Detection of siderophores in growing cultures of Pseudomonas spp.

Laura Raaska, Liisa Viikari, Tiina Mattila-Sandholm

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24 Citations (Scopus)

Abstract

The siderophores produced by Pseudomonas fluorescens and P. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detect Pseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25 μM. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production.

Original languageEnglish
Pages (from-to)181 - 186
Number of pages6
JournalJournal of Industrial Microbiology
Volume11
Issue number3
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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