Detection of: Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Sanna Uusitalo, Martin Kögler, Anna Liisa Välimaa, Alexey P. Popov, Yu V. Ryabchikov, Ville Kontturi, Samuli Siitonen, Jarno Petäjä, T. Virtanen, R. Laitinen, Matti Kinnunen, I. V. Meglinski, Andrei V. Kabashin, Alex Bunker, Tapani J. S. Viitala, Jussi A. Hiltunen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been studied for the detection of foodborne microbes. The majority of the approaches are either based on costly patterning techniques on silicon or glass wafers or on methods which have not been tested in large scale fabrication. We demonstrate the feasibility of analyte specific sensing using mass-produced, polymer-based low-cost SERS substrate in analysing the chosen model microbe with biological recognition. The use of this novel roll-to-roll fabricated SERS substrate was combined with optimised gold nanoparticles to increase the detection sensitivity. Distinctive SERS spectral bands were recorded for Listeria innocua ATCC 33090 using an in-house build (785 nm) near infra red (NIR) Raman system. Results were compared to both those found in the literature and the results obtained from a commercial time-gated Raman system with a 532 nm wavelength laser excitation. The effect of the SERS enhancer metal and the excitation wavelength on the detected spectra was found to be negligible. The hypothesis that disagreements within the literature regarding bacterial spectra results from conditions present during the detection process has not been supported. The sensitivity of our SERS detection was improved through optimization of the concentration of the sample inside the hydrophobic polydimethylsiloxane (PDMS) wells. Immunomagnetic separation (IMS) beads were used to assist the accumulation of bacteria into the path of the beam of the excitation laser. With this combination we have detected Listeria with gold enhanced SERS in a label free manner from such low sample concentrations as 104 CFU ml-1.
Original languageEnglish
Pages (from-to)62981-62989
JournalRSC Advances
Volume6
Issue number67
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

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Listeria
Gold
Laser excitation
Nanoparticles
Bacteria
Substrates
Wavelength
Polymerase chain reaction
Pathogens
Silicon
Polydimethylsiloxane
Labels
Polymers
Metals
Personnel
Infrared radiation
Fabrication
Glass
Costs

Cite this

Uusitalo, Sanna ; Kögler, Martin ; Välimaa, Anna Liisa ; Popov, Alexey P. ; Ryabchikov, Yu V. ; Kontturi, Ville ; Siitonen, Samuli ; Petäjä, Jarno ; Virtanen, T. ; Laitinen, R. ; Kinnunen, Matti ; Meglinski, I. V. ; Kabashin, Andrei V. ; Bunker, Alex ; Viitala, Tapani J. S. ; Hiltunen, Jussi A. / Detection of: Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles. In: RSC Advances. 2016 ; Vol. 6, No. 67. pp. 62981-62989.
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abstract = "The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been studied for the detection of foodborne microbes. The majority of the approaches are either based on costly patterning techniques on silicon or glass wafers or on methods which have not been tested in large scale fabrication. We demonstrate the feasibility of analyte specific sensing using mass-produced, polymer-based low-cost SERS substrate in analysing the chosen model microbe with biological recognition. The use of this novel roll-to-roll fabricated SERS substrate was combined with optimised gold nanoparticles to increase the detection sensitivity. Distinctive SERS spectral bands were recorded for Listeria innocua ATCC 33090 using an in-house build (785 nm) near infra red (NIR) Raman system. Results were compared to both those found in the literature and the results obtained from a commercial time-gated Raman system with a 532 nm wavelength laser excitation. The effect of the SERS enhancer metal and the excitation wavelength on the detected spectra was found to be negligible. The hypothesis that disagreements within the literature regarding bacterial spectra results from conditions present during the detection process has not been supported. The sensitivity of our SERS detection was improved through optimization of the concentration of the sample inside the hydrophobic polydimethylsiloxane (PDMS) wells. Immunomagnetic separation (IMS) beads were used to assist the accumulation of bacteria into the path of the beam of the excitation laser. With this combination we have detected Listeria with gold enhanced SERS in a label free manner from such low sample concentrations as 104 CFU ml-1.",
author = "Sanna Uusitalo and Martin K{\"o}gler and V{\"a}limaa, {Anna Liisa} and Popov, {Alexey P.} and Ryabchikov, {Yu V.} and Ville Kontturi and Samuli Siitonen and Jarno Pet{\"a}j{\"a} and T. Virtanen and R. Laitinen and Matti Kinnunen and Meglinski, {I. V.} and Kabashin, {Andrei V.} and Alex Bunker and Viitala, {Tapani J. S.} and Hiltunen, {Jussi A.}",
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Uusitalo, S, Kögler, M, Välimaa, AL, Popov, AP, Ryabchikov, YV, Kontturi, V, Siitonen, S, Petäjä, J, Virtanen, T, Laitinen, R, Kinnunen, M, Meglinski, IV, Kabashin, AV, Bunker, A, Viitala, TJS & Hiltunen, JA 2016, 'Detection of: Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles', RSC Advances, vol. 6, no. 67, pp. 62981-62989. https://doi.org/10.1039/c6ra08313g

