Detection of siderophores in growing cultures of Pseudomonas spp.

Laura Raaska, Liisa Viikari, Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.

Original languageEnglish
Pages (from-to)181 - 186
Number of pages6
JournalJournal of Industrial Microbiology
Volume11
Issue number3
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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Siderophores
Pseudomonas
Assays
Iron
Detergents
Toxicity
Pseudomonas fluorescens
Wavelength

Cite this

Raaska, Laura ; Viikari, Liisa ; Mattila-Sandholm, Tiina. / Detection of siderophores in growing cultures of Pseudomonas spp. In: Journal of Industrial Microbiology. 1993 ; Vol. 11, No. 3. pp. 181 - 186.
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abstract = "The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.",
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Detection of siderophores in growing cultures of Pseudomonas spp. / Raaska, Laura; Viikari, Liisa; Mattila-Sandholm, Tiina.

In: Journal of Industrial Microbiology, Vol. 11, No. 3, 1993, p. 181 - 186.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Detection of siderophores in growing cultures of Pseudomonas spp.

AU - Raaska, Laura

AU - Viikari, Liisa

AU - Mattila-Sandholm, Tiina

N1 - Project code: ELI28191

PY - 1993

Y1 - 1993

N2 - The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.

AB - The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.

U2 - 10.1007/BF01583720

DO - 10.1007/BF01583720

M3 - Article

VL - 11

SP - 181

EP - 186

JO - Journal of industrial microbiology and biotechnology

JF - Journal of industrial microbiology and biotechnology

SN - 1367-5435

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