Detection of bacterial contamination in starch and resin-based papermaking chemicals using fluorescencce techniques

Liisa Nohynek (Corresponding Author), Eija Saski, Auli Haikara, Laura Raaska

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

Rapid fluorescence techniques were evaluated for the detection of bacterial contaminants in papermaking chemicals including starch and the resin-based sizes and starch slurries used in the paper industry. Viable and non-viable bacterial cells were visualised by fluorescent probes and detected by epifluorescence microscopy and flow cytometry. The best discrimination ability was obtained with the fluorescent probes LIVE/DEAD and SYBR Green, based on the staining of cellular nucleic acid, and ChemChrome V3, which demonstrated cellular enzymatic activity. The process samples had to be diluted and filtered before fluorescence staining and analysis because they were viscous and contained solid particles. Fluorescence microscopic counts of bacteria in highly contaminated process samples were similar to plate counts, but flow cytometric enumeration of bacterial cells in process samples yielded 2- to 10-fold lower counts compared with plate counts, depending on the consistency of the sample. The detection limits in flow cytometric analysis and in epifluorescence microscopy were 103–106 cells ml−1 and 105–106 cells ml−1, respectively. Intrinsic bacterial contamination was detectable with fluorescence techniques and highly contaminated process samples could be analysed with fluorescence methods.
Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalJournal of industrial microbiology and biotechnology
Volume30
Issue number4
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

Papermaking
Starch
Contamination
Resins
Fluorescence
Fluorescent Dyes
Microscopy
Microscopic examination
Staining and Labeling
Flow cytometry
Nucleic acids
Slurries
Nucleic Acids
Limit of Detection
Bacteria
Industry
Flow Cytometry
Impurities

Keywords

  • Starch
  • Fluorescence techniques
  • Paper industry
  • Hygiene

Cite this

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title = "Detection of bacterial contamination in starch and resin-based papermaking chemicals using fluorescencce techniques",
abstract = "Rapid fluorescence techniques were evaluated for the detection of bacterial contaminants in papermaking chemicals including starch and the resin-based sizes and starch slurries used in the paper industry. Viable and non-viable bacterial cells were visualised by fluorescent probes and detected by epifluorescence microscopy and flow cytometry. The best discrimination ability was obtained with the fluorescent probes LIVE/DEAD and SYBR Green, based on the staining of cellular nucleic acid, and ChemChrome V3, which demonstrated cellular enzymatic activity. The process samples had to be diluted and filtered before fluorescence staining and analysis because they were viscous and contained solid particles. Fluorescence microscopic counts of bacteria in highly contaminated process samples were similar to plate counts, but flow cytometric enumeration of bacterial cells in process samples yielded 2- to 10-fold lower counts compared with plate counts, depending on the consistency of the sample. The detection limits in flow cytometric analysis and in epifluorescence microscopy were 103–106 cells ml−1 and 105–106 cells ml−1, respectively. Intrinsic bacterial contamination was detectable with fluorescence techniques and highly contaminated process samples could be analysed with fluorescence methods.",
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Detection of bacterial contamination in starch and resin-based papermaking chemicals using fluorescencce techniques. / Nohynek, Liisa (Corresponding Author); Saski, Eija; Haikara, Auli; Raaska, Laura.

In: Journal of industrial microbiology and biotechnology, Vol. 30, No. 4, 2003, p. 239-244.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

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AU - Saski, Eija

AU - Haikara, Auli

AU - Raaska, Laura

PY - 2003

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N2 - Rapid fluorescence techniques were evaluated for the detection of bacterial contaminants in papermaking chemicals including starch and the resin-based sizes and starch slurries used in the paper industry. Viable and non-viable bacterial cells were visualised by fluorescent probes and detected by epifluorescence microscopy and flow cytometry. The best discrimination ability was obtained with the fluorescent probes LIVE/DEAD and SYBR Green, based on the staining of cellular nucleic acid, and ChemChrome V3, which demonstrated cellular enzymatic activity. The process samples had to be diluted and filtered before fluorescence staining and analysis because they were viscous and contained solid particles. Fluorescence microscopic counts of bacteria in highly contaminated process samples were similar to plate counts, but flow cytometric enumeration of bacterial cells in process samples yielded 2- to 10-fold lower counts compared with plate counts, depending on the consistency of the sample. The detection limits in flow cytometric analysis and in epifluorescence microscopy were 103–106 cells ml−1 and 105–106 cells ml−1, respectively. Intrinsic bacterial contamination was detectable with fluorescence techniques and highly contaminated process samples could be analysed with fluorescence methods.

AB - Rapid fluorescence techniques were evaluated for the detection of bacterial contaminants in papermaking chemicals including starch and the resin-based sizes and starch slurries used in the paper industry. Viable and non-viable bacterial cells were visualised by fluorescent probes and detected by epifluorescence microscopy and flow cytometry. The best discrimination ability was obtained with the fluorescent probes LIVE/DEAD and SYBR Green, based on the staining of cellular nucleic acid, and ChemChrome V3, which demonstrated cellular enzymatic activity. The process samples had to be diluted and filtered before fluorescence staining and analysis because they were viscous and contained solid particles. Fluorescence microscopic counts of bacteria in highly contaminated process samples were similar to plate counts, but flow cytometric enumeration of bacterial cells in process samples yielded 2- to 10-fold lower counts compared with plate counts, depending on the consistency of the sample. The detection limits in flow cytometric analysis and in epifluorescence microscopy were 103–106 cells ml−1 and 105–106 cells ml−1, respectively. Intrinsic bacterial contamination was detectable with fluorescence techniques and highly contaminated process samples could be analysed with fluorescence methods.

KW - Starch

KW - Fluorescence techniques

KW - Paper industry

KW - Hygiene

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