Detection of: Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles. / Uusitalo, Sanna; Kögler, Martin; Välimaa, Anna Liisa; Popov, Alexey P.; Ryabchikov, Yu V.; Kontturi, Ville; Siitonen, Samuli; Petäjä, Jarno; Virtanen, T.; Laitinen, R.; Kinnunen, Matti; Meglinski, I. V.; Kabashin, Andrei V.; Bunker, Alex; Viitala, Tapani J. S.; Hiltunen, Jussi A. (Corresponding Author).

In: RSC Advances, Vol. 6, No. 67, 2016, p. 62981-62989.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Detection of: Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

AU - Uusitalo, Sanna

AU - Kögler, Martin

AU - Välimaa, Anna Liisa

AU - Popov, Alexey P.

AU - Ryabchikov, Yu V.

AU - Kontturi, Ville

AU - Siitonen, Samuli

AU - Petäjä, Jarno

AU - Virtanen, T.

AU - Laitinen, R.

AU - Kinnunen, Matti

AU - Meglinski, I. V.

AU - Kabashin, Andrei V.

AU - Bunker, Alex

AU - Viitala, Tapani J. S.

AU - Hiltunen, Jussi A.

PY - 2016

Y1 - 2016

N2 - The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been studied for the detection of foodborne microbes. The majority of the approaches are either based on costly patterning techniques on silicon or glass wafers or on methods which have not been tested in large scale fabrication. We demonstrate the feasibility of analyte specific sensing using mass-produced, polymer-based low-cost SERS substrate in analysing the chosen model microbe with biological recognition. The use of this novel roll-to-roll fabricated SERS substrate was combined with optimised gold nanoparticles to increase the detection sensitivity. Distinctive SERS spectral bands were recorded for Listeria innocua ATCC 33090 using an in-house build (785 nm) near infra red (NIR) Raman system. Results were compared to both those found in the literature and the results obtained from a commercial time-gated Raman system with a 532 nm wavelength laser excitation. The effect of the SERS enhancer metal and the excitation wavelength on the detected spectra was found to be negligible. The hypothesis that disagreements within the literature regarding bacterial spectra results from conditions present during the detection process has not been supported. The sensitivity of our SERS detection was improved through optimization of the concentration of the sample inside the hydrophobic polydimethylsiloxane (PDMS) wells. Immunomagnetic separation (IMS) beads were used to assist the accumulation of bacteria into the path of the beam of the excitation laser. With this combination we have detected Listeria with gold enhanced SERS in a label free manner from such low sample concentrations as 104 CFU ml-1.

AB - The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been studied for the detection of foodborne microbes. The majority of the approaches are either based on costly patterning techniques on silicon or glass wafers or on methods which have not been tested in large scale fabrication. We demonstrate the feasibility of analyte specific sensing using mass-produced, polymer-based low-cost SERS substrate in analysing the chosen model microbe with biological recognition. The use of this novel roll-to-roll fabricated SERS substrate was combined with optimised gold nanoparticles to increase the detection sensitivity. Distinctive SERS spectral bands were recorded for Listeria innocua ATCC 33090 using an in-house build (785 nm) near infra red (NIR) Raman system. Results were compared to both those found in the literature and the results obtained from a commercial time-gated Raman system with a 532 nm wavelength laser excitation. The effect of the SERS enhancer metal and the excitation wavelength on the detected spectra was found to be negligible. The hypothesis that disagreements within the literature regarding bacterial spectra results from conditions present during the detection process has not been supported. The sensitivity of our SERS detection was improved through optimization of the concentration of the sample inside the hydrophobic polydimethylsiloxane (PDMS) wells. Immunomagnetic separation (IMS) beads were used to assist the accumulation of bacteria into the path of the beam of the excitation laser. With this combination we have detected Listeria with gold enhanced SERS in a label free manner from such low sample concentrations as 104 CFU ml-1.

U2 - 10.1039/c6ra08313g

DO - 10.1039/c6ra08313g

M3 - Article

VL - 6

SP - 62981

EP - 62989

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 67

ER -