Detection of a hop-tolerance gene horA insertion variant in lactic acid bacteria that results in a truncated horA lacking the walker B motif necessary for transport function

Jordyn Bergsveinson, Ilkka Kajala, Scott Goerzen, Barry Ziola

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The hop-tolerance gene horA frequently found in beer-spoilage lactic acid bacteria (LAB) was investigated for sequence variability. Although the horA gene was found to have less sequence variability relative to the LAB hop-tolerance gene horC, a sequence insertion in horA in some isolates resulted in early truncation of HorA translation. This truncated HorA was found in LAB both capable and incapable of growth in beer. Protein modeling revealed that the truncated HorA may retain some capacity to bind and sequester hop iso-a-acids but lacks the transport function essential for moving hop compounds out of the cell. Sequence analysis of LAB plasmids that contain horA revealed a high level of conservation in all of the genes comprising the horA gene cassette. Assessing whether a LAB isolated in a brewery setting is capable of making a full-length protein with intact hop transport function or a truncated HorA requires redesign of commonly used horA polymerase chain reaction primers to target detection of horA both with and without the sequence insertion.
Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalJournal of the American Society of Brewing Chemists
Volume75
Issue number4
DOIs
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Humulus
Insertional Mutagenesis
hops
lactic acid bacteria
Lactic Acid
Bacteria
Genes
beers
genes
brewing industry
spoilage
transposons
translation (genetics)
Sequence Analysis
plasmids
Proteins
Plasmids
sequence analysis
proteins
polymerase chain reaction

Keywords

  • gene variance
  • hop-tolerance gene
  • horA
  • lactic acid bacteria
  • lactobacillus
  • pediococcus
  • protein modeling

Cite this

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title = "Detection of a hop-tolerance gene horA insertion variant in lactic acid bacteria that results in a truncated horA lacking the walker B motif necessary for transport function",
abstract = "The hop-tolerance gene horA frequently found in beer-spoilage lactic acid bacteria (LAB) was investigated for sequence variability. Although the horA gene was found to have less sequence variability relative to the LAB hop-tolerance gene horC, a sequence insertion in horA in some isolates resulted in early truncation of HorA translation. This truncated HorA was found in LAB both capable and incapable of growth in beer. Protein modeling revealed that the truncated HorA may retain some capacity to bind and sequester hop iso-a-acids but lacks the transport function essential for moving hop compounds out of the cell. Sequence analysis of LAB plasmids that contain horA revealed a high level of conservation in all of the genes comprising the horA gene cassette. Assessing whether a LAB isolated in a brewery setting is capable of making a full-length protein with intact hop transport function or a truncated HorA requires redesign of commonly used horA polymerase chain reaction primers to target detection of horA both with and without the sequence insertion.",
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author = "Jordyn Bergsveinson and Ilkka Kajala and Scott Goerzen and Barry Ziola",
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journal = "Journal of the American Society of Brewing Chemists",
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Detection of a hop-tolerance gene horA insertion variant in lactic acid bacteria that results in a truncated horA lacking the walker B motif necessary for transport function. / Bergsveinson, Jordyn; Kajala, Ilkka; Goerzen, Scott; Ziola, Barry.

In: Journal of the American Society of Brewing Chemists, Vol. 75, No. 4, 2017, p. 339-344.

Research output: Contribution to journalArticleScientificpeer-review

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