Detection of Pectinatus contaminants in beer

Auli Haikara

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The suitability of membrane filtration for detecting Pectinatus cells in filtered beer was investigated. The average recovery of Pectinatus cells was 5–19%, depending on the physiological state of the cells. Obviously, this method cannot be recommended for detecting Pectinatus in filtered beer samples. The reason for the low recovery is that most old cells, corresponding to stressed cells in practice, died during membrane filtration. The use of prereduced media or a carbon dioxide atmosphere during the procedure did not improve the recovery significantly. After forcing, direct staining of cells on the membrane by the immunofluorescence technique or by the direct epifluorescent filter technique with acridine orange gave satisfactory results. Low contamination levels (10–100 cells) could be detected after three to four days when active cells were used. With old cells, a forcing time of four to five days was needed. Direct staining revealed contamination two to four days before the beer became visually turbid. This method can be used for detecting Pectinatus contamination in different stages of bottling.

Original languageEnglish
Pages (from-to)43 - 46
Number of pages4
JournalJournal of the American Society of Brewing Chemists
Volume43
Issue number1
DOIs
Publication statusPublished - 1985
MoE publication typeNot Eligible

Fingerprint

Pectinatus
beers
cells
Staining and Labeling
Direct Fluorescent Antibody Technique
Acridine Orange
bottling
Membranes
acridine orange
Atmosphere
Carbon Dioxide
physiological state
methodology
fluorescent antibody technique
Cell Membrane

Keywords

  • Pectinatus

Cite this

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title = "Detection of Pectinatus contaminants in beer",
abstract = "The suitability of membrane filtration for detecting Pectinatus cells in filtered beer was investigated. The average recovery of Pectinatus cells was 5–19{\%}, depending on the physiological state of the cells. Obviously, this method cannot be recommended for detecting Pectinatus in filtered beer samples. The reason for the low recovery is that most old cells, corresponding to stressed cells in practice, died during membrane filtration. The use of prereduced media or a carbon dioxide atmosphere during the procedure did not improve the recovery significantly. After forcing, direct staining of cells on the membrane by the immunofluorescence technique or by the direct epifluorescent filter technique with acridine orange gave satisfactory results. Low contamination levels (10–100 cells) could be detected after three to four days when active cells were used. With old cells, a forcing time of four to five days was needed. Direct staining revealed contamination two to four days before the beer became visually turbid. This method can be used for detecting Pectinatus contamination in different stages of bottling.",
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Detection of Pectinatus contaminants in beer. / Haikara, Auli.

In: Journal of the American Society of Brewing Chemists, Vol. 43, No. 1, 1985, p. 43 - 46.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Detection of Pectinatus contaminants in beer

AU - Haikara, Auli

PY - 1985

Y1 - 1985

N2 - The suitability of membrane filtration for detecting Pectinatus cells in filtered beer was investigated. The average recovery of Pectinatus cells was 5–19%, depending on the physiological state of the cells. Obviously, this method cannot be recommended for detecting Pectinatus in filtered beer samples. The reason for the low recovery is that most old cells, corresponding to stressed cells in practice, died during membrane filtration. The use of prereduced media or a carbon dioxide atmosphere during the procedure did not improve the recovery significantly. After forcing, direct staining of cells on the membrane by the immunofluorescence technique or by the direct epifluorescent filter technique with acridine orange gave satisfactory results. Low contamination levels (10–100 cells) could be detected after three to four days when active cells were used. With old cells, a forcing time of four to five days was needed. Direct staining revealed contamination two to four days before the beer became visually turbid. This method can be used for detecting Pectinatus contamination in different stages of bottling.

AB - The suitability of membrane filtration for detecting Pectinatus cells in filtered beer was investigated. The average recovery of Pectinatus cells was 5–19%, depending on the physiological state of the cells. Obviously, this method cannot be recommended for detecting Pectinatus in filtered beer samples. The reason for the low recovery is that most old cells, corresponding to stressed cells in practice, died during membrane filtration. The use of prereduced media or a carbon dioxide atmosphere during the procedure did not improve the recovery significantly. After forcing, direct staining of cells on the membrane by the immunofluorescence technique or by the direct epifluorescent filter technique with acridine orange gave satisfactory results. Low contamination levels (10–100 cells) could be detected after three to four days when active cells were used. With old cells, a forcing time of four to five days was needed. Direct staining revealed contamination two to four days before the beer became visually turbid. This method can be used for detecting Pectinatus contamination in different stages of bottling.

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