Detection of siderophores in growing cultures of Pseudomonas spp.

Laura Raaska; Liisa Viikari; Tiina Mattila-Sandholm

doi:10.1007/BF01583720

Detection of siderophores in growing cultures of Pseudomonas spp.

Laura Raaska, Liisa Viikari, Tiina Mattila-Sandholm

VTT Technical Research Centre of Finland

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27 Citations (Scopus)

Abstract

The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.

Original language	English
Pages (from-to)	181-186
Journal	Journal of Industrial Microbiology
Volume	11
Issue number	3
DOIs	https://doi.org/10.1007/BF01583720
Publication status	Published - 1993
MoE publication type	A1 Journal article-refereed

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10.1007/BF01583720

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title = "Detection of siderophores in growing cultures of Pseudomonas spp.",

abstract = "The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.",

author = "Laura Raaska and Liisa Viikari and Tiina Mattila-Sandholm",

note = "Project code: ELI28191",

year = "1993",

doi = "10.1007/BF01583720",

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journal = "Journal of industrial microbiology and biotechnology",

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T1 - Detection of siderophores in growing cultures of Pseudomonas spp.

AU - Raaska, Laura

AU - Viikari, Liisa

AU - Mattila-Sandholm, Tiina

N1 - Project code: ELI28191

PY - 1993

Y1 - 1993

N2 - The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.

AB - The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.

U2 - 10.1007/BF01583720

DO - 10.1007/BF01583720

M3 - Article

SN - 1367-5435

VL - 11

SP - 181

EP - 186

JO - Journal of industrial microbiology and biotechnology

JF - Journal of industrial microbiology and biotechnology

IS - 3

ER -

Detection of siderophores in growing cultures of Pseudomonas spp.

Abstract

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