Detection of DNA and poly-l-lysine using CVD graphene-channel FET biosensors

Aniket Kakatkar, T. S. Abhilash, R. De Alba, J. M. Parpia, H. G. Craighead

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

A graphene channel field-effect biosensor is demonstrated for detecting the binding of double-stranded DNA and poly-l-lysine. Sensors consist of chemical vapor deposition graphene transferred using a clean, etchant-free transfer method. The presence of DNA and poly-l-lysine are detected by the conductance change of the graphene transistor. A readily measured shift in the Dirac voltage (the voltage at which the graphene's resistance peaks) is observed after the graphene channel is exposed to solutions containing DNA or poly-l-lysine. The 'Dirac voltage shift' is attributed to the binding/unbinding of charged molecules on the graphene surface. The polarity of the response changes to positive direction with poly-l-lysine and negative direction with DNA. This response results in detection limits of 8 pM for 48.5 kbp DNA and 11 pM for poly-l-lysine. The biosensors are easy to fabricate, reusable and are promising as sensors of a wide variety of charged biomolecules.

Original languageEnglish
Article number125502
JournalNanotechnology
Volume26
Issue number12
DOIs
Publication statusPublished - 27 Mar 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Graphite
Field effect transistors
Biosensors
Graphene
Lysine
Chemical vapor deposition
DNA
Electric potential
Sensors
Biomolecules
Molecules

Keywords

  • biosensor
  • field effect transistors
  • grapheme

Cite this

Kakatkar, Aniket ; Abhilash, T. S. ; De Alba, R. ; Parpia, J. M. ; Craighead, H. G. / Detection of DNA and poly-l-lysine using CVD graphene-channel FET biosensors. In: Nanotechnology. 2015 ; Vol. 26, No. 12.
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Detection of DNA and poly-l-lysine using CVD graphene-channel FET biosensors. / Kakatkar, Aniket; Abhilash, T. S.; De Alba, R.; Parpia, J. M.; Craighead, H. G.

In: Nanotechnology, Vol. 26, No. 12, 125502, 27.03.2015.

Research output: Contribution to journalArticleScientificpeer-review

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