Detection of DNA and poly-l-lysine using CVD graphene-channel FET biosensors

Aniket Kakatkar, T. S. Abhilash, R. De Alba, J. M. Parpia, H. G. Craighead

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

A graphene channel field-effect biosensor is demonstrated for detecting the binding of double-stranded DNA and poly-l-lysine. Sensors consist of chemical vapor deposition graphene transferred using a clean, etchant-free transfer method. The presence of DNA and poly-l-lysine are detected by the conductance change of the graphene transistor. A readily measured shift in the Dirac voltage (the voltage at which the graphene's resistance peaks) is observed after the graphene channel is exposed to solutions containing DNA or poly-l-lysine. The 'Dirac voltage shift' is attributed to the binding/unbinding of charged molecules on the graphene surface. The polarity of the response changes to positive direction with poly-l-lysine and negative direction with DNA. This response results in detection limits of 8 pM for 48.5 kbp DNA and 11 pM for poly-l-lysine. The biosensors are easy to fabricate, reusable and are promising as sensors of a wide variety of charged biomolecules.

Original languageEnglish
Article number125502
JournalNanotechnology
Volume26
Issue number12
DOIs
Publication statusPublished - 27 Mar 2015
MoE publication typeA1 Journal article-refereed

Keywords

  • biosensor
  • field effect transistors
  • grapheme

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