requires redesign of commonly used horA polymerase chain reaction primers to target detection of horA both with and without the sequence insertion.
AB - The hop-tolerance gene horA frequently found in beer-spoilage lactic acid bacteria (LAB) was investigated for sequence variability. Although the horA gene was found to have less sequence variability relative to the LAB hop-tolerance gene horC, a sequence insertion in horA in some isolates resulted in early truncation of HorA translation. This truncated HorA was found in LAB both capable and incapable of growth in beer. Protein modeling revealed that the truncated HorA may retain some capacity to bind and sequester hop iso-a-acids but lacks the transport function essential for moving hop compounds out of the cell. Sequence analysis of LAB plasmids that contain horA revealed a high level of conservation in all of the genes comprising the horA gene cassette. Assessing whether a LAB isolated in a brewery setting is capable of making a full-length protein with intact hop transport function or a truncated HorA requires redesign of commonly used horA polymerase chain reaction primers to target detection of horA both with and without the sequence insertion.
KW - gene variance
KW - hop-tolerance gene
KW - horA
KW - lactic acid bacteria
KW - lactobacillus
KW - pediococcus
KW - protein modeling
UR - http://www.scopus.com/inward/record.url?scp=85039444361&partnerID=8YFLogxK
U2 - 10.1094/ASBCJ-2017-4682-01
DO - 10.1094/ASBCJ-2017-4682-01
M3 - Article
VL - 75
SP - 339
EP - 344
JO - Journal of the American Society of Brewing Chemists
JF - Journal of the American Society of Brewing Chemists
SN - 0361-0470
IS - 4
ER -