Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein

Michel Schmidt; Christer Lindqvist; Aimo Salmi; Christian Oker-Blom

doi:10.1128/cdli.3.2.216-218.1996

Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein

Michel Schmidt
, Christer Lindqvist
, Aimo Salmi
, Christian Oker-Blom^*

^*Corresponding author for this work

VTT Technical Research Centre of Finland

University of Turku
Åbo Akademi University
VTT (former employee or external)

Research output: Contribution to journal › Article › Scientific › peer-review

8 Citations (Scopus)

Abstract

The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.

Original language	English
Pages (from-to)	216-218
Journal	Clinical and Diagnostic Laboratory Immunology
Volume	3
Issue number	2
DOIs	https://doi.org/10.1128/cdli.3.2.216-218.1996
Publication status	Published - 1996
MoE publication type	A1 Journal article-refereed

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10.1128/cdli.3.2.216-218.1996

https://europepmc.org/article/MED/8991639

Cite this

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title = "Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein",

abstract = "The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.",

author = "Michel Schmidt and Christer Lindqvist and Aimo Salmi and Christian Oker-Blom",

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AU - Lindqvist, Christer

AU - Salmi, Aimo

AU - Oker-Blom, Christian

N1 - Project code: B5SU00171

PY - 1996

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N2 - The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.

AB - The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.

U2 - 10.1128/cdli.3.2.216-218.1996

DO - 10.1128/cdli.3.2.216-218.1996

M3 - Article

SN - 1071-412X

VL - 3

SP - 216

EP - 218

JO - Clinical and Diagnostic Laboratory Immunology

JF - Clinical and Diagnostic Laboratory Immunology

IS - 2

ER -

Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein

Abstract

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