Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein

Michel Schmidt, Christer Lindqvist, Aimo Salmi, Christian Oker-Blom (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.
Original languageEnglish
Pages (from-to)216-218
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume3
Issue number2
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Rubella virus
Baculoviridae
Viruses
Immunoglobulin M
Antibodies
Proteins
Fluoroimmunoassay
Chromatography
Insects
Glycoproteins
Antigens

Cite this

Schmidt, Michel ; Lindqvist, Christer ; Salmi, Aimo ; Oker-Blom, Christian. / Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein. In: Clinical and Diagnostic Laboratory Immunology. 1996 ; Vol. 3, No. 2. pp. 216-218.
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Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein. / Schmidt, Michel; Lindqvist, Christer; Salmi, Aimo; Oker-Blom, Christian (Corresponding Author).

In: Clinical and Diagnostic Laboratory Immunology, Vol. 3, No. 2, 1996, p. 216-218.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein

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AU - Salmi, Aimo

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N1 - Project code: B5SU00171

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AB - The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.

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